Taurine 5 pp 25-31 | Cite as

Cellular Characterization of Taurine Transporter in Cultured Cardiac Myocytes and Nonmyocytes

  • Tomoka Takatani
  • Kyoko Takahashi
  • Takashi Itoh
  • Koichi Takahashi
  • Mayo Hirata
  • Yasuhiro Yamamoto
  • Masanori Ohmoto
  • Stephen W. Schaffer
  • Junichi Azuma
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 526)

Abstract

Taurine is by far the most abundant free amino acid in the mammalian heart, comprising in excess of 50% of the total free amino acid pool1,2. Although its physiological function remains undefined, taurine exhibits an extensive cardiovascular pharmacology. Carnivores depend to a large extent on taurine obtained through the diet, which must be transported across cell membranes to accumulate in the heart. The taurine transporter, which belongs to a gene family that encodes Na+-and Cl--coupled transporters2,3has been cloned from mammalian tissues and cells3–10. Although numerous cell types are present in the heart, cardiomyocytes and cardiac fibroblasts are the predominant cell type in the neonatal rat heart. There are no known studies showing the detailed characterization of the taurine transporter in cardiac cells. The present study was undertaken to clarify the physiological characteristics of the cardiac taurine transporter using cultured myocytes and nonmyocytes prepared from neonatal rats.

Keywords

Polypeptide Angiotensin Alanine Taurine Century Edit 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Pisarenko OI, 1996, Mechanisms of myocrdial protection by amino acids: Facts and hypothesis.Clin. Exp. Pharmacol. Physiol.23: 627–633.CrossRefPubMedGoogle Scholar
  2. 2.
    Huxtable R J, Sebring L, 1982, Cardiovascular actions of taurine.Amino Acids5:29–51.Google Scholar
  3. 3.
    Uchida S, Kwon HM, Yamauchi A, Preson AS, Marumo F, Handler JS, 1992, Molecular cloning of the cDNA for an MDCK cell Na+-and Cf.-dependent taurine transporter that is regulated by hypertonicity.Proc. Natl. Acad. Sci.89: 8230–8234.CrossRefPubMedGoogle Scholar
  4. 4.
    Smith ICE, Borden LA, Wang CD, Hartig PR, Branchek TA, Weinshank RL, 1992, Cloning and expression of a high affinity taurine transporter from rat brain.Mol. Pharmacol.42: 563–569.PubMedGoogle Scholar
  5. 5.
    Liu 0, L-Corcuera B, Nelson H, Mandiyan S, Nelson N, 1992, Cloning and expression of a cDNA encoding the transporter of taurine and 0-alanine in mouse brain.Proc. Natl. Acad. Sci.89: 12145–12149.CrossRefGoogle Scholar
  6. 6.
    Jhiang SM, Fithian L, Smanik P, McGill J, Tong Q, Mazzaferri EL, 1993, Cloning of the human taurine transporter and characterization of taurine uptake in thyroid cells.FEBS Lett.318: 139–144.CrossRefPubMedGoogle Scholar
  7. 7.
    Ramamoorthy S, Leibach FH, Mahesh VB, Han H, Yang-Feng T, Blakely RD, Ganapathy V, 1994, Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta.Biochem. J.300: 893–900.PubMedGoogle Scholar
  8. 8.
    Jayanthi LD, Ramamoorthy S, Mahesh VB, Leibach FH, Ganapathy V, 1995, Substrate-specific regulation of the taurine transporter in human placental choriocarcinoma cells (JAR).Biochim. Biophys. Acta.1235: 351–360.CrossRefPubMedGoogle Scholar
  9. 9.
    Miyamoto Y, Liou GI, Sprinkle TJ, 1996, Isolation of a cDNA encoding a taurine transporter in the human retinal pigment epithelium.Curr. Eye Res.15: 345–349.CrossRefPubMedGoogle Scholar
  10. 10.
    Leibach JW, Cool DR, Monte MAD, Ganapathy V, Leibach FH, Miyamoto Y, 1993, Properties of taurine transport in a human retinal pigment epithelial cell line.Curr. Eye Res.12: 29–36.CrossRefPubMedGoogle Scholar
  11. 11.
    Sadoshima J, Lzumo S, 1993, Molecular characterization of angiotensin II-induced hypertrophy of cardiac myocytes and hyperplasia of cardiac fibroblasts.Circ. Res.73: 416–423.Google Scholar
  12. 12.
    Jones BN, Gilligan JP, 1983, O-phthaladehyde precolumn derivatization and reversed-phase high-performance liquid chromatography of polypeptide hydrolysates and physiological fluids.J. Chromatogr.266: 471–482.CrossRefPubMedGoogle Scholar
  13. 13.
    Chomczynski P, Sacchi N, 1987, Single-step method of RNA isolation by acid guanidium thiocyanate-phenol-chloroform extraction.Anal. Biochem.162: 156–159.CrossRefPubMedGoogle Scholar
  14. 14.
    Kim S, Ohta k, Hamaguchi A, Yukimura T, Miura K, Iwao H, 1995, Angiotensin II induces cardiac phenotypic modulation and remodeling in vivo in rats.Hypertension25: 1252–1259.CrossRefPubMedGoogle Scholar
  15. 15.
    Mollerup J, Lambert IH, 1998, Calyculin A modulates the kinetic constants for Na+-coupled taurine transport in Ehrlich ascites tumour cells.Biochim. Biophys. Acta. 1371: 335–344.CrossRefPubMedGoogle Scholar
  16. 16.
    Takahashi K, Azuma M, Taira K, Baba A, Yamamoto Y, Schaffer SW, Azuma J, 1997, Effect of taurine on anigotensin II-induced hypertrophy of neonatal rat cardiac cells.J. Cardiovasc. Pharmacol.30: 725–730.CrossRefPubMedGoogle Scholar
  17. 17.
    Takahashi K, Schaffer SW, Azuma J, 1997, Taurine prevents intracellular calcium overload during calcium paradox of cultured cardiomyocytes.Amino Acids13: 1–11.CrossRefGoogle Scholar
  18. 18.
    Schaffer SW, Azuma J, Madura JD, 1995, Mechanisms underlying taurine-mediated alterations in membrane function.Amino Acids8: 231–46.CrossRefGoogle Scholar
  19. 19.
    Kundaiker S, Hussain AA, Marshall J, 1996, Component characteristics of the vectorial transport system for taurine in isolated bovine retinal pigment epithelium.J. Physiol.492: 505–516.PubMedGoogle Scholar
  20. 20.
    Ganapathy V, Leibach FH, 1994, Expression and regulation of the taurine transporter in cultured cell lines of human origin. In: Taurine in Health and Disease, (Eds. R. Huxtable and DV Michalk), pp 51–57. Plenum Press, New York.Google Scholar

Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Tomoka Takatani
    • 1
  • Kyoko Takahashi
    • 1
  • Takashi Itoh
    • 1
  • Koichi Takahashi
    • 2
  • Mayo Hirata
    • 1
  • Yasuhiro Yamamoto
    • 1
  • Masanori Ohmoto
    • 1
  • Stephen W. Schaffer
    • 3
  • Junichi Azuma
    • 1
  1. 1.Clinical Evaluation of Medicines and Therapeutics, Graduate School of Pharmaceutical SciencesOsaka UniversityOsakaJapan
  2. 2.Department of Pharmaceutics, School of Pharmaceutical SciencesMukogawa Women’s UniversityHyogoJapan
  3. 3.Department of PharmacologyUniversity of South Alabama, School of MedicineUSA

Personalised recommendations