Abstract
Biodegradable materials based on polymers of hydroxy acids are studied for application in artificial vascular substitutes. Polymers with functional surfaces are being developed, carrying specific recognition structures to affect selectively the adhesion and proliferation of endothelial cells (EC) and vascular smooth muscle cells (VSMC). This preliminary study focuses on evaluation of adhesion and growth of VSMC on surfaces of polylactide polymers and those modified by amphiphilic polylactide/poly(ethylene oxide) copolymers. Poly(L-lactic acid), PLLA, and poly(DL-lactic acid), PDLLA, and a block copolymer of lactide with a carboxylated poly(ethylene oxide) segment, PLLA-b-PEO-COOH, were synthesized by controlled polymerization of L and D,L-lactide, respectively, and using 0-hydroxy-Zcarboxymethyl-PEO as a macroinitiator for the copolymer. Films of polymers were deposited on glass coverslips by a spin-coating method. Uncoated glass coverslips and Falcon dishes were used as control substrates. VSMC were obtained from the thoracic aorta of young adult male Wistar rats by explantation method and seeded in Dulbecco-Modified Eagle MEM with 10% foetal bovine serum. The number of adhering cells, their shape, size of cell-material contact area and cell population doubling time were evaluated from day 1 to 7 after seeding. It was found that both PLLA and especially PDLLA relatively well supported adhesion and growth ofVSMC. However, on carboxylated surfaces of the PLLA-b-PEO-COOH copolymer, a lower number of initially adhering cells (by 37% than on Falcon dishes, poO.05), smaller cell spreading area (by 45% and 37% than on glass and Falcon dishes, respectively, pOO.OI) and longer doubling time (by 49% and 31 % than on glass and Falcon dishes, poO.OOI). Thus, surfaces coated by a PLAJPEO-COOH copolymer can be used as minimum background surface to reveal the effect of other more specific adhesion structures.
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References
Greisler, H.P., Tattersall, C.W., Klosak, J.J., Cabusao, E.A., Garfield, J.D., and Kim, D.U., 1991, Partially bioresorbable vascular grafts in dogsSurgery 110645–654
Greisler, H.P., Gosselin, C., Ren, D.W., Kang, S.S., and Kim, D.U., 1996, Biointeractive polymers and tissue engineered blood vessels.Biomaterials 17329.
Bordenave, L., Remy-Zolghadri, M., Fernandez, P., Bareille, R., and Midy, D., 1999, Clinical performance of vascular grafts lined with endothelial cells.Endothelium 6267–275.
Holt, D.B., Eberhart, R.C., and Prager M.D., 1994, Endothelial cell binding to Dacron modified with polyethylene oxide and peptide.ASAIO 40M858–63.
Kim, W.G., Park, J.K., Park, Y.N., Hwang, C.M., Jo, Y.H., Min, B.G., Yoon, C.J., Lee, T.Y., 2000, Tissue-engineered heart valve leaflets: an effective method for seeding autologous cells on scaffolds.Int. J. Artif. Organs 23624–628.
Bačáková, L., Švorčík, V., Rybka, V., Micek, I., Hnatowicz, V., Lisa, V., and Kocourek, F., 1996, Adhesion and proliferation of cultured human aortic smooth muscle cells on polystyrene implanted with N+, F+ and Ar+ ions: correlation with polymer surface polarity and carbonization.Biomaterials 171121–1126.
Hsu, S.H., Tseng, H.J., and Fang, Z.K., 1999, Polyurethane blended with polylactides for improved cell adhesion and reduced platelet activation.Artif. Organs 23958–961.
Noth, U., Tuli, R., Osyczka, A.M., Danielson, K.G., and Tuan, R.S., 2002In vitroengineered cartilage constructs produced by press-coating biodegradable polymer with human mesenchymal stem cells.Tissue Eng. 8131–144.
Han, D.K., Lee, K.B., Park, K.D., Kim, C.S., Jeong, S.Y., Kim, Y.H., Kim, H.M., and Min B.G., 1993In vivocanine studies of a Sinkhole valve and vascular graft coated with biocompatible PU-PEO-SO3.ASAIO J 39M537–M541.
Liu, V.A., Jastromb, W.E., and Bhatia, S.N., 2002, Engineering protein and cell adhesivity using PEO-terminated triblock polymers.J. Biomed. Mater. Res. 60126–134.
Mann, B.K., and West, J.L. (2002). Cell adhesion peptides alter smooth muscle cell adhesion, proliferation, migration, and matrix protein synthesis on modified surfaces and in polymer scaffolds.J. Biomed. Mater. Res. 6086–93.
Kubies, D., Rypáček, F., Kovářová, J., and Lednický, F., 2000, Microdomain structure in polylactide-block-poly(ethylene oxide) copolymer films.Biomaterials 21529–536.
Rypkek, F., Machová, L., Kotva, R., and Škarda, V., 2001, Polyesters with functional-peptide blocks: synthesis and application to biomaterials.Polym. Mater. Sci. Eng. 84817–818.
Van Dijk, J.A.P.P., and Smit J.A.M., 1983, Characterization of Poly(D,L-Lactic Acid) by Gel Permeation Chromatography.J.Polym.Sci. Part A: Polym.Chem. 21197–208.
Bačáková, L., Mareš, V., and Lisá, V., 1999, Gender-related differences in adhesion, growth and differentiation of vascular smooth muscle cells are enhanced in serum-deprived cultures.Cell. Biol. Int. 23643–648.
Bačáková, L., and Kuneš, J., 2000, Gender differences in growth of vascular smooth muscle cells isolated from hypertensive and normotensive rats.Clin. Exp. Hypertens. 2233–44.
Kubies, D., Machová, L., Brynda, E., and Rypáček, F., 2002, Functionalised surfaces of polylactide modified by Langmuir-Blodgett films of amphiphilic block copolymers.J. Mater. Sci Mater. Med.(in press).
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Bačáková, L., Lapčíková, M., Kubies, D., Rypáček, F. (2003). Adhesion and Growth of Rat Aortic Smooth Muscle Cells on Lactide-Based Polymers. In: Elçin, Y.M. (eds) Tissue Engineering, Stem Cells, and Gene Therapies. Advances in Experimental Medicine and Biology, vol 534. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0063-6_14
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DOI: https://doi.org/10.1007/978-1-4615-0063-6_14
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