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Fluorophores and Their Labeling Procedures for Monitoring Various Biological Signals

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Methods in Cellular Imaging

Part of the book series: Methods in Physiology ((METHPHYS))

Abstract

Fluorescence microscopy occupies a unique position in the biological and biomedical sciences where fluorescence probe specificity and sensitivity can provide important information regarding the biochemical, biophysical, and structural status of cells and tissues. The continuing development of fluorescent probes, or fluorophores, in conjunction with the strong emergence over the past two decades of confocal and multiphoton microscopy (and specialized applications such as fluorescene recovery after photobleaching [FRAP], fluorescence resonance energy transfer [FRET], and fluorescence lifetime imaging [FLIM]) has been a major contributor to our understanding of dynamic processes in cells and tissue.

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Authors
  1. Ian S. Harper
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Editors and Affiliations

  1. W. M. Keck Center for Cellular Imaging, University of Virginia, Charlottesville, Virginia, USA

    Ammasi Periasamy Ph.D. (Director, Professor of Biology and Biomedical Engineering) (Director, Professor of Biology and Biomedical Engineering)

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© 2001 American Physiological Society

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Harper, I.S. (2001). Fluorophores and Their Labeling Procedures for Monitoring Various Biological Signals. In: Periasamy, A. (eds) Methods in Cellular Imaging. Methods in Physiology. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7513-2_2

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  • DOI: https://doi.org/10.1007/978-1-4614-7513-2_2

  • Publisher Name: Springer, New York, NY

  • Online ISBN: 978-1-4614-7513-2

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