Structure and Membrane-Targeting Mechanism of Retinal Ca2+-Binding Proteins, Recoverin and GCAP-2

  • B. Ames
  • Mitsuhiko Ikura
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 514)


Recoverin and the guanylate cyclase activating proteins (GCAPs) are calcium-sensing proteins in retinal rod and cone cells that belong to the EF-hand superfamily and serve as important calcium sensors in vision. Recoverin and the GCAP proteins are myristoylated at their amino-terminus and are targeted to retinal disc membranes by a myristoyl switch. Here, we present the three-dimensional, atomic-resolution structures of recombinant myristoylated recoverin containing 0, 1 and 2 calcium ions (Ca2+) bound and unmyristoylated GCAP-2 with 3 Ca2+bound as determined by nuclear magnetic resonance. The Ca2+-induced structural changes in these proteins are important for elucidating their membrane-targeting mechanisms and for understanding the molecular mechanism of Ca2+-sensitive regulation of phototransduction.


Root Mean Square Deviation Guanylate Cyclase Guanylyl Cyclase Domain Interface Main Chain Atom 
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Copyright information

© Springer Science+Business Media New York 2002

Authors and Affiliations

  • B. Ames
    • 1
  • Mitsuhiko Ikura
    • 2
  1. 1.Center for Advanced Research in BiotechnologyUniversity of Maryland Biotechnology InstituteRockville
  2. 2.Division of Molecular and Structural Biology, Ontario Cancer Institute and Department of Medical BiophysicsUniversiy of Toronto, 610 University AvenueTorontoCanada

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