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Taurine 8 pp 269-283 | Cite as

Influence of Taurine Haloamines (TauCl and TauBr) on the Development of Pseudomonas aeruginosa Biofilm: A Preliminary Study

  • Janusz MarcinkiewiczEmail author
  • Magdalena Strus
  • Maria Walczewska
  • Agnieszka Machul
  • Diana Mikołajczyk
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)

Abstract

Biofilms are consortia of microorganisms (sessile cells) that form on various surfaces including mucosal membranes or teeth. Bacterial biofilms cause many human infections such as chronic sinusitis, acne vulgaris, periodontal diseases, and chronic wounds. These infections are persistent as they show increased resistance to antibiotics and host defense system. Taurine chloramine (TauCl) and taurine bromamine (TauBr) are the physiological products of activated neutrophils, resulting from the reaction between taurine with hypochlorous acid (HOCl) and hypobromous acid (HOBr), respectively. It has been shown in vitro that taurine haloamines exert antimicrobial properties against various pathogenic bacteria. Moreover, clinical studies have shown that both haloamines are effective in the local treatment of skin and mucose infections, including biofilm-related infections. Nevertheless, it has been not tested yet whether they can kill bacteria hidden in biofilm or disrupt biofilm structure. In this study we have investigated the capacity of TauCl and TauBr to inhibit in vitro the formation of P. aeruginosa biofilm. We have also tested their ability to destroy the mature biofilm. Our results suggest that TauBr is able to inhibit in vitro the formation of P. aeruginosa biofilm but cannot destroy the mature biofilm and effectively killed hidden bacteria. In further studies, the combined effect of TauBr and DNase, one of suggested biofilm inhibitors, was tested. Together, we conclude that TauBr is a better than TauCl candidate for local therapy of biofilm-related infections. However, a combined therapy, an application of TauBr together with other anti-biofilm agents (e.g., DNase), seems to be more promising.

Keywords

Extracellular Polymeric Substance Planktonic Form Sessile Cell Taurine Chloramine Hypobromous Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

Tau

Taurine

TauCl

Taurine chloramine

TauBr

Taurine bromamine

P. aeruginosa

Pseudomonas aeruginosa

CFU

Colony forming units

Notes

Acknowledgments

We want to thank Prof. Waldemar Gottardi and Prof. Marcus Nagl from the Division of Hygiene and Medical Microbiology, Innsbruck Medical University, Austria, for giving us N-chlorotaurine sodium salt. This paper was supported by Jagiellonian University Medical College grant No K/ZDS/002964, grant No K/ZDS/002861 and grant N N 401 547 040.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Janusz Marcinkiewicz
    • 1
    Email author
  • Magdalena Strus
    • 2
  • Maria Walczewska
    • 1
  • Agnieszka Machul
    • 2
  • Diana Mikołajczyk
    • 2
  1. 1.Department of ImmunologyJagiellonian University Medical CollegeCracowPoland
  2. 2.Department of MicrobiologyJagiellonian University Medical CollegeCracowPoland

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