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Cancer Targeted Drug Delivery pp 635–665Cite as

In Vitro Three-Dimensional Cancer Culture Models

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Abstract

The efficacy of chemotherapy drug candidates is conventionally investigated using 2D cancer cell cultures and in vivo animal models. It is crucial to determine signaling pathways, controlling cell proliferation, metabolism, differentiation, and apoptosis functions, which are not optimal to investigate in the monolayer 2D cell culture models. Further, accurate investigation of tumor growth and therapeutic drug efficacy in murine models is challenging because of technical constraints of in vivo imaging and requires euthanizing the animals. Therefore, alternative in vitro cancer models are needed to facilitate the transition of new chemotherapeutic drug candidates from bench to clinical trials. Recent technological advances in microfabrication and bioengineering have provided tools to develop in vitro 3D cancer models that mimic natural tissue microenvironment. This chapter highlights recent developments in in vitro 3D cancer models and their applications for studying the efficacy of the chemotherapeutic drug candidates. We discuss the methods and technologies to develop 3D cancer models including embedded and overlay cell culture, suspension culture, bioprinting, hanging drop, microgravity bioreactor, and magnetic levitation. We also discuss the extracellular matrix components and synthetic scaffolds used in vitro 3D cancer models.

Waseem Asghar and Hadi Shafiee contributed equally to this work.

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Authors and Affiliations

  1. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, USA

    Waseem Asghar, Hadi Shafiee, Pu Chen, Savas Tasoglu & Sinan Guven

  2. CASE Biomanufacturing and Microfabrication Laboratory (CASE-BML), Department of Mechanical and Aerospace Engineering, Advanced Platform Technology Center, Louis Stokes Cleveland Veterans Affairs Medical Center, Case Western Reserve University, Cleveland, OH, USA

    Umut Atakan Gurkan

  3. Bio-Acoustic-MEMS in Medicine (BAMM) Laboratory, Center for Biomedical Engineering and Division of Infectious Diseases, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, MA, USA

    Utkan Demirci

  4. Harvard-MIT Health Sciences and Technology, Cambridge, MA, USA

    Utkan Demirci

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  1. Waseem Asghar
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  2. Hadi Shafiee
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  3. Pu Chen
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  4. Savas Tasoglu
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  5. Sinan Guven
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  6. Umut Atakan Gurkan
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  7. Utkan Demirci
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Correspondence to Utkan Demirci .

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Editors and Affiliations

  1. Pharmacutics & Pharmacutical Chemistry, University of Utah, Salt Lake City, Utah, USA

    You Han Bae

  2. Department of Pharmacy & Pharmacology, University of Bath, Claverton Down, Bath, United Kingdom

    Randall J. Mrsny

  3. Department of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA

    Kinam Park

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Asghar, W. et al. (2013). In Vitro Three-Dimensional Cancer Culture Models. In: Bae, Y., Mrsny, R., Park, K. (eds) Cancer Targeted Drug Delivery. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7876-8_24

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