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New Trends in Cancer for the 21st Century pp 59–68Cite as

Proteomic Approaches to the Diagnosis, Treatment, and Monitoring of Cancer

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Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 532))

Abstract

The field of proteomics holds promise for the discovery of new biomarkers for the early detection and diagnosis of disease, molecular targets for therapy and markers for therapeutic efficacy and toxicity. A variety of proteomics approaches may be used to address these goals. Two-dimensional gel electrophoresis (2D-PAGE) is the cornerstone of many discovery-based proteomics studies. Technologies such as laser capture microdissection (LCM) and highly sensitive MS methods are currently being used together to identify greater numbers of lower abundance proteins that are differentially expressed between defined cell populations. Newer technologies such as reverse phase protein arrays will enable the identification and profiling of target pathways in small biopsy specimens. Surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) analysis enables the high throughput characterization of lysates from very few tumor cells or body fluids and may be best suited for diagnosis and monitoring of disease. Such technologies are expected to supplement our arsenal of mRNA-based assays, and we believe that in the future, entire cellular networks and not just a single deregulated protein will be the target of therapeutics and that we will soon be able to monitor the status of these pathways in diseased cells before, during and after therapy.

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Author information

Authors and Affiliations

  1. FDA/NCI Clinical Proteomics Program, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, 20892, USA

    Julia D. Wulfkuhle Ph.D., Cloud P. Paweletz & Lance Liotta

  2. Women’s Cancers Section, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, Bethesda, MD, 20892, USA

    Patricia S. Steeg

  3. FDA/NCI Clinical Proteomics Program, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, MD, 20892, USA

    Emanuel F. Petricoin III

  4. Department of Anatomy, Physiology, and Genetics, Institute for Molecular Medicine, Uniformed Services School of Medicine, Bethesda, MD, 20814, USA

    Cloud P. Paweletz

Authors
  1. Julia D. Wulfkuhle Ph.D.
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  2. Cloud P. Paweletz
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  3. Patricia S. Steeg
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  4. Emanuel F. Petricoin III
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  5. Lance Liotta
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Corresponding author

Correspondence to Julia D. Wulfkuhle Ph.D. .

Editor information

Editors and Affiliations

  1. University of Valencia, Valencia, Spain

    Antonio Llombart-Bosch

  2. Valencian Foundation for Biomedical Research, Valencia, Spain

    Vicente Felipo

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© 2003 Springer Science+Business Media New York

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Wulfkuhle, J.D., Paweletz, C.P., Steeg, P.S., Petricoin, E.F., Liotta, L. (2003). Proteomic Approaches to the Diagnosis, Treatment, and Monitoring of Cancer. In: Llombart-Bosch, A., Felipo, V. (eds) New Trends in Cancer for the 21st Century. Advances in Experimental Medicine and Biology, vol 532. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0081-0_7

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  • DOI: https://doi.org/10.1007/978-1-4615-0081-0_7

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4914-3

  • Online ISBN: 978-1-4615-0081-0

  • eBook Packages: Springer Book Archive

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