Abstract
Interphase chromosome-specific multicolor banding (ICS-MCB) is a FISH-based technique for studying interphase chromosomes in their integrity at molecular resolution in single cells. To date, this is the only method providing for visualizing the whole chromosome and its specific regions together at any stage of the cell cycle. Offering such opportunity, ICS-MCB has repeatedly proven effective in molecular cytogenetic studies of interphase chromosomes (i.e., surveying genomic variations at the chromosomal level in different human tissues and characterizing interphase genome/chromosome organization and behavior). The intention of this chapter is to describe the basics of ICS-MCB procedure and its applications in different biomedical fields. The advantages of this technique allow speculations that it has to become a method of choice in somatic cell genetics as well as in cell and chromosome biology.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Claussen U (2005) Chromosomics. Cytogenet Genome Res 111:101–106
Cremer T, Cremer M (2010) Chromosome territories. Cold Spring Harb Perspect Biol 2:a003889
Iourov IY (2012) To see an interphase chromosome or: how a disease can be associated with specific nuclear genome organization. Biodiscovery 4:5. doi:10.7750/BioDiscovery.2012.4.5
Iourov IY, Liehr T, Vorsanova SG, Kolotii AD, Yurov YB (2006a) Visualization of interphase chromosomes in postmitotic cells of the human brain by multicolour banding (MCB). Chromosome Res 14:223–229
Iourov IY, Vorsanova SG, Pellestor F, Yurov YB (2006b) Brain tissue preparations for chromosomal PRINS labeling. Methods Mol Biol 334:123–132
Iourov IY, Vorsanova SG, Yurov YB (2006c) Chromosomal variation in mammalian neuronal cells: known facts and attractive hypotheses. Int Rev Cytol 249:143–191
Iourov IY, Liehr T, Vorsanova SG, Yurov YB (2007) Interphase chromosome-specific multicolor banding (ICS-MCB): a new tool for analysis of interphase chromosomes in their integrity. Biomol Eng 24:415–417
Iourov IY, Vorsanova SG, Yurov YB (2008a) Chromosomal mosaicism goes global. Mol Cytogenet 1:26
Iourov IY, Vorsanova SG, Yurov YB (2008b) Molecular cytogenetics and cytogenomics of brain diseases. Curr Genomics 9:452–465
Iourov IY, Vorsanova SG, Liehr T, Kolotii AD, Yurov YB (2009a) Increased chromosome instability dramatically disrupts neural genome integrity and mediates cerebellar degeneration in the ataxia-telangiectasia brain. Hum Mol Genet 18:2656–2669
Iourov IY, Vorsanova SG, Liehr T, Yurov YB (2009b) Aneuploidy in the normal, Alzheimer’s disease and ataxia-telangiectasia brain: differential expression and pathological meaning. Neurobiol Dis 34:212–220
Iourov IY, Vorsanova SG, Soloviev IV, Yurov YB (2009c) Interphase FISH: detection of intercellular genomic variations and somatic chromosomal mosaicism. In: Liehr T (ed) Fluorescence in situ hybridization (FISH): application guide. Springer, Berlin, pp 301–311
Iourov IY, Vorsanova SG, Yurov YB (2010) Somatic genome variations in health and disease. Curr Genomics 11:387–396
Lemke J, Claussen J, Michel S, Chudoba I, Mühlig P, Westermann M, Sperling K, Rubtsov N, Grummt UW, Ullmann P, Kromeyer-Hauschild K, Liehr T, Claussen U (2002) The DNA-based structure of human chromosome 5 in interphase. Am J Hum Genet 71:1051–1059
Liehr T (ed) (2009) Fluorescence in situ hybridization (FISH): application guide. Springer, Berlin
Liehr T, Heller A, Starke H, Rubtsov N, Trifonov V, Mrasek K, Weise A, Kuechler A, Claussen U (2002) Microdissection-based high resolution multicolor banding for all 24 human chromosomes. Int J Mol Med 9:335–339
Liehr T, Weise A, Hinreiner S, Mkrtchyan H, Mrasek K, Kosyakova N (2010) Characterization of chromosomal rearrangements using multicolor-banding (MCB/m-band). Methods Mol Biol 659:231–238
Manvelyan M, Hunstig F, Bhatt S, Mrasek K, Pellestor F, Weise A, Simonyan I, Aroutiounian R, Liehr T (2008a) Chromosome distribution in human sperm: a 3D multicolor banding-study. Mol Cytogenet 1:25
Manvelyan M, Hunstig F, Mrasek K, Bhatt S, Pellestor F, Weise A, Liehr T (2008b) Position of chromosomes 18, 19, 21 and 22 in 3D-preserved interphase nuclei of human and gorilla and white hand gibbon. Mol Cytogenet 1:9
Manvelyan M, Kempf P, Weise A, Mrasek K, Heller A, Lier A, Höffken K, Fricke HJ, Sayer HG, Liehr T, Mkrtchyan H (2009) Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics. Int J Mol Med 24:335–341
Vorsanova SG, Yurov YB, Iourov IY (2010a) Human interphase chromosomes: a review of available molecular cytogenetic technologies. Mol Cytogenet 3:1
Vorsanova SG, Yurov YB, Soloviev IV, Iourov IY (2010b) Molecular cytogenetic diagnosis and somatic genome variations. Curr Genomics 11:440–446
Weise A, Starke H, Heller A, Uwe C, Liehr T (2002) Evidence for interphase DNA decondensation transverse to the chromosome axis: a multicolor banding analysis. Int J Mol Med 9:359–361
Yurov YB, Iourov IY, Vorsanova SG, Liehr T, Kolotii AD, Kutsev SI, Pellestor F, Beresheva AK, Demidova IA, Kravets VS, Monakhov VV, Soloviev IV (2007) Aneuploidy and confined chromosomal mosaicism in the developing human brain. PLoS One 2:e558
Yurov YB, Iourov IY, Vorsanova SG, Demidova IA, Kravetz VS, Beresheva AK, Kolotii AD, Monakchov VV, Uranova NA, Vostrikov VM, Soloviev IV, Liehr T (2008) The schizophrenia brain exhibits low-level aneuploidy involving chromosome 1. Schizophr Res 98:139–147
Yurov YB, Vorsanova SG, Iourov IY (2010) Ontogenetic variation of the human genome. Curr Genomics 11:420–425
Acknowledgements
The authors are supported by DLR/BMBF (RUS 2011-2013) and RFBR grant 12-04-00215-а (Russian Federation, 2012–2014). The authors also acknowledge the NICHD Brain and Tissue Bank for Developmental Disorders at the University of Maryland, Baltimore, MD, USA, for providing the brain tissue samples studied by ICS-MCB (Fig. 9.2). Dr. IY Iourov is supported by the Grant of the President of the Russian Federation MD-4401.2013.7.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media, LLC
About this chapter
Cite this chapter
Iourov, I.Y., Liehr, T., Vorsanova, S.G., Yurov, Y.B. (2013). Interphase Chromosome-Specific Multicolor Banding. In: Yurov, Y., Vorsanova, S., Iourov, I. (eds) Human Interphase Chromosomes. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6558-4_9
Download citation
DOI: https://doi.org/10.1007/978-1-4614-6558-4_9
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4614-6557-7
Online ISBN: 978-1-4614-6558-4
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)