Taurine 8 pp 395-403 | Cite as

Taurine May Not Alleviate Hyperglycemia-Mediated Endoplasmic Reticulum Stress in Human Adipocytes

  • Kyoung Soo KimEmail author
  • Hye-In Ji
  • Hyung-In Yang
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)


In obesity and diabetes, adipocytes show significant endoplasmic reticulum (ER) stress. Hyperglycemia-induced ER stress has not been studied in adipocyte differentiation and adipokine expression. Taurine has been known to protect the cells against ER stress. This study examined the effect of taurine on ER stress-induced adipocyte differentiation and adipokine expression to explain the therapeutic effect of taurine on diabetes and obesity. To do this, human preadipocytes were differentiated into adipocytes, in the presence or absence of taurine, under ER stress conditions. Changes in adipokine expression in adipocytes stimulated with IL-1β were investigated in the presence or absence of taurine. Human preadipocytes were treated with thapsigargin (10 nM) or high glucose concentrations (100 mM) as ER stress inducers during differentiation into adipocytes. Thapsigargin inhibited the differentiation of adipocytes in a dose-dependent manner, but the high glucose concentration treatment did not. Taurine 100 mM treatment did not block the inhibition of differentiation of preadipcytes into adipocytes. Furthermore, the high glucose concentration treatment inhibited the expression of adiponectin and increased the expression of leptin in human adipocytes. However, taurine treatment did not affect the expression of two adipokines. In conclusion, the therapeutic mechanism of taurine in diabetes and obesity does not appear to occur by alleviating hyperglycemia-mediated ER stress. To clarify the molecular mechanism by which taurine improves diabetic symptoms and obesity in animal models, the protective effect of taurine against hyperglycemia- or overnutrition-mediated ER stress should be further evaluated under various conditions or types of ER stress.


Endoplasmic Reticulum Stress Unfold Protein Response Adipocyte Differentiation High Glucose Concentration Human Adipocyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Endoplasmic reticulum




Type 2 diabetes mellitus


Tauroursodeoxycholic acid





This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2012-0002659).


  1. Alhusaini S, McGee K, Schisano B, Harte A, McTernan P, Kumar S, Tripathi G (2010) Lipopolysaccharide, high glucose and saturated fatty acids induce endoplasmic reticulum stress in cultured primary human adipocytes: salicylate alleviates this stress. Biochem Biophys Res Commun 397:472–478PubMedCrossRefGoogle Scholar
  2. Bachar E, Ariav Y, Ketzinel-Gilad M, Cerasi E, Kaiser N, Leibowitz G (2009) Glucose amplifies fatty acid-induced endoplasmic reticulum stress in pancreatic beta-cells via activation of mTORC1. PLoS One 4:e4954PubMedCrossRefGoogle Scholar
  3. Cnop M, Ladriere L, Igoillo-Esteve M, Moura RF, Cunha DA (2010) Causes and cures for endoplasmic reticulum stress in lipotoxic beta-cell dysfunction. Diabetes Obes Metab 12(Suppl 2):76–82PubMedCrossRefGoogle Scholar
  4. D’Hertog W, Maris M, Ferreira GB, Verdrengh E, Lage K, Hansen DA, Cardozo AK, Workman CT, Moreau Y, Eizirik DL, Waelkens E, Overbergh L, Mathieu C (2010) Novel insights into the global proteome responses of insulin-producing INS-1E cells to different degrees of endoplasmic reticulum stress. J Proteome Res 9:5142–5152PubMedCrossRefGoogle Scholar
  5. Daval M, Foufelle F, Ferre P (2006) Functions of AMP-activated protein kinase in adipose tissue. J Physiol 574:55–62PubMedCrossRefGoogle Scholar
  6. Ito T, Schaffer SW, Azuma J (2012) The potential usefulness of taurine on diabetes mellitus and its complications. Amino Acids 42:1529–1539PubMedCrossRefGoogle Scholar
  7. Kahn SE, Hull RL, Utzschneider KM (2006) Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature 444:840–846PubMedCrossRefGoogle Scholar
  8. Kamiya T, Obara A, Hara H, Inagaki N, Adachi T (2011) ER stress inducer, thapsigargin, decreases extracellular-superoxide dismutase through MEK/ERK signalling cascades in COS7 cells. Free Radic Res 45:692–698PubMedCrossRefGoogle Scholar
  9. Kim HM, Do CH, Lee DH (2010) Taurine reduces ER stress in C. elegans. J Biomed Sci 17(Suppl 1):S26PubMedCrossRefGoogle Scholar
  10. Kim SJ, Gupta RC, Lee HW (2007) Taurine-diabetes interaction: from involvement to protection. Curr Diabetes Rev 3:165–175PubMedCrossRefGoogle Scholar
  11. Lourenco R, Camilo ME (2002) Taurine: a conditionally essential amino acid in humans? An overview in health and disease. Nutr Hosp 17:262–270PubMedGoogle Scholar
  12. Ozcan U, Cao Q, Yilmaz E, Lee AH, Iwakoshi NN, Ozdelen E, Tuncman G, Gorgun C, Glimcher LH, Hotamisligil GS (2004) Endoplasmic reticulum stress links obesity, insulin action, and type 2 diabetes. Science 306:457–461PubMedCrossRefGoogle Scholar
  13. Pan C, Prentice H, Price AL, Wu JY (2011) Beneficial effect of taurine on hypoxia- and glutamate-induced endoplasmic reticulum stress pathways in primary neuronal culture. Amino Acids 43(2):845–855, Epub ahead of printPubMedCrossRefGoogle Scholar
  14. Ramirez-Zacarias JL, Castro-Munozledo F, Kuri-Harcuch W (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97:493–497PubMedCrossRefGoogle Scholar
  15. Shimada T, Hiramatsu N, Okamura M, Hayakawa K, Kasai A, Yao J, Kitamura M (2007) Unexpected blockade of adipocyte differentiation by K-7174: implication for endoplasmic reticulum stress. Biochem Biophys Res Commun 363:355–360PubMedCrossRefGoogle Scholar
  16. van der Kallen CJ, van Greevenbroek MM, Stehouwer CD, Schalkwijk CG (2009) Endoplasmic reticulum stress-induced apoptosis in the development of diabetes: is there a role for adipose tissue and liver? Apoptosis 14:1424–1434PubMedCrossRefGoogle Scholar
  17. Xu L, Spinas GA, Niessen M (2010) ER stress in adipocytes inhibits insulin signaling, represses lipolysis, and alters the secretion of adipokines without inhibiting glucose transport. Horm Metab Res 42:643–651PubMedCrossRefGoogle Scholar
  18. Zha BS, Zhou H (2012) ER stress and lipid metabolism in adipocytes. Biochem Res Int 2012:312943PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.East-West Bone & Joint Disease Research InstituteKyung Hee University Hospital at GangdongSeoulKorea

Personalised recommendations