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The Oxygen Dependency of Cerebral Oxidative Metabolism in the Newborn Piglet Studied with 31P NMRS and NIRS

  • Roger J Springett
  • Marzena Wylezinska
  • Ernest B. Cady
  • Veronica Hollis
  • Mark Cope
  • David T. Delpy
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 530)

Abstract

Mean cerebral saturation and changes in the oxidation state of the CuA centre of cytochrome oxidase were measured by near infra-red spectroscopy simultaneously with phosphorous metabolites and intracellular pH measured using 31P NMR spectroscopy during transient anoxia (inspired oxygen fraction = 0.0 for 105 seconds) in the newborn piglet brain. By collecting high quality 31 P spectra every 10 seconds, it was possible to resolve the delay between the onset of anoxia and the fall in PCr and to show that the CuA centre of cytochrome oxidase reduced simultaneously with the fall in PCr. From these observations it is concluded that, at normoxia, oxygen tension at the mitochondrial level is substantially above a critical value at which oxidative metabolism becomes oxygen dependent.

Key words

NIRS 31P NMRS cytochrome oxidase hypoxia 

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Copyright information

© Springer Science+Business Media New York 2003

Authors and Affiliations

  • Roger J Springett
    • 1
  • Marzena Wylezinska
    • 2
  • Ernest B. Cady
    • 2
  • Veronica Hollis
    • 3
  • Mark Cope
    • 3
  • David T. Delpy
    • 3
  1. 1.Department of RadiologyDartmouth CollegeHanoverUSA
  2. 2.University College Hospitals NHS TrustLondonUK
  3. 3.Department of Medical Physics & BioengineeringUniversity College LondonLondonUK

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