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Quantitative SNP Array and Real-Time PCR-Based Human Preimplantation Embryo Aneuploidy Screening

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Human Gametes and Preimplantation Embryos

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Abstract

Chromosome imbalance (aneuploidy) is the most common genetic abnormality found in human embryos, and the leading genetic cause of implantation failure and miscarriage. Screening embryos for aneuploidy prior to their transfer during IVF should therefore provide a substantial benefit to many patients with infertility. Indeed, preimplantation genetic screening (PGS) is currently the subject of exciting advancements that may result in finally realizing the potential to improve the success of IVF and revolutionize the standard of care in reproductive medicine. The ideal of routine elective single embryo transfer may soon become a reality with the incorporation of valid comprehensive chromosome screening (CCS) technology. Furthermore, selective transfer of embryos predicted to possess a normal complement of 23 pairs of chromosomes not only represents an important clinical application, but also a unique opportunity to conduct more well-controlled research to develop additional prognostic markers of embryonic reproductive potential. While there are a large number of unique methodologies for CCS, this chapter will focus on results of research and development of two specific technologies.

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Author information

Authors and Affiliations

  1. Reproductive Medicine Associates of New Jersey, Basking Ridge, NJ, USA

    Nathan R. Treff

  2. Department of Obstetrics, Gynecology, and Reproductive Sciences, UMDNJ-Robert Wood Johnson Medical School, New Brunswick, NJ, USA

    Nathan R. Treff

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  1. Nathan R. Treff
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Correspondence to Nathan R. Treff .

Editor information

Editors and Affiliations

  1. University of Melbourne, Department of Zoology, Royal Parade, Melbourne, 3010, Victoria, Australia

    David K. Gardner

  2. Boston IVF, Second Ave. 130, Waltham, 02451, Massachusetts, USA

    Denny Sakkas

  3. Yale School of Medicine, Dept. of Ob/Gyn & Reproductive Sciences, Cedar St. 333, New Haven, 06510, Connecticut, USA

    Emre Seli

  4. Obstetrics & Gynaecology, University of Oxford, Nuffield Department of, Garsington Road, Oxford, OX4 2HW, Oxfordshire, United Kingdom

    Dagan Wells

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Treff, N.R. (2013). Quantitative SNP Array and Real-Time PCR-Based Human Preimplantation Embryo Aneuploidy Screening. In: Gardner, D., Sakkas, D., Seli, E., Wells, D. (eds) Human Gametes and Preimplantation Embryos. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6651-2_14

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  • DOI: https://doi.org/10.1007/978-1-4614-6651-2_14

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-4614-6650-5

  • Online ISBN: 978-1-4614-6651-2

  • eBook Packages: MedicineMedicine (R0)

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