Reconstruction from Microscopic Projections with Defocus-Gradient and Attenuation Effects

Part of the Applied and Numerical Harmonic Analysis book series (ANHA)


We discuss and illustrate defocus-gradient and attenuation effects that are part of the image formation models of microscopy of biological specimens. We demonstrate how they affect the projection data and in turn the 3D reconstructions. Biologically meaningful results can be obtained ignoring both of these effects, but using image processing techniques to incorporate corrections for them into reconstruction methods provides more accurate reconstructions, with potential for creating higher-resolution models of the biological specimens.


Reconstruction Method Projection Data Projection Image Attenuation Effect Forward Problem 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The work presented here is currently supported by the National Science Foundation award number DMS-1114901.

The authors would like to thank Joaquín Otón, José-María Carazo, Carlos Óscar Sánchez Sorzano, and Roberto Marabini for helpful discussions on microscopy and Roberto Marabini and Joachim Frank for comments on this manuscript.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Computer Science, The Graduate CenterCity University of New YorkNew YorkUSA

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