Skip to main content
SpringerLink
Log in

Reactive Oxygen Species and Antioxidant Systems in Periodontal Disease

  • Chapter
  • First Online:
Studies on Periodontal Disease

Abstract

Oxidative stress induced by reactive oxygen species (ROS) is thought to play a role in the pathogenesis of various age-related diseases, including periodontitis. Many reports have suggested that dietary antioxidant deficiency results in oxidative damage at the tissue level in association with periodontal disease. Furthermore, treatment with antioxidants has been shown to prevent pathophysiological changes associated with periodontal disease and promote functional recovery in in vitro studies, animal models, and clinical trials in humans. It is therefore possible that antioxidants could be successfully used in clinical practice for the treatment or prevention of ROS-related diseases such as periodontal disease. The development of potential therapeutic antioxidants for periodontal disease requires screening using direct methods for ROS detection and appropriate animal models.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Halliwell B, Whiteman M (2004) Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean? Br J Pharmacol 142:231–255

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Sies H (1997) Oxidative stress: oxidants and antioxidants. Exp Physiol 82:291–295

    CAS  PubMed  Google Scholar 

  3. Halliwell B (2009) The wanderings of a free radical. Free Radic Biol Med 46:531–542

    Article  CAS  PubMed  Google Scholar 

  4. Nibali L, Donos N (2013) Periodontitis and redox status: a review. Curr Pharm Des 19:2687–2697

    Article  CAS  PubMed  Google Scholar 

  5. Altman LC, Baker C, Fleckman P et al (1992) Neutrophil-mediated damage to human gingival epithelial cells. J Periodontal Res 27:70–79

    Article  CAS  PubMed  Google Scholar 

  6. Liu RK, Cao CF, Meng HX et al (2001) Polymorphonuclear neutrophils and their mediators in gingival tissues from generalized aggressive periodontitis. J Periodontol 72:1545–1553

    Article  CAS  PubMed  Google Scholar 

  7. Battino M, Bullon P, Wilson M et al (1999) Oxidative injury and inflammatory periodontal diseases: the challenge of anti-oxidants to free radicals and reactive oxygen species. Crit Rev Oral Biol Med 10:458–476

    Article  CAS  PubMed  Google Scholar 

  8. Lee MC (2013) Assessment of oxidative stress and antioxidant property using electron spin resonance (ESR) spectroscopy. J Clin Biochem Nutr 52:1–8

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  9. Sugiyama S, Takahashi SS, Tokutomi FA et al (2012) Gingival vascular functions are altered in type 2 diabetes mellitus model and/or periodontitis model. J Clin Biochem Nutr 51:108–113

    Article  PubMed Central  PubMed  Google Scholar 

  10. Yoshino F, Yoshida A, Wada-Takahashi S et al (2012) Assessments of salivary antioxidant activity using electron spin resonance spectroscopy. Arch Oral Biol 57:654–662

    Article  CAS  PubMed  Google Scholar 

  11. Fridovich I (1995) Superoxide radical and superoxide dismutases. Annu Rev Biochem 64:97–112

    Article  CAS  PubMed  Google Scholar 

  12. Huie RE, Padmaja S (1993) The reaction of no with superoxide. Free Radic Res 18:195–199

    Article  CAS  Google Scholar 

  13. Massey V (1994) Activation of molecular oxygen by flavins and flavoproteins. J Biol Chem 269:22459–22462

    CAS  PubMed  Google Scholar 

  14. Halliwell B, Gutteridge JMC (eds) (2007) Free radicals in biology and medicine, 4th edn. Oxford University Press, Oxford

    Google Scholar 

  15. Dikalov S, Griendling KK, Harrison DG (2007) Measurement of reactive oxygen species in cardiovascular studies. Hypertension 49:717–727

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  16. Serrander L, Jaquet V, Bedard K et al (2007) NOX5 is expressed at the plasma membrane and generates superoxide in response to protein kinase C activation. Biochimie 89:1159–1167

    Article  CAS  PubMed  Google Scholar 

  17. Frey RS, Ushio-Fukai M, Malik AB (2009) NADPH oxidase-dependent signaling in endothelial cells: role in physiology and pathophysiology. Antioxid Redox Signal 11:791–810

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  18. Gongora MC, Qin Z, Laude K et al (2006) Role of extracellular superoxide dismutase in hypertension. Hypertension 48:473–481

    Article  CAS  PubMed  Google Scholar 

  19. Hawkins BJ, Madesh M, Kirkpatrick CJ et al (2007) Superoxide flux in endothelial cells via the chloride channel-3 mediates intracellular signaling. Mol Biol Cell 18:2002–2012

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  20. Giannopoulou C, Krause KH, Muller F (2008) The NADPH oxidase NOX2 plays a role in periodontal pathologies. Semin Immunopathol 30:273–278

    Article  CAS  PubMed  Google Scholar 

  21. Cai X, Li C, Du G et al (2008) Protective effects of baicalin on ligature-induced periodontitis in rats. J Periodontal Res 43:14–21

    CAS  PubMed  Google Scholar 

  22. Miller FJ Jr, Filali M, Huss GJ et al (2007) Cytokine activation of nuclear factor kappa B in vascular smooth muscle cells requires signaling endosomes containing Nox1 and ClC-3. Circ Res 101:663–671

    Article  CAS  PubMed  Google Scholar 

  23. Hilenski LL, Clempus RE, Quinn MT et al (2004) Distinct subcellular localizations of Nox1 and Nox4 in vascular smooth muscle cells. Arterioscler Thromb Vasc Biol 24:677–683

    Article  CAS  PubMed  Google Scholar 

  24. Chamulitrat W, Schmidt R, Tomakidi P et al (2003) Association of gp91phox homolog Nox1 with anchorage-independent growth and MAP kinase-activation of transformed human keratinocytes. Oncogene 22:6045–6053

    Article  CAS  PubMed  Google Scholar 

  25. Nakano Y, Banfi B, Jesaitis AJ et al (2007) Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Biochem J 403:97–108

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  26. Kuo LY, Hwang GY, Yang SL et al (2004) Inactivation of Bacillus stearothermophilus leucine aminopeptidase II by hydrogen peroxide and site-directed mutagenesis of methionine residues on the enzyme. Protein J 23:295–302

    Article  CAS  PubMed  Google Scholar 

  27. Stadtman ER, Berlett BS (1997) Reactive oxygen-mediated protein oxidation in aging and disease. Chem Res Toxicol 10:485–494

    Article  CAS  PubMed  Google Scholar 

  28. Kang LS, Reyes RA, Muller-Delp JM (2009) Aging impairs flow-induced dilation in coronary arterioles: role of NO and H2O2. Am J Physiol Heart Circ Physiol 297:H1087–H1095

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  29. Thengchaisri N, Kuo L (2003) Hydrogen peroxide induces endothelium-dependent and -independent coronary arteriolar dilation: role of cyclooxygenase and potassium channels. Am J Physiol Heart Circ Physiol 285:H2255–H2263

    CAS  PubMed  Google Scholar 

  30. Csordas G, Hajnoczky G (2009) SR/ER-mitochondrial local communication: calcium and ROS. Biochim Biophys Acta 1787:1352–1362

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  31. Muller G, Morawietz H (2009) NAD(P)H oxidase and endothelial dysfunction. Horm Metab Res 41:152–158

    Article  CAS  PubMed  Google Scholar 

  32. Storz P (2006) Reactive oxygen species-mediated mitochondria-to-nucleus signaling: a key to aging and radical-caused diseases. Sci STKE 2006:re3

    PubMed  Google Scholar 

  33. Hecquet CM, Ahmmed GU, Vogel SM et al (2008) Role of TRPM2 channel in mediating H2O2-induced Ca2+ entry and endothelial hyperpermeability. Circ Res 102:347–355

    Article  CAS  PubMed  Google Scholar 

  34. Wolin MS, Gupte SA, Oeckler RA (2002) Superoxide in the vascular system. J Vasc Res 39:191–207

    Article  CAS  PubMed  Google Scholar 

  35. Lu F (2007) Reactive oxygen species in cancer, too much or too little? Med Hypotheses 69:1293–1298

    Article  CAS  PubMed  Google Scholar 

  36. Vincent A, Crozatier M (2010) Neither too much nor too little: reactive oxygen species levels regulate Drosophila hematopoiesis. J Mol Cell Biol 2:74–75

    Article  CAS  PubMed  Google Scholar 

  37. Johansen JS, Harris AK, Rychly DJ et al (2005) Oxidative stress and the use of antioxidants in diabetes: linking basic science to clinical practice. Cardiovasc Diabetol 4:5

    Article  PubMed Central  PubMed  Google Scholar 

  38. Rhee SG, Bae YS, Lee SR et al (2000) Hydrogen peroxide: a key messenger that modulates protein phosphorylation through cysteine oxidation. Sci STKE 2000:pe1

    CAS  PubMed  Google Scholar 

  39. Ventura A, Pelicci PG (2002) Semaphorins: green light for redox signaling? Sci STKE 2002:pe44

    PubMed  Google Scholar 

  40. Niki E (2010) Assessment of antioxidant capacity in vitro and in vivo. Free Radic Biol Med 49:503–515

    Article  CAS  PubMed  Google Scholar 

  41. Seymour GJ, Whyte GJ, Powell RN (1986) Chemiluminescence in the assessment of polymorphonuclear leukocyte function in chronic inflammatory periodontal disease. J Oral Pathol 15:125–131

    Article  CAS  PubMed  Google Scholar 

  42. Tsai CC, Chen HS, Chen SL et al (2005) Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis. J Periodontal Res 40:378–384

    Article  CAS  PubMed  Google Scholar 

  43. Sawamoto Y, Sugano N, Tanaka H et al (2005) Detection of periodontopathic bacteria and an oxidative stress marker in saliva from periodontitis patients. Oral Microbiol Immunol 20:216–220

    Article  CAS  PubMed  Google Scholar 

  44. Wolfram RM, Budinsky AC, Eder A et al (2006) Salivary isoprostanes indicate increased oxidation injury in periodontitis with additional tobacco abuse. Biofactors 28:21–31

    Article  CAS  PubMed  Google Scholar 

  45. Tomofuji T, Ekuni D, Sanbe T et al (2009) Effects of vitamin C intake on gingival oxidative stress in rat periodontitis. Free Radic Biol Med 46:163–168

    Article  CAS  PubMed  Google Scholar 

  46. Ekuni D, Tomofuji T, Sanbe T et al (2009) Periodontitis-induced lipid peroxidation in rat descending aorta is involved in the initiation of atherosclerosis. J Periodontal Res 44:434–442

    Article  CAS  PubMed  Google Scholar 

  47. Galli C, Passeri G, Macaluso GM (2011) FoxOs, Wnts and oxidative stress-induced bone loss: new players in the periodontitis arena? J Periodontal Res 46:397–406

    Article  CAS  PubMed  Google Scholar 

  48. Akalin FA, Toklu E, Renda N (2005) Analysis of superoxide dismutase activity levels in gingiva and gingival crevicular fluid in patients with chronic periodontitis and periodontally healthy controls. J Clin Periodontol 32:238–243

    Article  CAS  PubMed  Google Scholar 

  49. Tonguc MO, Ozturk O, Sutcu R et al (2011) The impact of smoking status on antioxidant enzyme activity and malondialdehyde levels in chronic periodontitis. J Periodontol 82:1320–1328

    Article  CAS  PubMed  Google Scholar 

  50. Amarasena N, Ogawa H, Yoshihara A et al (2005) Serum vitamin C-periodontal relationship in community-dwelling elderly Japanese. J Clin Periodontol 32:93–97

    Article  CAS  PubMed  Google Scholar 

  51. Ekuni D, Tomofuji T, Sanbe T et al (2009) Vitamin C intake attenuates the degree of experimental atherosclerosis induced by periodontitis in the rat by decreasing oxidative stress. Arch Oral Biol 54:495–502

    Article  CAS  PubMed  Google Scholar 

  52. Singh N, Narula SC, Sharma RK et al (2013) Vitamin E supplementation, superoxide dismutase status and outcome of scaling and root planing in chronic periodontitis patients: a randomized clinical trial. J Periodontol DOI:10.1902/job2013.120727:1-10

    Google Scholar 

  53. de Carvalho Rde S, de Souza CM, Neves JC et al (2013) Vitamin E does not prevent bone loss and induced anxiety in rats with ligature-induced periodontitis. Arch Oral Biol 58:50–58

    Article  PubMed  Google Scholar 

  54. Hirasawa M, Takada K, Makimura M et al (2002) Improvement of periodontal status by green tea catechin using a local delivery system: a clinical pilot study. J Periodontal Res 37:433–438

    Article  CAS  PubMed  Google Scholar 

  55. Di Paola R, Mazzon E, Zito D et al (2005) Effects of Tempol, a membrane-permeable radical scavenger, in a rodent model periodontitis. J Clin Periodontol 32:1062–1068

    Article  PubMed  Google Scholar 

  56. Kim do Y, Jun JH, Lee HL et al (2007) N-acetylcysteine prevents LPS-induced pro-inflammatory cytokines and MMP2 production in gingival fibroblasts. Arch Pharm Res 30:1283–1292

    Article  PubMed  Google Scholar 

  57. Cheng WC, Huang RY, Chiang CY et al (2010) Ameliorative effect of quercetin on the destruction caused by experimental periodontitis in rats. J Periodontal Res 45:788–795

    Article  CAS  PubMed  Google Scholar 

  58. Govindaraj J, Emmadi P, Deepalakshmi et al (2010) Protective effect of proanthocyanidins on endotoxin induced experimental periodontitis in rats. Indian J Exp Biol 48:133–142

    CAS  PubMed  Google Scholar 

  59. Kasuyama K, Tomofuji T, Ekuni D et al (2011) Hydrogen-rich water attenuates experimental periodontitis in a rat model. J Clin Periodontol 38:1085–1090

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by a Grant-in-Aid for Scientific Research (no. 18592149 to M.L., no. 19592371 to T.K. & M.L., no. 23593049 to T.K., no. 23660047 to M.L.) from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

Author information

Authors and Affiliations

  1. Yokosuka-Shonan Disaster Health Emergency Research Center & ESR Laboratories, Kanagawa Dental University, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan

    Masaichi-Chang-il Lee

Authors
  1. Masaichi-Chang-il Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Masaichi-Chang-il Lee .

Editor information

Editors and Affiliations

  1. Department of Preventive Dentistry, Okayama University Graduate School of Medicine and Pharmaceutical Sciences, Okayama, Japan

    Daisuke Ekuni

  2. Dept of Science and Clinical Specialty Odontostomatology & Biochemistry, Polytechnical University of Marche Faculty of Medicine, Ancona, Italy

    Maurizio Battino

  3. Department of Preventive Dentistry, Okayama Univ Graduate School of Medicine Dentistry and Pharmaceutical Sciences, Okayama, Japan

    Takaaki Tomofuji

  4. Dept of Oral Biological & Medical Scienc Laboratory of Periodontal Biology, University of British Columbia Faculty of Dentistry, Vancouver, British Columbia, Canada

    Edward E. Putnins

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this chapter

Cite this chapter

Lee, MCi. (2014). Reactive Oxygen Species and Antioxidant Systems in Periodontal Disease. In: Ekuni, D., Battino, M., Tomofuji, T., Putnins, E. (eds) Studies on Periodontal Disease. Oxidative Stress in Applied Basic Research and Clinical Practice. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4614-9557-4_1

Download citation

Publish with us

Policies and ethics

Search

Navigation