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Mouse Models to Study GCAP Functions In Intact Photoreceptors

  • Chapter
Photoreceptors and Calcium

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 514))

Abstract

In photoreceptor cells cGMP is the second messenger that transduces light into an electrical response. Regulation of cGMP synthesis by Ca2+is one of the key mechanisms by which Caz+exerts negative feedback to the phototransduction cascade in the process of light adaptation.This Ca2+feedback to retinal guanylyl cyclases (Ret-GCs) is conferred by the guanylate cyclase-activating proteins (GCAPs). Mutations in GCAP1 that disrupt the Ca2+regulation of Ret-GCs in vitro have been associated with severe human vision disorders. This chapter focuses on recent data obtained from biochemical and electrophysiological studies of GCAP I /GCAP2 knockout mice and other GCAP transgenic mice, addressing:

1. the quantitative aspects of the Ca2+-feedback to Ret-GCs in regulating the light sensitivity and adaptation in intact rods;

2. functional differences between GCAPI and GCAP2 in intact rod photore-ceptors; and

3. whether GCAP mutants with impaired Ca2+ binding lead to retinal disease in vivo by constitutive activation ofRet-GCs and elevation of intracellular cOMP, as predicted from in vitro studies.

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Authors
  1. Ana Mendez
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  2. Jeannie Chen
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Editors and Affiliations

  1. Moran Eye Center/EIHG, University of Utah Health Science Center, 15 North/ 2030 East, Salt Lake City, UT, 84112-5330, USA

    Wolfgang Baehr Ph.D.

  2. Department of Ophthalmology, University of Washington, 1959 NE Pacific St., Seattle, WA, 98195, USA

    Krzysztof Palczewski Ph.D.

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Mendez, A., Chen, J. (2002). Mouse Models to Study GCAP Functions In Intact Photoreceptors. In: Baehr, W., Palczewski, K. (eds) Photoreceptors and Calcium. Advances in Experimental Medicine and Biology, vol 514. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0121-3_22

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  • DOI: https://doi.org/10.1007/978-1-4615-0121-3_22

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4933-4

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