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Investigating the Role of Nitric Oxide in Regulating Blood Flow and Oxygen Delivery from in Vivo Electrochemical Measurements in Eye and Brain

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Oxygen Transport to Tissue XXIV

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 530))

Abstract

We have previously shown from direct, in vivo measurements of NO in cats with recessed electrochemical microsensors that NO mediates increases in ONH blood flow during functional activation of the eye by flickering light. We have also reported that there are low frequency (< 15 cycles/min) spontaneous oscillations in NO that appear to be passively coupled to oscillations in blood flow at similar frequencies in the cat ONH. In this paper, we describe similarities between in vivo measurements of NO in the ONH of the cat eye and in the cortex of the rat brain. These data are consistent with a role for NO in the coupling of blood flow with increases in neuronal activity, autoregulation of blood flow, hyperemia, and vasodilation during hypoxia and hypercapnia.

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Abbreviations

EEG:

electroencephalograph

IOP:

intra-ocular pressure

Km :

Michaelis-Menten constant

LDF:

laser Doppler flowmetry

L-NAME:

Nω-nitro-L-arginine methyl ester

7-NI:

7-nitroindazole

NO:

nitric oxide, nitric monoxide

NOS:

nitric oxide synthase(neuronal Nnos, endothelial Enos, inducible iNOS)

ONH:

optic nerve head

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Authors and Affiliations

  1. Departments of Physiology, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA

    Donald G. Buerk

  2. Departments of Bioengineering, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA

    Donald G. Buerk

  3. Institute for Environmental Medicine, University of Pennsylvania School of Medicine, Philadelphia, PA, 19104, USA

    Donald G. Buerk & Dmitriy N. Atochin

  4. Institut de Recherches en Ophthalmologie, Sion, Switzerland

    Charles E. Riva

Authors
  1. Donald G. Buerk
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  2. Dmitriy N. Atochin
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  3. Charles E. Riva
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Editor information

Editors and Affiliations

  1. Dartmouth Medical School, Hanover, New Hampshire, USA

    Jeffrey F. Dunn  & Harold M. Swartz  & 

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© 2003 Springer Science+Business Media New York

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Buerk, D.G., Atochin, D.N., Riva, C.E. (2003). Investigating the Role of Nitric Oxide in Regulating Blood Flow and Oxygen Delivery from in Vivo Electrochemical Measurements in Eye and Brain. In: Dunn, J.F., Swartz, H.M. (eds) Oxygen Transport to Tissue XXIV. Advances in Experimental Medicine and Biology, vol 530. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0075-9_33

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  • DOI: https://doi.org/10.1007/978-1-4615-0075-9_33

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4912-9

  • Online ISBN: 978-1-4615-0075-9

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