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Hawaii’s Transgenic Papaya Story 1978–2012: A Personal Account

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Part of the book series: Plant Genetics and Genomics: Crops and Models ((PGG,volume 10))

Abstract

In May 1998, papaya ring spot virus (PRSV)-resistant transgenic “Rainbow” and “SunUp” papaya were released to growers and helped save the papaya industry in Hawaii. This review provides a personal account of the Hawaii transgenic papaya story from 1978 to 2012. It traces the general technical development of the papaya, but more importantly, it provides a personal account of the events in the story. These events include the rationale to proactively develop control methods in the event the PRSV would enter Puna, where 95 % of Hawaii’s papaya were being grown in 1992; the formation and motivation of the research team; the coinciding of the transgenic papaya development with the invasion of Puna by PRSV; the deregulation and commercialization of the transgenic papaya in the US; and the long road to its deregulation and commercialization in Japan. And it describes activities in the “red zone” of translational biotechnology.

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References

  • Camp III SG (2003) Virus resistant transgenic papaya in Hawaii: a case for technology transfer to lesser developed countries. In: OECD/USAID/ARS conference, Hilo, 22–24 October 2003

    Google Scholar 

  • Clark MF, Adams AN (1977) Characteristics of the microplate method for enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virol 34:475–483

    Article  PubMed  CAS  Google Scholar 

  • Fermin G, Tennant P, Gonsalves C, Lee D, Gonsalves D (2004) Comparative development and impact of transgenic papayas in Hawaii, Jamaica, and Venezuela. In: Pena L (ed) Transgenic plants: methods and protocols. The Humana Press Inc, Totowa, pp 399–430

    Chapter  Google Scholar 

  • Fermin G, Keith RC, Suzuki JY, Ferreira S, Gaskill DA, Pitz KY, Manshardt RM, Gonsalves D, Tripathi S (2011) Allergenicity assessment of the papaya ringspot virus coat protein expressed in transgenic Rainbow papaya. J Agric Food Chem 59(18):10006–10012

    Article  PubMed  CAS  Google Scholar 

  • Ferreira SA, Mau RFL (1994) A synopsis of the phased plan for eradicating papaya ringspot disease from the Puna district, Island of Hawaii. In: Proceedings of the 30th annual Hawaii Papaya Industry Association conference, Kihei, 23–24 September 1994, pp 7–9

    Google Scholar 

  • Ferreira SA, Pitz KY, Manshardt R, Zee F, Fitch M, Gonsalves D (2002) Virus coat protein transgenic papaya provides practical control of papaya ringspot virus in Hawaii. Plant Disease 86:101–105

    Article  Google Scholar 

  • Fitch M, Manshardt R (1990) Somatic embryogenesis and plant regeneration from immature zygotic embryos of papaya (Carica papaya L.). Plant Cell Rep 9:320–324

    CAS  Google Scholar 

  • Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC (1990) Stable transformation of papaya via microprojectile bombardment. Plant Cell Rep 9(4):189–194

    Article  CAS  Google Scholar 

  • Fitch MMM, Manshardt RM, Gonsalves D, Slightom JL, Sanford JC (1992) Virus resistant papaya derived from tissues bombarded with the coat protein gene of papaya ringspot virus. Biotechnology 10:1466–1472

    Article  CAS  Google Scholar 

  • Fuchs M, Gonsalves D (1995) Resistance of transgenic hybrid squash ZW-20 expressing the coat protein genes of zucchini yellow mosaic virus and watermelon mosaic virus 2 to mixed infections by both potyviruses. Biotechnology 13(13):1466–1473

    CAS  Google Scholar 

  • Gonsalves D (1998) Control of papaya ringspot virus in papaya: a case study. Annu Rev Phytopathol 36:415–437

    Article  PubMed  CAS  Google Scholar 

  • Gonsalves D (2006) Transgenic papaya: development, release, impact, and challenges. Adv Virus Res 67:317–354

    Article  PubMed  CAS  Google Scholar 

  • Gonsalves D, Ferreira S (2003) Transgenic papaya: a case for managing risks of papaya ringspot virus in Hawaii. Plant Health Progress. doi:10.1094/PHP-2003-1113-03-RV

    Google Scholar 

  • Gonsalves D, Garnsey SM (1989) Cross protection techniques for control of plant virus diseases in the tropics. Plant Disease 73:592–597

    Article  Google Scholar 

  • Gonsalves D, Ishii M (1980) Purification and serology of papaya ringspot virus. Phytopathology 70:1028–1032

    Article  CAS  Google Scholar 

  • Gonsalves D, Fuchs M, Klas F, Tennant P (1994) Field assessment of risks when using transgenic papayas, cucurbits, and tomatoes expressing viral coat protein genes. In: Jones DD (ed) Proceedings of 3rd international symposium on the biosafety results of field tests of genetically modified plants and microorganisms. University of California, Division of Agriculture and Natural Sciences, Oakland, 13–16 November 1994, pp 117–127

    Google Scholar 

  • Gonsalves D, Ferreira S, Manshardt R, Fitch M, Slightom J (1998) Transgenic virus resistant papaya: new hope for control of papaya ringspot virus in Hawaii. APSnet feature story for September 1998 on world wide web. http://www.apsnet.org/education/feature/papaya/Top.htm

  • Gonsalves C, Lee D, Gonsalves D (2004a) Transgenic virus resistant papaya: The Hawaiian “Rainbow” was rapidly adopted by farmers and is of major importance in Hawaii today. Online. APSnet Feature, American Phytopathological Society (August–September). http://www.apsnet.org/online/feature/rainbow

  • Gonsalves D, Gonsalves C, Ferreira S, Pitz K, Fitch M, Manshardt R, Slightom J (2004b) Transgenic virus resistant papaya: from hope to reality for controlling of papaya ringspot virus in Hawaii. Online. APSnet Feature, American Phytopathological Society (August–September). http://www.apsnet.org/online/feature/ringspot/ (July 2004)

  • Gonsalves D, Vegas A, Prasartsee V, Drew R, Suzuk J, Tripathi S (2006) Developing papaya to control papaya ringspot virus by transgenic resistance, intergeneric hybridization, and tolerance breeding. Plant Breed Rev 26:35–78

    CAS  Google Scholar 

  • Gonsalves C, Lee DR, Gonsalves D (2007) The adoption of genetically modified papaya in Hawaii and its implications for developing countries. J Dev Stud 43:177–191

    Article  Google Scholar 

  • Gonsalves D, Ferreira S, Suzuki J, Tripathi S (2008a) Papaya. In: Kole C, Hall TC (eds) A compendium of transgenic crop plants: tropical and subtropical fruits and nuts, vol 5. Blackwell, Oxford, pp 131–162

    Google Scholar 

  • Gonsalves D, Suzuki J, Tripathi S, Ferreira S (2008b) Papaya ringspot virus (Potyviridae). In: Mahy B, Van Regenmortel M (eds) Encyclopedia of virology, 5 vols (vol 4), 3rd edn. Elsevier, Oxford, pp 1–8

    Google Scholar 

  • Gonsalves D, Gonsalves C, Carr J, Tripathi S, Matsumoto T, Suzuki J, Ferreira S, Pitz K (2012) Assaying for pollen drift from transgenic “Rainbow” to nontransgenic “Kapoho” papaya under commercial and experimental field conditions in Hawaii. Trop Plant Biol 5(1):53–160

    Google Scholar 

  • Isherwood MOJ (1992) Papaya ringspot virus in Puna: chronology of events and eradication program update. In: Proceedings of the 28th annual Hawaii Papaya Industry Association conference, 25–26 September 1992, Honolulu, pp 7–9

    Google Scholar 

  • Isherwood MOJ (1994) Status of papaya ringspot virus program. In: Proceedings of the 30th annual Hawaii Papaya Industry Association conference, 23–24 September 1994, Kihei, pp 1–3

    Google Scholar 

  • Lius S, Manshardt RM, Fitch MMM, Slightom JL, Sanford JC, Gonsalves D (1997) Pathogen-derived resistance provides papaya with effective protection against papaya ringspot virus. Mol Breed 3:161–168

    Article  Google Scholar 

  • Manshardt RM (1998) “UH Rainbow” papaya. University of Hawaii College of Tropical Agriculture and Human Resources Germplasm G-1, 2 pp

    Google Scholar 

  • Mau RFL, Gonsalves D, Bautista R (1989) Proceedings of the 25th annual Papaya Industry Association conference, Hilo

    Google Scholar 

  • Ming R, Hou S, Feng Y, Yu Q, Dionne-Laporte A, Saw JH et al (2008) A draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus). Nature 452:991–996

    Article  PubMed  CAS  Google Scholar 

  • Powell-Abel P, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Beachy RN (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232(4751):738–743

    Article  Google Scholar 

  • Quemada H, L'Hostis B, Gonsalves D, Reardon IM, Heinrikson R, Hieber EL, Sieu LC, Slightom JL (1990) The nucleotide sequences of the 3′-terminal regions of papaya ringspot virus strains w and p. J Gen Virol 71(1):203–210

    Article  PubMed  CAS  Google Scholar 

  • Sanford JC, Johnston SA (1985) The concept of parasite-derived resistance – deriving resistance genes from the parasite’s own genome. J Theor Biol 113:395–405

    Article  Google Scholar 

  • Sato S (2011) Japan approved GM papaya. USDA Foreign Agricultural Service Gain Report number JA1048

    Google Scholar 

  • Souza MT Jr, Tennant PF, Gonsalves D (2005) Influence of coat protein transgene copy number on resistance in transgenic line 63–1 against papaya ringspot virus isolates. Hortscience 40(7):2083–2087

    Google Scholar 

  • Strating A (1996) Availability of determination of nonregulated status for papaya lines genetically engineered for virus resistance. Fed Regist 61(48663)

    Google Scholar 

  • Suzuki JY, Tripathi S, Fermin GA, Jan F-J, Hou S, Saw JH, Ackerman CM, Yu Q, Schatz MC, Pitz KY, Yepes M, Fitch MMM, Slightom JL, Ferreira SA, Salzberg SL, Alam M, Ming R, Moore PH, Gonsalves D (2008) Characterization of insertion sites in Rainbow papaya, the first commercialized transgenic papaya fruit crop. Trop Plant Biol 1:293–309

    Article  CAS  Google Scholar 

  • Tennant PF, Gonsalves C, Ling KS, Fitch M, Manshardt R, Slightom JL, Gonsalves D (1994) Differential protection against papaya ringspot virus isolates in coat protein gene transgenic papaya and classically cross-protected papaya. Phytopathology 84(11):1359–1366

    Article  Google Scholar 

  • Tennant P, Fermin G, Fitch MM, Manshardt RM, Slightom JL, Gonsalves D (2001) Papaya ringspot virus resistance of transgenic Rainbow and SunUp is affected by gene dosage, plant development, and coat protein homology. Eur J Plant Pathol 107(6):645–653

    Article  CAS  Google Scholar 

  • Tennant PF, Souza M, Fitch MM, Manshardt RM, Slightom JL, Gonsalves D (2005) Line 63–1, a new virus-resistant transgenic papaya. Hortscience 40:1196–1199

    Google Scholar 

  • Tricoli DM, Carney KJ, Russell PF, McMaster JR, Groff DW, Hadden KC, Himmel PT, Hubbard JP, Boeshore ML, Quemada HD (1995) Field evaluation of transgenic squash containing single or multiple virus coat protein gene constructs for resistance to cucumber mosaic virus, watermelon mosaic virus 2, and zucchini yellow mosaic virus. Biotechnology 13(13):1458–1465

    CAS  Google Scholar 

  • Tripathi S, Suzuki JY, Carr JB, McQuate GT, Ferreira SA, Manshardt RM, Pitz KY, Wall MM, Gonsalves D (2011) Nutritional composition of Rainbow papaya, the first commercialized transgenic fruit crop. J Food Compos Anal 24(2):140–147

    Article  CAS  Google Scholar 

  • Yeh SD, Gonsalves D (1984) Evaluation of induced mutants of papaya ringspot virus for control by cross protection. Phytopathology 74(9):1086–1091

    Article  Google Scholar 

  • Yeh S-D, Gonsalves D (1994) Practices and perspective of control of papaya ringspot virus by cross protection. In: Harris KF (ed) Advances in disease vector research, vol 10. Springer, New York, pp 237–257

    Chapter  Google Scholar 

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Acknowledgements

I am fortunate to have been associated with many people that contributed to the papaya story. They are too many to name and I undoubtedly missed a number of them in the text of the story. I thank all of you as I complete my involvement in the Hawaii transgenic papaya story and wish good fortunes to those that will continue. I specifically thank Carol my wife who has been with me throughout this entire story. Most importantly, Rickie Deniz, a true friend that I had for such a short time, showed me how to really get things done in the “red zone.”

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Correspondence to Dennis Gonsalves .

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Gonsalves, D. (2014). Hawaii’s Transgenic Papaya Story 1978–2012: A Personal Account. In: Ming, R., Moore, P. (eds) Genetics and Genomics of Papaya. Plant Genetics and Genomics: Crops and Models, vol 10. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-8087-7_7

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