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Using Synchrotron FTIR and Confocal Cryomicroscopy to Explore Mechanisms of Cold Acclimation and Freezing Resistance Using a Single Cell Layer of Allium fistulosum L

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Book cover Plant and Microbe Adaptations to Cold in a Changing World

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Abstract

Synchrotron-based Fourier Transform Infrared (FTIR) microspectroscopy is a powerful tool for nondestructively highlighting spatial distribution of candidate chemical components and properties on a single plant cell basis. We have used this technique to localize changes after cold acclimation in a new plant system based on a cold hardy perennial onion, Allium fistulosum L. Confocal cryomicroscopy combined with membrane-specific fluorescent dyes in the A. fistulosum L. model system also represents a powerful technique to observe in-situ cellular responses to freezing and thawing. This cold hardy onion system enables both techniques to be used nondestructively with observations acquired on a single cell basis. This chapter provides a review of techniques and illustrates their potential based on our experiments.

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Acknowledgments

The research performed in Iwate University was supported by Grant-in-Aid for Scientific Research on Innovative Areas (#22120003 to M. U.), Grant-in-Aid for Young Scientists (B) (#22780288 to Y. K.), Grant-in-Aid for Scientific Research (B) (#24370018 to M. U.), and “Live imaging of plant cells under freezing,” Adaptable and Seamless Technology Transfer Program through Target-driven R&D, JST (to Y. K.). The research described in this chapter was performed at the Mid-IR beamline of the Canadian Light Source, which is supported by NSERC, NRC, CIHR, and the University of Saskatchewan. Tim May and Xia Liu are gratefully acknowledged for their assistance.

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Authors and Affiliations

  1. Department of Plant Sciences, University of Saskatchewan, Saskatoon, SK, Canada

    Karen Tanino & Jun Liu

  2. Cryobiofrontier Research Center, Faculty of Agriculture, Iwate University, Morioka, Japan

    Shion Kobayashi, Yukio Kawamura & Matsuo Uemura

  3. Canadian Light Source, Saskatoon, SK, Canada

    Ferenc Borondics

Authors
  1. Karen Tanino
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  2. Jun Liu
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  3. Shion Kobayashi
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  4. Yukio Kawamura
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  5. Ferenc Borondics
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  6. Matsuo Uemura
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Corresponding author

Correspondence to Karen Tanino .

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Editors and Affiliations

  1. Crop Breeding Research Division, NARO Hokkaido Agricultural Research Center, Sapporo, Japan

    Ryozo Imai

  2. Crop Breeding Research Division, NARO Hokkaido Agricultural Research Center, Sapporo, Japan

    Midori Yoshida

  3. Silviculture & Forest Ecology Studies, Hokkaido University Graduate School of Agriculture, Sapporo, Japan

    Naoyuki Matsumoto

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Tanino, K., Liu, J., Kobayashi, S., Kawamura, Y., Borondics, F., Uemura, M. (2013). Using Synchrotron FTIR and Confocal Cryomicroscopy to Explore Mechanisms of Cold Acclimation and Freezing Resistance Using a Single Cell Layer of Allium fistulosum L. In: Imai, R., Yoshida, M., Matsumoto, N. (eds) Plant and Microbe Adaptations to Cold in a Changing World. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8253-6_14

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