Abstract
Taurine (2-aminoethanesulfonic acid), a sulfur-containing β-amino acid, is found in all animal cells at millimolar concentrations and has been reported to show various health promoting activities including antidiabetic properties. The beneficial effects of taurine in diabetes mellitus have been known. However, the exact mechanism of hypoglycemic action of taurine is not properly defined. In this study, we investigated antidiabetic effect of taurine in the cell culture system using rat skeletal muscle cells. In cultured rat skeletal L6 myotubes, we studied the effect of taurine (0–100 μM) on glucose uptake to plasma membrane from the aspects of AMP-activated protein kinase (AMPK) signaling. Taurine stimulated glucose uptake in a dose-dependent manner by activating AMPK signaling. From these results, it may suggest that taurine show antidiabetic effect by stimulating insulin-independent glucose uptake in rat skeletal muscle.
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- AMPK:
-
AMP-activated protein kinase
- PI3K:
-
Phosphatidylinositol-3 kinase
- GLUT4:
-
Glucose transporter 4
References
Aerts L, Van Assche FA (2002) Taurine and taurine-deficiency in the perinatal period. J Perinat Med 30:281–286. doi:10.1515/JPM.2002.040
Brons C, Spohr C, Storgaard H, Dyerberg J, Vaag A (2004) Effect of taurine treatment on insulin secretion and action on serum lipid levels in overweighted men with genetic predisposition for type II diabetes mellitus. Eur J Clin Nutr 58:1239–1247. doi:10.1038/sj.ejcn.1601955
Brosnan JT, Brosnan ME (2006) The sulfur-containing amino acids: an overview. J Nutr 136: 1636S–1640S
Carneiro EM, Latorraca MQ, Araujo E, Beltrá M, Oliveras MJ, Navarro M, Berná G, Bedoya FJ, Velloso LA, Soria B, Martin F (2009) Taurine supplementation modulates glucose homeostasis and islet function. J Nutr Biochem 20:503–511. doi:10.1016/j.jnutbio.2008.05.00810.1016/j.jnutbio. 2008.05.008
Chang KJ (2000) Effect of taurine and β-alanine on morphological changes of pancreas in streptozotocin-induced rats. Adv Exp Med Biol 483:571–577. doi:10.1007/0-306-46838-7_61
Cherif H, Reusens B, Ahn MT, Hoet JJ, Remacle C (1998) Effects of taurine on the insulin secretion of rat fetal islets from dams fed a low-protein diet. J Endocrinol 159:341–348. doi:10.1677/joe.0.1590341
Colivicchi MA, Raimondi L, Bianchi L, Tipton KF, Pirisino R, Della Corte L (2004) Taurine prevents streptozotocin impairment of hormone-stimulated glucose uptake in rat adipocytes. Eur J Pharmacol 495:209–215. doi:10.1016/j.ejphar.2004.05.004
Das J, Ghosh J, Manna PM, Sil PC (2008) Taurine provides antioxidant defense against NaF-induced cytotoxicity in murine hepatocytes. Pathophysiology 15:181–190. doi:10.1016/j.pathophys.2008.06.002
Das J, Ghosh J, Manna P, Sinha M, Sil PC (2009) Taurine protects rat testes against NaAsO2-induced oxidative stress and apoptosis via mitochondrial dependent and independent pathways. Toxicol Lett 187:201–210. doi:10.1016/j.toxlet.2009.03.001
De la Puerta C, Arrieta FJ, Balsa JA, Botella-Carretero JI, Zamarron I, Vazquez C (2010) Taurine and glucose metabolism: a review. Nutr Hosp 25:910–919. doi:10.3305/nh.2010.25.6.4815
Doi M, Yamaoka I, Fukunaga T, Nakayama M (2003) Isoleucine, a potent plasma glucose-lowering amino acid, stimulates glucose uptake in C2C12myotubes. Biochem Biophys Res Commun 312:1111–1117. doi:10.1016/j.bbrc.2003.11.039
El Idrissi A, Trenkner E (2004) Taurine as a modulator of excitatory and inhibitory neurotransmission. Neurochem Res 1:189–197. doi:10.1023/B:NERE.0000010448.17740.6e
Franconi F, Di Leo MA, Bennardini F, Ghirlanda G (2004) Is taurine beneficial in reducing risk factors for diabetes mellitus? Neurochem Res 29:143–150
Herman MA, Kahn BB (2006) Glucose transport and sensing in the maintenance of glucose homeostasis and metabolic harmony. J Clin Invest 116:1767–1775. doi:10.1172/JCI29027
Jessen N, Goodyear LJ (2005) Contraction signaling to glucose transport in skeletal muscle. J Appl Physiol 99:330–337. doi:10.1152/japplphysiol.00175.2005
Kulakowski EC, Maturo J (1984) Hypoglycemic properties of taurine: not mediated by enhanced insulin release. Biochem Pharmacol 33:2835–2838. doi:10.1016/0006-2952(84)90204-1
Lampson WG, Kramer JH, Schaffer SW (1983) Potentiation of the actions of insulin by taurine. Can J Physiol Pharmacol 61:457–463. doi:10.1139/y83-070
Magnoni LJ, Vraskou Y, Palstra AP, Planas JV (2012) AMP-activated protein kinase plays an important evolutionary conserved role in the regulation of glucose metabolism in fish skeletal muscle cells. PLoS One 7:e31219. doi:10.1371/journal.pone.0031219
Manna P, Das J, Ghosh J, Sil PC (2010) Contribution of type 1 diabetes to rat liver dysfunction and cellular damage via activation of NOS, PARP, IκBα/NF-κB, MAPKs, and mitochondria-dependent pathways: prophylactic role of arjunolic acid. Free Radic Biol Med 48:1465–1484. doi:10.1016/j.freeradbiomed.2010.02.025
Maturo J, Kulakowski EC (1988) Taurine binding to the purified insulin receptor. Biochem Pharmacol 37:3755–3760. doi:10.1016/0006-2952(88)90411-X
Moller DE (2001) New drug targets for type 2 diabetes and the metabolic syndrome. Nature 414:821–827. doi:10.1038/414821a
Pasantes-Morales H, Wright CE, Gaull GE (1985) Taurine protection of lymphoblastoid cells from iron-ascorbate-induced damage. Biochem Pharmacol 34:2205–2207. doi:10.1016/0006-2952(85)90419-8
Racasan S, Braam B, van der Giezen DM, Goldschmeding R, Boer P, Koomans HA (2004) Perinatal L-arginine and antioxidant supplements reduce adult blood pressure in spontaneously hypertensive rats. Hypertension 44:83–88. doi:10.1161/01.HYP.0000133251.40322.20
Ribeiro RA, Bonfleur ML, Amaral AG, Vanzela EC, Rocco SA, Boschero AC, Carneiro EM (2009) Taurine supplementation enhances nutrient-induced insulin secretion in pancreatic mice islets. Diabetes Metab Res Rev 25:370–379. doi:10.1002/dmrr.959
Saltiel AR, Kahn CR (2001) Insulin signaling and the regulation of glucose and lipid metabolism. Nature 414:799–806. doi:10.1038/414799a
Schaffer SW, Azuma J, Mozaffari M (2009) Role of antioxidant activity of taurine in diabetes. Can J Physiol Pharmacol 87:91–99. doi:10.1139/Y08-110
Sinha M, Manna P, Sil PC (2007) Taurine, a conditionally essential amino acid, ameliorates arsenic-induced cytotoxicity in murine hepatocytes. Toxicol In Vitro 21:1419–1428. doi:10.1016/j.tiv.2007.05.010
Solon CS, Franci D, Ignacio-Souza LM, Romanatto T, Roman EA, Arruda AP, Morari J, Torsoni AS, Carneiro EM, Velloso LA (2011) Taurine enhances the anorexigenic effects of insulin in the hypothalamus of rats. Amino Acids. doi:10.1007/s00726-011-1045-5
Thurston JH, Hauhart RE, Dirgo JA (1980) Taurine: a role in osmotic regulation of mammalian brain and possible clinical significance. Life Sci 26:1561–1568. doi:10.1016/0024-3205(80)90358-6
Towler MC, Hardie DG (2007) AMP-activated protein kinase in metabolic control and insulin signaling. Circ Res 100:328–341. doi:10.1161/01.RES.0000256090.42690.05
Zou MH, Kirkpatrick SS, Davis BJ, Nelson JS, Wiles WG IV, Schlattner U, Neumann D, Brownlee M, Freeman MB, Goldman MH (2004) Activation of the AMP-activated protein kinase by the anti-diabetic drug metformin in vivo. Role of mitochondrial reactive nitrogen species. J Biol Chem 279:43940–43951. doi:10.1074/jbc.M404421200
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Cheong, S.H., Chang, K.J. (2013). Antidiabetic Effect of Taurine in Cultured Rat Skeletal L6 Myotubes. In: El Idrissi, A., L'Amoreaux, W. (eds) Taurine 8. Advances in Experimental Medicine and Biology, vol 775. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6130-2_26
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DOI: https://doi.org/10.1007/978-1-4614-6130-2_26
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