Inhibitory Effects of Taurine on STZ-Induced Apoptosis of Pancreatic Islet Cells
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The present research aims to investigate the inhibition effect of taurine on the apoptosis of pancreatic islet cells induced by Streptozotocin (STZ). One hundred male Wistar rats weighing 180–200 g were randomly divided into two groups, rats in the experimental were intraperitoneally injected with 2% STZ (50 mg/kg bw, dissolved in 0.1 mmol/L pH 4.2 citrate buffer), rats in the control group were injected with the same volume of citrate buffer. Rats with the fasting blood glucose level higher than 16.7 mmol/L were selected and randomly divided into four groups: Rats in M group were STZ-induced diabetes rats, rats in T1, T2, and T3 groups were intragastrically administered with taurine (dissolved in 0.5% sodium carboxymethyl cellulose as thickening agent) once a day for 4 weeks with the contents of 0.6 g/kg, 1.2 g/kg, and 2.4 g/kg bw, respectively, while rats in group C and M were given the same amount of thickening agent as T2 group. Four weeks later, pancreatic tissues were fixed and processed for paraffin section. The results showed that STZ induced a significant increase in apoptotic rate of pancreatic islet cells, up-regulated the expression of bax and Fas and down-regulated the expression of Bcl-2, which were significantly blocked by taurine (P < 0.05). The results indicated that taurine could significantly restrain apoptosis of pancreatic islet cells induced by STZ.
KeywordsIslet Cell Integral Optical Density Pancreatic Islet Cell Taurine Supplementation Pancreatic Gland
Financial support from the National Research Foundation of China (Project no. 30972154) and Doctoral initiaitng project of liaoning province (Project no. 20111083) is acknowledged.
- Garchon HJ, Luan JJ, Eloy L, Bédossa P, Bach JF (1994) Genetic analysis of immune dysfunction in non-obese diabetic (NOD) mice: mapping of a susceptibility locus close to the Bcl-2 gene correlates with increased resistance of NOD T cells to apoptosis induction. Eur J Immunol 24(2):380–384PubMedCrossRefGoogle Scholar
- Liu D, Cardozo AK, Darville MI, Eizirik DL (2002) Double-stranded RNA cooperates with interferon-gamma and IL-1 beta to induce both chemokine expression and nuclear factor-kappa B-dependent apoptosis in pancreatic beta-cells: potential mechanisms for viral-induced insulitis and beta-cell death in type 1 diabetes mellitus. Endocrinology 143:1225–1234PubMedCrossRefGoogle Scholar
- Szkudelski T (2001) The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 50:536–546Google Scholar
- Taranukhin AG, Taranukhina EY, Saransaari P, Pelto-Huikko M, Podkletnova M, Oja S (2012) Taurine protects cerebellar neurons of the external granular layer against ethanol-induced apoptosis in 7-day-old mice. Amino Acids. doi:10.1007/s00726-012-1254-6Google Scholar