Abstract
The matrix-assisted laser desorption ionization (MALDI) process constitutes a complex mixture of events, involving optical and mechanical phenomena, as well as thermodynamic and physicochemical processes of phase transition and ionization. The experiment is based on irradiation of the surface of a solid sample that is a mixture of a photosensitizer material (the matrix) and analyte(s) with a short-pulse UV laser. A successful MALDI analysis involves a number of crucial steps, namely, sample preparation, UV excitation of the matrix (photosensitizer)–analyte sample and disintegration of the condensed phase, generation and separation of charges and ionization of analyte and matrix molecules, and, finally, in the analysis step, ion separation according to the mass-to-charge ratio in the mass spectrometer, and detection (Fig. 9.1).
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References
Tanaka K, Waki H, Ido Y, Akita S, Yoshida Y, Yoshida T (1998) Protein and polymer analyses up to m/z 100,000 by laser ionization time-of-flight mass spectrometry. Rapid Comunn Mass Spectrom 2:151–153
Karas M, Hillenkamp F (1998) Laser desorption ionization of proteins with molecular masses exceeding 10,000 daltons. Anal Chem 60:2299–2301
Hillenkamp F, Peter-Katalinić J (eds) (2007) MALDI MS. A practical guide to instrumentation, methods and applications. Wiley-VCH, Weinheim
Cole RB (2010) Electrospray and MALDI mass spectrometry. Fundamentals, instrumentation, practicalities, and biological applications. Wiley, London
Phipps C (ed) (2007) Laser ablation and its applications, 2nd edn. Springer, New York
Klessinger M, Michl J (1995) Excited states and photochemistry of organic molecules. VCH, New York
Turro NJ, Ramamurthy V, Scaiano JC (2010) Modern molecular photochemistry of organic molecules. University Science Books, Sausalito
Cohen-Tannoudji C, Diu B, Laloė F (1997) Quantum mechanics. Wiley, New York
Atkins PW (1986) Physical chemistry. Oxford University Press, Oxford
Birks JB (1975) Organic molecular photophysics vols I and II. Wiley, Chichester
Simons JP (1971) Photochemistry and spectroscopy. Wiley-Interscience, London
Valeur B (2002) Molecular fluorescence. Wiley-VCH, Weinheim
Zander C, Enderlein J, Keller RA (2002) Single molecule detection in solution. Wiley-VCH, Berlin
Jablonski A (1935) Über den mechanismus der photolumineszens von farbestoffphosphoren. Z Phys 94:38–46
Lakowicz JR (ed) (1991) Topics in fluorescence spectroscopy, vol 1 and 2. Plenum, New York
Cheung HC (1991) Resonance energy transfer. In: Lakowicz JR (ed) Topics in fluorescence spectroscopy, vol 2. Plenum, New York
Chiang CK, Chen WT, Chang HT (2011) Nanoparticle-based mass spectrometry for the analysis of biomolecules. Chem Soc Rev 40:1269–1281
Lane N (2001) The grand challenges of nanotechnology. J Nanopart Res 3:95–103
Karplus M, Porter RN (1970) Atoms and molecules; an introduction for students of physical chemistry. W. A. Benjamin, New York
Harrison WA (1980) Electronic structure and the properties of solids. Freeman, San Francisco
Brus LE (1984) Electron–electron and electron–hole interactions in small semiconductors crystallites: the size dependence of the lowest excited electronic state. J Chem Phys 80:4403–4409
Gaponenko SV (1998) Optical properties of semiconductor nanocrystals. Cambridge University Press, Cambridge
Harrison WA (1989) Electronic structure and the properties of solids: the physics of the chemical bond. Dover Publications, Dover
Klimov VI, McBranch DW, Leatherdale CA, Bawendi MG (1999) Electron and hole relaxation pathways in semiconductor quantum dots. Phys Rev B 60:13740–13749
Vertes A (2007) Laser-mater interaction in novel regimes. In: Phipps C (ed) Laser ablation and its applications. Springer, New York
Schmid G (ed) (2004) Nanoparticles: from theory to application. Wiley-VCH, Weinheim
Rosencwaig A, Gersho A (1976) Theory of photoacoustic effect in solids. J Appl Phys 47:64–69
Phipps C (ed) (2006) Laser ablation and its applications, 1st edn. Springer, New York
Bäuerle D (2011) Laser processing and chemistry, 4th edn. Springer, New York
Braslavsky SE, Heibel GH (1992) Time-resolved photothermal and photoacoustic methods applied to photoinduced processes in solution. Chem Rev 92:1381–1410, and references therein
Landau LD, Lifschitz EM (1959) Fluid mechanics. Pergamon, Oxford, Chap VIII
Van Haver P, Viaene L, Van der Auweraer M, De Schryver FC (1992) References for laser-induced opto-acoustic spectroscopy using UV excitation. J Photochem Photobiol A Chem 63:265–277
Murgida DH, Erra-Balsells R, Bilmes GM (1996) New photocalorimetric references for UV excitation. Chem Phys Lett 250:198–202
Mesaros M, Tarzi OI, Erra-Balsells R, Bilmes GM (2006) The photophysics of some UV-MALDI matrices studied by using spectroscopic, photoacoustic and luminescence techniques. Chem Phys Lett 426:334–340
Petroselli G, Gara PD, Bilmes GM, Erra-Balsells R (2012) Photoacoustic and luminescence characterization of nitrogen heterocyclic aromatic UV-MALDI matrices in solution. Photochem Photobiol Sci 11:1062–1068
Vogel A, Venugopalan V (2003) Mechanisms of pulsed laser ablation of biological tissues. Chem Rev 103:577–644
Vèkey K, Telekes A, Vertes A (eds) (2008) Medical applications of mass spectrometry. Elsevier, Amsterdam
Colombini MP, Modugno F (eds) (1988) Organic mass spectrometry in art and archaeology. Wiley, Florencia
Georgiou S, Hillenkamp F (eds) (2003) Introduction: laser ablation of molecular substrates. Chem Rev 103:317–319
Dreisewerd K (2003) The desorption process in MALDI. Chem Rev 103:395–425
Georgiou S, Koubenakis A (2003) Laser-induced material ejection from model molecular solids and liquids: mechanisms, implications, and applications. Chem Rev 103:349–393
Zhigilei LV, Leveugle E, Garrison BJ, Yingling YG, Zeifman MI (2003) Computer simulation of laser ablation of molecular substrates. Chem Rev 103:321–347
Paltauf G, Dyer PE (2003) Photomechanical processes and effects in ablation. Chem Rev 103:487–518
Royer D, Dieulesaint E (2000) Elastic waves in solids I, II. Springer, Berlin
Itzkan I, Albagli D, Dark M, Perelman L, von Rosenberg C, Feld MS (1995) The thermoelastic basis of short pulsed laser ablation of biological tissue. Proc Natl Acad Sci U S A 92:1960–1964
Albagli D, Dark M, von Rosenberg C, Perelman L, Itzkan I, Feld M (1994) Laser‐induced thermoelastic deformation: a three‐dimensional solution and its application to the ablation of biological tissue. Med Phys 21:1323–1332
Albagli D, Dark M, Perelman LT, von Rosenberg C, Itzkan I, Feld MS (1994) Photomechanical basis of laser ablation of biological tissue. Opt Lett 19:1684–1686
Koulikov SG, Dlott DD (2001) Ultrafast microscopy of laser ablation of refractory materials: ultra low threshold stress-induced ablation. J Photochem Photobiol A Chem 145:183–194
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Erra-Balsells, R. (2013). MALDI: A Very Useful UV Light-Induced Process…That Still Remains Quite Obscure. In: Hiraoka, K. (eds) Fundamentals of Mass Spectrometry. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-7233-9_9
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