Taurine Chloramine Administered In Vivo Increases NRF2-Regulated Antioxidant Enzyme Expression in Murine Peritoneal Macrophages
Taurine chloramine (TauCl) is produced from taurine by the myeloperoxidase-halide system in activated neutrophils via a stoichiometric reaction between taurine and HOCl. TauCl has been shown to provide cytoprotection against inflammatory tissue injury by inhibiting the overproduction of inflammatory mediators and also by increasing the expression of antioxidant enzymes that are regulated by nuclear factor E2-related factor 2 in murine macrophages. In this study, primary murine macrophages were prepared after either by injection of 3% thioglycolate into mouse peritoneal cavity or by differentiation of the isolated bone marrow cells. TauCl increased HO-1, Prx-1, and Trx-1 expression in murine primary macrophages. Also, when TauCl was injected in combination with 3% thioglycolate, HO-1 expression in the peritoneal macrophages was increased. Our results suggest that TauCl plays a protective role against cytotoxicity of oxidative stress in macrophages by increasing the expression of antioxidant enzymes in vivo.
KeywordsPeritoneal Macrophage Antioxidant Response Element Murine Peritoneal Macrophage Free Heme Antioxidant Enzyme Gene
Antioxidant response element
Bone marrow-derived macrophages
Kelch-like ECH-associated protein
Macrophage colony stimulating factor
Nuclear factor E2-related factor
Tumor necrosis factor-α
We thank Dr. Young-Nam Cha for discussions throughout the study and critical comments on the manuscript, and Mi Ran Cho for the technical support. This work was supported by the NRF of Korea grant funded by the Korea government MEST (2012R1A1A3007097) and Inha University research grant.
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