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In Vivo and in Vitro Detection of NO by EPR

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In Vivo EPR (ESR)

Part of the book series: Biological Magnetic Resonance ((BIMR,volume 18))

Abstract

The aim of this chapter is to review the various methods used to detect NO and an in-depth overview of in vivo and in vitro EPR methods.

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References

  • Akaike, T., Yoshida, M., Miyamoto, Y., Sato, K., Kohno, M., Sasamoto, K. Miyazaki, K. Ueda, Sand Maeda, H. (1993) Antagonistic action of imidazolineoxyl N-oxides against endothelium-derived relaxing factor/NO through a radical reaction. Biochemistry 32, 827–832.

    Article  CAS  PubMed  Google Scholar 

  • Bennett, H. F., Brown, R. D III, Keana, J. F., Koenig S. H. and Swartz. H. M. (1990) Interactions of nitroxides with plasma and blood: effect on 1/T, of water protons. Magn. Reson. Med. 14, 40–55

    CAS  Google Scholar 

  • Bland-Ward, P. A. and Moore, P. K. (1995) 7-Nitro indazole derivatives are potent inhibitors of brain, endothelium and inducible isoforms of nitric oxide synthase. Life Sci. 57, 131–135.

    Article  Google Scholar 

  • Brasch R. C., London, D. A., Wesbey, G. E., Tozer, T. N., Nitecki, D. E., Williams, R. D., Doemeny, J., Tuck, L. D. and Lallemand, D. P. (1983) Work in progress: nuclear magnetic resonance study of a paramagnetic nitroxide contrast agent for enhancement of renal structures in experimental animals. Radiology. 147, 773–779.

    CAS  PubMed  Google Scholar 

  • Chen, H. C., Sun, K. Q., Magin, R. L. and Swartz, H. M. (1990) Potential of albumin labeled with nitroxides as a contrast agent for magnetic resonance imaging and spectroscopy. Bioconjugate Chemistry 1, 32–36.

    Article  Google Scholar 

  • Fujii, H. and Berliner, L. J. (1985) One-and two-dimensional EPR imaging studies on phantoms and plant specimens. Magn. Reson. Med. 2, 275–282.

    Article  CAS  PubMed  Google Scholar 

  • Fujii, H. and Berliner, L. J. (1999) Ex-vivo detection of nitric oxide in brain tissue. Magn. Reson. Med., 42 599–602.

    Article  CAS  PubMed  Google Scholar 

  • Fujii, H., Koscielniak, J. and Berliner, L. J. (1997) Determination and characterization of nitric oxide generation in mice by in-vivo L-band EPR spectroscopy. Magn. Reson. Med. 38, 565–568.

    Article  CAS  PubMed  Google Scholar 

  • Fujii, H., Wan, X., Zhong, J., Berliner, L. J. and Yoshikawa, K (1999) In vivo imaging of spin-trapped nitric oxide in rats with septic shock: MRI spin trapping. Magn. Reson. Med. 42, 235–239.

    Article  CAS  PubMed  Google Scholar 

  • Hille, R., Olson, J. S. and Palmer, G. (1979) Spectral transitions of nitrosyl hemes during ligand binding to hemoglobin. J. Biol. Chem., 254, 12110–12120.

    CAS  PubMed  Google Scholar 

  • Hiramoto, K., Tomiyama, S. and Kikugawa, K. (1997) Appearance of electron spin resonance signals in the interaction of dithiocarbamate-Fe(II) with nitrogen dioxide and nitrite.Free Rad. Res. 27, 505–509.

    CAS  Google Scholar 

  • Knowles, R. G., Merrett, M., Salter, M. and Moncada, S. (1990) Differential induction of brain, lung and liver nitric oxide synthase by endotoxin in the rat. Biochem. J. 270, 833–836.

    CAS  PubMed Central  PubMed  Google Scholar 

  • Kojima, H., Sakurai, K., Kikuchi, K., Kawahara, S., Kirino, Y., Nagoshi, H., Hirata, Y. and Nagano, T. (1998) Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chem. Pharm. Bulletin 46, 373–375

    Article  CAS  Google Scholar 

  • Komarov, A. and Lai, C. S. (1995) Detection of nitric oxide production in mice by spin trapping electron paramagnetic resonance spectroscopy. Biochim. Biophys. Acta 1272, 29–36.

    Article  PubMed  Google Scholar 

  • Kosaka, H., Watanabe, M., Yoshihara, H., Harada, N. and Shiga, T. (1992) Detection of nitric oxide production in lipopolysaccharide-treated rats by ESR using carbon monoxide hemoglobin. Biochem. Biophys. Res. Commun. 184, 1119–1124.

    CAS  Google Scholar 

  • Kubrina, L. N., Mikoyan, V. D., Mordvintcev, P. I. and Vanin, A. F. (1993) EPR evidence for nitric oxide production from guanidino nitrogens of L-arginine. Biochim. Biophys. Acta 1176, 140–144.

    Google Scholar 

  • Kuppusamy, P., Chzhan, M., Vij, K., Shteynbuk, M., Lefer, D., Giannella E. and Zweier, J. L. (1994) Three-dimensional spectral-spatial EPR imaging of free radicals in the heart: A technique for imaging tissue metabolism and oxygenation. Proc. Natl. Acad. Sci. USA 91, 3388–3392.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Kuppusamy, P., Ohnishi, S. T., Numagami, Y., Ohnishi, T. and Zweier, J. L. (1995) Three-dimensional imaging of nitric oxide production in the rat brain subjected to ischemiahypoxia. J. Cereb. Blood Flow Metab., 15, 899–903.

    Article  CAS  PubMed  Google Scholar 

  • Kuppusamy, P., Wang, P., Samouilov, A., Zweier, J. L. (1996) Spatial mapping of nitric oxide generation in the ischemic heart using electron paramagnetic resonance imaging. Magn. Reson. Med, 36, 212–218.

    CAS  Google Scholar 

  • Lai, C. S. and Komarov, A. (1994) Spin trapping of nitric oxide produced in-vivo in septic-shock mice. FEBS Letters 345, 120–124.

    Article  CAS  PubMed  Google Scholar 

  • Lancaster, J. R. Jr., Langrehr, J. M., Bergonia, H. A., Murase, N., Simmons, R. L. and Hoffman, R. A. (1992) EPR detection of heme and nonheme iron-containing protein nitrosylation by nitric oxide during rejection of rat heart allograft. J. Biol. Chem. 184, 1119–1124.

    Google Scholar 

  • Miyajima T. and Kotake, Y. Spin trapping agent, phenyl N tert-butyl nitrone, inhibits induction of nitric oxide synthase in endotoxin-induced shock in mice. (1995) Biochem. Biophys. Res. Commun. 215, 114–121.

    Article  CAS  PubMed  Google Scholar 

  • Moncada, S., Palmer, R. M. J. and Higgs, E. A. (1991). Nitric oxide: physiology, pathophysiology, and pharmacology. Pharmacol. Rev. 43, 109–142.

    CAS  Google Scholar 

  • Mordvintcev P. Mulsch A. Busse R. and Vanin A. (1991) On-line detection of nitric oxide formation in liquid aqueous phase by electron paramagnetic resonance spectroscopy, Anal. Biochem. 199, 142–146.

    CAS  Google Scholar 

  • Morez, L. L., Norby, S. W., Cruz, L., Sweedler, J. V., Gillette, R. and Clarkson, R. B. (1998). Non-enzymatic production of nitric oxide (NO) from NO synthase inhibitors. Biochem. Biophys. Res. Commun. 253, 571–576.

    Google Scholar 

  • Nathan, C. F. (1992) Nitric oxide as a secretory product of mammalian cells. FASEB J. 6, 3051–3064.

    CAS  PubMed  Google Scholar 

  • Palmer, R. M., Rees, D. D., Ashton, D. S., Moncada, S. (1988) L-Arginine is the physiological precursor for the formation of nitric oxide in endothelium-dependent relaxation. Biochem. Biophys. Res. Commun. 153, 1251–1256.

    CAS  Google Scholar 

  • Quaresima, V., Takehara, H., Tsushima, K., Ferrari, M. and Utsumi, H. (1996) In vivo detection of mouse liver nitric oxide generation by spin trapping electron paramagnetic resonance spectroscopy. Biochem. Biophys. Res. Commun. 221, 729–734.

    CAS  Google Scholar 

  • Slane, J. M., Lai C. S. and Hyde, J. S. (1986) A proton relaxation enhancement investigation of the binding of fatty acid spin labels to human serum albumin. Magn. Reson. Med. 3, 699–706.

    CAS  Google Scholar 

  • Stuehr, D. J. and Griffith, O. W. (1992) Mammalian nitric oxide synthases. Adv. Enzymol. Relat. Areas Mol. Biol. 65, 287–346.

    CAS  PubMed  Google Scholar 

  • Stuehr, D. J. and Marietta, M. A. (1987) Induction of nitrite/nitrate synthesis in murine macrophages by BCG infection, lymphokines, or interferon gamma. J. Immunol. 139, 518–527.

    CAS  PubMed  Google Scholar 

  • Stuehr, D. J., Kwon, N. S., Nathan, C. F., Griffith, O. W., Feldmann, P. L. and Wiseman, J. (1991) N omega-hydroxyl-L-arginine is an intermediate in the biosynthesis of nitric oxide from L-arginine. J. Biol. Chem. 266, 6259–6263.

    CAS  PubMed  Google Scholar 

  • Suzuki, Y., Fujii-S, Numagami-Y, Tominaga-T, Yoshimoto-T, Yoshimura-T (1998) In vivo nitric oxide detection in the septic rat brain by electron paramagnetic resonance. Free Rad. Res. 28, 293–299.

    Article  CAS  Google Scholar 

  • Wang, Q. Z., Jacobs, J., DeLeo, J., Kruszyna, H., Kruszyna, R., Smith, R. and Wilcox, D. (1991) Nitric oxide hemoglobin in mice and rats in endotoxic shock. Life Sci. 49, 55–60.

    Article  Google Scholar 

  • Westenberger, U., Thanner, S., Ruf, H. H., Gersonde, K., Sutter, G. and Trentz, O. (1990) Formation of free radicals and nitric oxide derivative of hemoglobin in rats during shock syndrome. Free Rad. Res. Commun. 11, 167–178.

    CAS  Google Scholar 

  • Woldman, Y. Y., Khramtsov, V. V., Grigor’ev, I. A., Kirilju, I. A. and Utepbergenov, D. I. (1994) Spin trapping of nitric oxide by nitronylnitroxides: measurement of the activity of no synthase from rat cerebellum, Biochem. Biophys. Res. Commun. 202, 195–203

    CAS  Google Scholar 

  • Wong, M-L., Rettori, V., Al-Shekhlee, A., Bongiorno, P. B. and Canteros, G. (1996) Inducible nitric oxide synthase gene expression in the brain during systemic inflammation. Nature Medicine 2, 581–584

    Article  CAS  PubMed  Google Scholar 

  • Yoshimura, T., Yokoyama, H., Fujii, S., Takeyama, F., Oikawa, K. and Kamada, H. (1996). In vivo EPR detection and imaging of endogenous nitric oxide in lipopolysaccharidetreated mice. Nature Biotech. 14, 992–994.

    Article  CAS  Google Scholar 

  • Zweier, J., Wang, P., Kuppusamy, P. (1995) Direct measurement of nitric oxide generation in the ischemic heart using electron paramagnetic resonance spectroscopy. J. Biol. Chem. 270, 304–307.

    Article  CAS  PubMed  Google Scholar 

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Fujii, H., Berliner, L.J. (2003). In Vivo and in Vitro Detection of NO by EPR. In: Berliner, L.J. (eds) In Vivo EPR (ESR). Biological Magnetic Resonance, vol 18. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-0061-2_14

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  • DOI: https://doi.org/10.1007/978-1-4615-0061-2_14

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-1-4613-4906-8

  • Online ISBN: 978-1-4615-0061-2

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