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Taurine 8 pp 247-257 | Cite as

Effect of Taurine Chloramine on Differentiation of Human Preadipocytes into Adipocytes

  • Kyoung Soo KimEmail author
  • Hyun-Mi Choi
  • Hye-In Ji
  • Chaekyun Kim
  • Jung Yeon Kim
  • Ran Song
  • So-Mi Kim
  • Yeon-Ah Lee
  • Sang-Hoon Lee
  • Hyung-In Yang
  • Myung Chul Yoo
  • Seung-Jae HongEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)

Abstract

We investigated whether taurine chloramine (TauCl), which is ­endogenously produced by immune cells such as macrophages that infiltrate adipose tissue, affects the differentiation of preadipocytes into adipocytes or modulates the expression of adipokines in adipocytes. To study the physiological effects of TauCl on human adipocyte differentiation and adipokine expression, preadipocytes were cultured under differentiation conditions for 14 days in the presence or the absence of TauCl. Differentiated adipocytes were also treated with TauCl in the presence or the absence of IL-1β (1 ng/ml) for 7 days. The culture supernatants were analyzed for adipokines such as adiponectin, leptin, IL-6, and IL-8. At concentrations of 400–600 μM, TauCl significantly inhibited the differentiation of human preadipocytes into adipocytes in a dose-dependent manner. It did not induce the dedifferentiation of adipocytes or inhibit fat accumulation in adipocytes. Expression of major transcription factors of adipogenesis and adipocyte marker genes was decreased after treatment with TauCl, in agreement with its inhibition of ­differentiation. These results suggest that TauCl may inhibit the differentiation of ­preadipocytes into adipocytes. Thus, TauCl or more stable derivatives of TauCl could potentially be a safe drug therapy for obesity-related diseases.

Keywords

Adipose Tissue Adipocyte Differentiation Major Transcription Factor Intracellular Lipid Accumulation Taurine Chloramine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

MSCs

Mesenchymal stem cells

C/EBP

CCAAT/enhancer-binding protein

PPARg

Peroxisome proliferator-activated receptor γ

FABP

Fatty acid-binding protein

LPL

Lipoprotein lipase

GLUT4

Glucose transporter 4

TauCl

Taurine chloramine

HOCl

Hypochlorous acid

Notes

Acknowledgments

This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0024089 and 2011-0009061).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Kyoung Soo Kim
    • 1
    Email author
  • Hyun-Mi Choi
    • 1
  • Hye-In Ji
    • 1
  • Chaekyun Kim
    • 2
  • Jung Yeon Kim
    • 3
  • Ran Song
    • 4
  • So-Mi Kim
    • 4
  • Yeon-Ah Lee
    • 4
  • Sang-Hoon Lee
    • 4
  • Hyung-In Yang
    • 4
  • Myung Chul Yoo
    • 1
  • Seung-Jae Hong
    • 4
    Email author
  1. 1.East-West Bone & Joint Disease Research InstituteKyung Hee University Hospital at GangdongSeoulKorea
  2. 2.Laboratory for Leukocyte Signaling Research, Department of Pharmacology and BK21 ProgramInha University School of MedicineIncheonKorea
  3. 3.Department of PathologyInje University Sanggye Paik HospitalSeoulKorea
  4. 4.Division of RheumatologyKyung Hee University Medical SchoolSeoulKorea

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