Leukocyte Recruitment as Seen by Intravital Microscopy

  • Klaus Ley
Part of the Methods in Physiology Series book series (METHPHYS)

Abstract

The term leukocyte recruitment encompasses all events that bring circulating leukocytes into inflamed tissues. Recruitment is thought to proceed in a cascade-like fashion (Ley, 1989; Butcher, 1991; Springer, 1995; Fig. 16.1). This cascade has many rocks, nooks, and dead-water zones because there are parallel pathways and many decision points along the way. In inflammation, neutrophils, eosinophils, monocytes, T-lymphocytes, and even basophils and B-lymphocytes can be recruited. Intravital microscopy is limited in its ability to distinguish leukocyte subpopulations. In most tissues and most models of inflammation, the neutrophil is the most prevalent cell. Therefore, this chapter uses neutrophil recruitment as an example to illustrate the determinants, parameters, models, and physiology of leukocyte recruitment.

Keywords

Permeability Migration Ischemia Histamine Luminal 

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  • Klaus Ley

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