Abstract
We recently described a novel approach to measuring regional tumor oxygen tension. This approach is based on 19F pulse burst saturation recovery NMR echo planar imaging relaxometry of hexafluorobenzene or “FREDOM” (Fluorocarbon Relaxometry using Echo planar imaging for Dynamic Oxygen Mapping). We have now compared oxygen tension measurements using FREDOM with a traditional polarographic method (the Eppendorf Histograph) in a group of size matched Dunning prostate rat tumors R3327-AT1. We also compare MR and electrode approaches to monitoring dynamic changes with respect to interventions and demonstrate extension of the MR technique to rat breast tumors.
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Abbreviations
- ARDVARC:
-
Alternated Relaxation Delays with Variable Acquisitions to Reduce Clearance effects
- EPI:
-
echo planar imaging
- FREDOM:
-
Fluorocarbon Relaxometry using Echo planar imaging for Dynamic Oxygen Mapping
- HFB:
-
hexafluorobenzene
- i.t:
-
intra tumoral
References
Hall EJ. The oxygen effect and reoxygenation. In: Hall EJ, ed. Radiobiology for the Radiologist. 3 ed. Philadelphia: Lippincott, J. B., 1994;133–152.
Chapman JD, Stobbe CC, Arnfield MR, Santus R, Lee J, McPhee MS. Oxygen Dependency of Tumor Cell Killing In Vitro by Light Activated Photofrin II. Radiat Res 1991;126:73–79.
Brown JM, Giaccia AJ.Tumor hypoxia: the picture has changed in the 1990s. Int J Radiat Biol 1994;65:95–102.
Vaupel PW, Höckel M. Oxygenation status of human tumors: a reappraisal using computerized pO2histography. In: Vaupel PW, Kelleher DK, Günderoth M, eds. Tumor Oxygenation. Stuttgart: Gustav Fischer, 1995;219–232. (Thews G, ed. Funktionsanalyse biologischer Systeme; vol 24).
Fyles AW, Milosevic M, Wong R, et al. Oxygenation predicts radiation response and survival in patients with cervix cancer. Radiother Oncol 1998;48:149–156.
Höckel M, Schlenger K, Aral B, Mitze M, Schäffer U, Vaupel P. Association between tumor hypoxia and malignant progression in advanced cancer of the uterine cervix. Cancer Res 1996;56:4509–4515.
Nordsmark M, Overgaard M, Overgaard J. Pretreatment oxygenation predicts radiation response in advanced squamous cell carcinoma of the head and neck. Radiother Oncol 1996;41:31–40.
Stone HB, Brown JM, Phillips T, Sutherland RM. Oxygen in human tumors: correlations between methods of measurement and response to therapy. Radiat Res 1993;136:422434.
Mason RP, Rodbumrung W, Antich PP. Hexafluorobenzene: a sensitive 19F NMR indicator of tumor oxygenation. NMR in Biomed 1996;9:125–134.
Le D, Mason RP, Hunjan S, Constantinescu A, Barker BR, Antich PP. Regional tumor oxygen dynamics: 19F PBSR EPI of hexafluorobenzene. Magn Reson Imaging 1997;15:971–981.
Mason RP, Constantinescu A, Hunjan S, et al. Regional tumor oxygenation and measurement of dynamic changes. Radiat Res 1999;152:239.
Hunjan S, Mason RP, Constantinescu A, Peschke P, Hahn EW, Antich PP. Regional tumor oximetry: 19F NMR spectroscopy of hexafluorobenzene. Int J Radiat Oncol Biol Phys 1998;40:161–171.
Delpuech J-J, Hamza MA, Serratice G, Stébé M-J. Fluorocarbons as oxygen carriers. I. An NMR study of oxygen solutions in hexafluorobenzene. J Chem Phys 1979;13:399.
Mason RP. Non-invasive physiology: 19F NMR of perfluorocarbon. Art. Cells, Blood Sub & Immob Biotech 1994;22:1141–1153.
Hahn EW, Peschke P, Mason RP, Babcock EE, Antich PP. Isolated tumor growth in a surgically formed skin pedicle in the rat: a new tumor model for NMR studies. Magn Reson Imaging 1993;11:1007–1017.
Laurence V, Ward R, Bleehen N. Tumor pO2 distribution in patients treated with the combination of nicotinamide and carbogen breathing. In: P. W. Vaupel, D. K. Kelleher, M. Günderoth, eds. Tumor Oxygenation. Stuttgart: Gustav Fischer, 1995;185–193.
Cater D, Silver I. Quantitative measurements of oxygen tension in normal tissues and in the tumors of patients before and after radiotherapy. Acta Radiol 1960;53:233–256.
Chaplin DJ, Horsman MR, Siemann DW. Further evaluation of nicotinamide and carbogen as a strategy to reoxgenate hypoxic cells in vivo: importance of nicotinamide dose and pre-irradiation breathing time. Br J Cancer 1993;68:269–273.
Dardzinski BJ, Sotak CH. Rapid tissue oxygen tension mapping using 19F Inversion-recovery Echo-planar imaging of Perfluoro-l5-crown-5-ether. Magn Reson Med 1994;32:88–97.
Baldwin NJ, Ng TC. Oxygenation and metabolic status of KHT tumors as measured simultaneously by 19F magnetic resonance imaging and 31P magnetic resonance spectroscopy. Magn Reson Imaging 1996;14:514–551.
Fishman JE, Joseph PM, Carvlin MJ, Saadi-Elmandjra M, Mukherji B, Sloviter HS. In vivo measurements of vascular oxygen tension in tumors using MRI of a fluorinated blood substitute. Invest Radiol 1989;24:65–71.
Mason RP, Antich PP, Babcock EE, Constantinescu A, Peschke P, Hahn EW. Noninvasive determination of tumor oxygen tension and local variation with growth. Int J Radiat Oncol Biol Phys 1994;29: 95–103.
McIntyre DJO, McCoy CL, Griffiths JR. Tumour oxygenation measurements by 19F MRI of perfluorocarbons. Curr Sci 1999;76:753–762.
Brown SL, Ewing JR, Kolozsvary A, Butt S, Cao Y, Kim JH. Magnetic Resonance Imaging of perfusion in rat cerebral 9L tumor after nicotinamide administration. Int J Radiat Oncol Biol Phys 1999;43:627–633.
Mason R, Hunjan S, He S, et al. Tumor trans membrane pH gradient and regional oxygen tension measured by fluorine magnetic resonance. In: Moraes M, Brentani R, Bevilacqua R, eds. 17th International Cancer Congress. Rio de Janeiro: Monduzzi, 1998;1627–1631. vol 2).
Mason RP. Transmembrane pH gradients in vivo: measurements using fluorinated vitamin B6 derivatives. Curr Med Chem 1999;6:533–551.
Lancaster. Material Safety Data Sheet. In: Lancaster Synthesis Inc., 1998.
Hall LW, Jackson SRK, Massey GM. Hexafluorobenzene in veterinary anaesthesia. In: Arias A, Llaurado R, Nalda MA, Lunn JN, eds. Recent Progress in Anaesthesiology and Resuscitation. Oxford: Excerpta Medica, 1975;201–204.
Rietjens IMCM, Steensma A, den Besten C, et al. Comparative biotransformation of hexachlorobenzene and hexafluorobenzene in relation to the induction of porphyria. Eur J Pharmacol 1995;293:292–299.
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Mason, R.P. et al. (2003). Tumor Oximetry: Comparison of 19F MR EPI and Electrodes. In: Dunn, J.F., Swartz, H.M. (eds) Oxygen Transport to Tissue XXIV. Advances in Experimental Medicine and Biology, vol 530. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0075-9_3
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DOI: https://doi.org/10.1007/978-1-4615-0075-9_3
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