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Model Systems for Studying Kisspeptin Signalling: Mice and Cells

Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 784)

Abstract

Kisspeptins are a family of overlapping neuropeptides, encoded by the Kiss1 gene, that are required for activation and maintenance of the mammalian reproductive axis. Kisspeptins act within the hypothalamus to stimulate release of gonadotrophic releasing hormone and activation of the pituitary-gonadal axis. Robust model systems are required to dissect the regulatory mechanisms that control Kiss1 neuronal activity and to examine the molecular consequences of kisspeptin signalling. While studies in normal animals have been important in this, transgenic mice with targeted mutations affecting the kisspeptin signalling pathway have played a significant role in extending our understanding of kisspeptin physiology. Knock-out mice recapitulate the reproductive defects associated with mutations in humans and provide an experimentally tractable model system to interrogate regulatory feedback mechanisms. In addition, transgenic mice with cell-specific expression of modulator proteins such as the CRE recombinase or fluorescent reporter proteins such as GFP allow more sophisticated analyses such as cell or gene ablation or electrophysiological profiling. At a less complex level, immortalized cell lines have been useful for studying the role of kisspeptin in cell migration and metastasis and examining the intracellular signalling events associated with kisspeptin signalling.

Keywords

Bacterial Artificial Chromosome GnRH Neuron Immortalize Cell Line Canonical Transient Receptor Potential Luteinizing Hormone Surge 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

We would like to thank Takeda Cambridge for their continued support, Ann Silver for proof reading the review and the animal house staff for excellent husbandry. This work was supported by a grant from the BBSRC (BB/F01936X/1) and the Ford Physiology Fund (WHC).

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© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Department of Physiology, Development and NeuroscienceUniversity of CambridgeCambridgeUK
  2. 2.Takeda CambridgeCambridgeUK
  3. 3.Department of Opthalmology, Cornea DivisionUniversity of CaliforniaLos AngelesUSA

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