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Taurine 8 pp 259-267 | Cite as

Taurine Chloramine Administered In Vivo Increases NRF2-Regulated Antioxidant Enzyme Expression in Murine Peritoneal Macrophages

  • In Soon Kang
  • Chaekyun KimEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)

Abstract

Taurine chloramine (TauCl) is produced from taurine by the ­myeloperoxidase-halide system in activated neutrophils via a stoichiometric reaction between taurine and HOCl. TauCl has been shown to provide cytoprotection against inflammatory tissue injury by inhibiting the overproduction of inflammatory mediators and also by increasing the expression of antioxidant enzymes that are regulated by nuclear factor E2-related factor 2 in murine macrophages. In this study, primary murine macrophages were prepared after either by injection of 3% thioglycolate into mouse peritoneal cavity or by differentiation of the isolated bone marrow cells. TauCl increased HO-1, Prx-1, and Trx-1 expression in murine primary ­macrophages. Also, when TauCl was injected in combination with 3% thioglycolate, HO-1 expression in the peritoneal macrophages was increased. Our results suggest that TauCl plays a protective role against cytotoxicity of oxidative stress in macrophages by increasing the expression of antioxidant enzymes in vivo.

Keywords

Peritoneal Macrophage Antioxidant Response Element Murine Peritoneal Macrophage Free Heme Antioxidant Enzyme Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ARE

Antioxidant response element

BMDM

Bone marrow-derived macrophages

GPx

Glutathione peroxidase

HO

Heme oxygenase

Keap

Kelch-like ECH-associated protein

LPS

Lipopolysaccharide

M-CSF

Macrophage colony stimulating factor

MMP

Metalloproteinase

MPO

Myeloperoxidase

Nrf2

Nuclear factor E2-related factor

Prx

Peroxiredoxin

TauCl

Taurine chloramine

TNF-α

Tumor necrosis factor-α

Trx

Thioredoxin

Notes

Acknowledgments

We thank Dr. Young-Nam Cha for discussions throughout the study and critical comments on the manuscript, and Mi Ran Cho for the technical support. This work was supported by the NRF of Korea grant funded by the Korea government MEST (2012R1A1A3007097) and Inha University research grant.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Laboratory for Leukocyte Signaling Research, Department of Pharmacology and Toxicology, Inha Research Institute for Medical ScienceInha University School of MedicineIncheonKorea

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