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Nanotoxicology pp 177–199Cite as

Genetic Studies on the Effects of Nanomaterials

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Part of the book series: Nanomedicine and Nanotoxicology ((NANOMED))

Abstract

The aim of this chapter is to present some of the principal methodologies used to study the effects of toxic substances on DNA, which can be used to analyze aspects of nanomaterial toxicity. It is not the intention here to undertake an in-depth survey of the topic, but rather to highlight the techniques that can be used in nanotoxicity studies. The available methods for evaluating effects on DNA include genotoxicity tests such as (1) the Allium cepa chromosome aberration test, (2) the comet analysis, (3) the micronucleus test, and (4) the cytogenetic analysis. In addition, this chapter also described the methodologies for analysis of gene expression that can be applied to the effects of nanomaterials. A description of the characteristics of each method is provided, together with results of selected studies that have evaluated the effects of nanomaterials, and a critical discussion of the main advantages and disadvantages of each technique. The chapter also highlights the challenges and future perspectives for studies of the effects of nanostructured materials on DNA.

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Acknowledgements

Supported by FAPESP, CNPq, Brazilian Network on Nanotoxicology (MCTI/CNPq), INOMAT (CNPq), NanoBioss (CMTI/CNPq).

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Authors and Affiliations

  1. Department of Biotechnology, University of Sorocaba, Rodovia Raposo Tavares S/N, Km 92,5, CEP 18023-000, Sorocaba, São Paulo, Brazil

    Renata de Lima

  2. Universidade Federal de São Carlos, UFSCar, Sorocaba, São Paulo, Brazil

    Renata de Lima

  3. Department of Environmental Engineering, São Paulo State University - UNESP, Av. Três de Março, 511, Alto da Boa Vista, 18087-180, Sorocaba, São Paulo, Brazil

    Leonardo Fernandes Fraceto

Authors
  1. Renata de Lima
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  2. Leonardo Fernandes Fraceto
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Correspondence to Renata de Lima .

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Editors and Affiliations

  1. Institute of Chemistry, Universidade Estadual de Campinas—UNICAMP, Campinas, São Paulo, Brazil

    Nelson Durán

  2. Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil

    Silvia S. Guterres

  3. Institute of Chemistry, Universidade Estadual de Campinas—UNICAMP, Campinas, São Paulo, Brazil

    Oswaldo L. Alves

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de Lima, R., Fraceto, L.F. (2014). Genetic Studies on the Effects of Nanomaterials. In: Durán, N., Guterres, S., Alves, O. (eds) Nanotoxicology. Nanomedicine and Nanotoxicology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8993-1_8

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