Skip to main content

Cloning of Human Intestinal Taurine Transporter and Production of Polyclonal Antibody

  • Chapter
Taurine 5

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 526))

Abstract

Taurine (2-ethaneaminosulfonic acid, +NH3CH2CH2SO3) is an endogenous amino acid which functions as a modulator of transmembrane calcium transport, an osmoregulator and, putatively a free radical scavenger1. In utero, taurine deficiency causes an abnormal development of brain, retina, kidney, and abnormal function of the myocardium, where taurine plays an important role in intracellular calcium homeostasis in the cardiomyocytes2–4.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 169.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 219.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 219.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Chesney, R.W., and Budreau, A., 1993, Efflux of taurine from renal brush border membrane vesicles: Is it adaptively regulated?. Pediatr. Nephrol. 7: 35–40.

    Article  PubMed  CAS  Google Scholar 

  2. Chesney, R.W., Gusowski, N., and Dabbagh, S., 1985, Renal cortex taurine content regulates renal adaptive response to altered dietary intake of sulfur amino acids. J. Clin. Invest. 76: 2213–2221.

    Article  PubMed  CAS  Google Scholar 

  3. Chesney, R.W., Gusowski, N., and Freidman, A.L., 1983, Renal adaptation to altered amino acid intake occurs at the luminal brush border membrane. Kidney Int. 24: 588–594.

    Article  PubMed  CAS  Google Scholar 

  4. Chesney, R.W., Helms, R.A., Christensen, M., Budreau, A.M., Han, X., and Sturman, J.A., 1998, An updated view of the value of taurine in infant nutrition. In Advances in Pediatrics,edited by Barnes La, pp. 179–193.

    Google Scholar 

  5. Uchida, S., Kwon, H.M., Yamauchi, A., Preston, A.S., Marumo, F., and Handler, J.S., 1992, Molecular cloning of the cDNA for an MDCK cell Nat-and CF-dependent taurine transporter that is regulated by hypertonicity. Proc. Natl. Acad. Sei. USA 89: 8230–8234.

    Article  CAS  Google Scholar 

  6. Smith, K.E., Borden, L.A., Wang, C.D., Hartig, P.R., Branchek, T.A., and Weinshank, R.L., 1992, Cloning and expression of a high affinity taurine transporter from rat brain. Mol. Pharmacol. 42: 563–569.

    PubMed  CAS  Google Scholar 

  7. Liu, Q.R., Lopez-Corcuera, B., Nelson, H., Mandiyan, S., and Nelson, N., 1992, Cloning and expression of a cDNA encoding the transporter of taurine and 13-alanine in mouse brain. Proc. Natl. Acad. Sci. USA 89: 12145–12149.

    Article  PubMed  CAS  Google Scholar 

  8. Vinnakota, S., Qian, X., Egal, H., Sarthy, V., and Sarkar, H.K., 1997, Molecular characterization and in situ localization of a mouse retinal taurine transporter. J. Neurochem. 69: 2238–2250.

    Article  PubMed  CAS  Google Scholar 

  9. Han, W., Budreau, A.M., and Chesney, R.W., 1998, Molecular cloning and functional expression of an LLC-PK1 cell taurine transporter that is adaptively regulated by taurine. Adv. Exp. Med. Biol. 442: 261–268.

    PubMed  CAS  Google Scholar 

  10. Jhiang, S.M., Fithian, L., Smanik, P., McGill, J., Tong, Q., and Mazzaferri, E.L., 1993, Cloning of the human taurine transporter and characterization of taurine uptake in thyroid cells. FEBS Lett. 318: 139–144.

    Article  PubMed  CAS  Google Scholar 

  11. Ramamoorthy, S., Leibach, F.H., Mahesh, V.B., Han, H., Yang-Feng, T., Blakely, R.D., and Ganapathy, V., 1994, Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta. Biochem. J. 300: 893–900.

    PubMed  CAS  Google Scholar 

  12. Miyamoto, Y., Lion, G.I., and Sprinkle, T.J., 1996, Isolation of a cDNA encoding a taurine transporter in the human retinal pigment epithelium. Curr. Eye Res. 15: 345–349.

    Article  PubMed  CAS  Google Scholar 

  13. Takeuchi, K., Toyohara, H., and Sakaguchi, M., 2000, A hyperosmotic stress-induced mRNA of carp cell encodes Na+- and Cl-dependent high affinity taurine transporter. Biochim. Biophys. Acta. 1464: 219–230.

    Article  PubMed  CAS  Google Scholar 

  14. Han, X., Budreau, A.M., and Chesney, R.W., 1999, Ser-322 is a critical site for PKC regulation of the MDCK cell taurine transporter (pNCT). J. Am. Soc. Nephrol. 10: 1874–1879.

    PubMed  CAS  Google Scholar 

  15. Tebele, N., Skilton, R.A., Katende, J., Wells, C.W., Nene, V., McElwain, T., Morzaria, S.P., and Musoke, A.J., 2000, Cloning, characterization, and expression of a 200-kilodalton diagnostic antigen of Babesia bigemina. J. Clin. Microbiol. 38(6): 2240–2247.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2003 Springer Science+Business Media New York

About this chapter

Cite this chapter

An, H.S., Han, H.C., Park, T., Park, K.K., Kim, H.W. (2003). Cloning of Human Intestinal Taurine Transporter and Production of Polyclonal Antibody. In: Lombardini, J.B., Schaffer, S.W., Azuma, J. (eds) Taurine 5. Advances in Experimental Medicine and Biology, vol 526. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0077-3_17

Download citation

  • DOI: https://doi.org/10.1007/978-1-4615-0077-3_17

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4913-6

  • Online ISBN: 978-1-4615-0077-3

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics