Effects of Cholesterol-Depleting Agent Methyl-β-Cyclodextrin on the Functional State of Brain Nerve Terminals

  • Tatiana Borisova
Part of the SpringerBriefs in Neuroscience book series (BRIEFSNEUROSCI, volume 12)


Cholesterol acceptor methyl-β-cyclodextrin (MβCD) (15 mM) reduced the cholesterol content in brain nerve terminals (synaptosomes) by one quarter. The application of MβCD to the synaptosomes as well as isolated synaptic vesicles led to the gradual leakage of the protons from the vesicles, as shown by acridine orange fluorescence measurements, whereas the application of MβCD complexed with cholesterol (15:0.2) that increased the membrane cholesterol content stimulated additional vesicle acidification. It was supposed that cholesterol depletion of the plasma membrane with MβCD induced the removal of cholesterol from the membranes of synaptic vesicles resulting in immediate dissipation of synaptic vesicle proton gradient, and so could provoke redistribution of the neurotransmitter between the vesicular and cytosolic pools.


Synaptic Vesicle Acridine Orange Proton Gradient Vesicle Acidification Cholesterol Depletion 
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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Tatiana Borisova
    • 1
  1. 1.Department of Neurochemistry Palladin Institute of BiochemistryNational Academy of Sciences of UkraineKievUkraine

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