Abstract
The screening of cultivation conditions for the highest hydrogen production using mixed microflora was performed in serum bottles. The fermentation conditions were studied for temperature, inoculum size, and initial pH, and it shows the significant effect of these factors in enhancing the biohydrogen production. The optimum condition was found at 60 °C with initial pH of 7, and the percentage of inoculum added to POME medium was at 30:70 % (v/v). In the following experiment, batch thermophilic hydrogen fermentation was conducted in 0.5 L medium using the optimum conditions, which were run for 30 h. The highest yield of 4.85 mol H2/mol glucose was achieved when the fermentation was started with 4 g/L initial glucose concentration.
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Acknowledgments
The authors would like to acknowledge financial support from UKM-Sime Darby Foundation program on Sustainable Development–Zero Waste Technology for the Palm Oil Industry.
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Ibrahim, N., Jahim, J.M., S., L.S. (2013). Initial Study of Thermophilic Hydrogen Production from Raw Palm Oil Mill Effluent (POME) Using Mixed Microflora. In: Pogaku, R., Bono, A., Chu, C. (eds) Developments in Sustainable Chemical and Bioprocess Technology. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-6208-8_6
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DOI: https://doi.org/10.1007/978-1-4614-6208-8_6
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