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Structure and Membrane-Targeting Mechanism of Retinal Ca2+-Binding Proteins, Recoverin and GCAP-2

  • B. Ames
  • Mitsuhiko Ikura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 514)

Abstract

Recoverin and the guanylate cyclase activating proteins (GCAPs) are calcium-sensing proteins in retinal rod and cone cells that belong to the EF-hand superfamily and serve as important calcium sensors in vision. Recoverin and the GCAP proteins are myristoylated at their amino-terminus and are targeted to retinal disc membranes by a myristoyl switch. Here, we present the three-dimensional, atomic-resolution structures of recombinant myristoylated recoverin containing 0, 1 and 2 calcium ions (Ca2+) bound and unmyristoylated GCAP-2 with 3 Ca2+bound as determined by nuclear magnetic resonance. The Ca2+-induced structural changes in these proteins are important for elucidating their membrane-targeting mechanisms and for understanding the molecular mechanism of Ca2+-sensitive regulation of phototransduction.

Keywords

Root Mean Square Deviation Guanylate Cyclase Guanylyl Cyclase Domain Interface Main Chain Atom 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • B. Ames
    • 1
  • Mitsuhiko Ikura
    • 2
  1. 1.Center for Advanced Research in BiotechnologyUniversity of Maryland Biotechnology InstituteRockville
  2. 2.Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical BiophysicsUniversiy of Toronto, 610 University AvenueTorontoCanada

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