Taurine 8 pp 247-257 | Cite as

Effect of Taurine Chloramine on Differentiation of Human Preadipocytes into Adipocytes

  • Kyoung Soo KimEmail author
  • Hyun-Mi Choi
  • Hye-In Ji
  • Chaekyun Kim
  • Jung Yeon Kim
  • Ran Song
  • So-Mi Kim
  • Yeon-Ah Lee
  • Sang-Hoon Lee
  • Hyung-In Yang
  • Myung Chul Yoo
  • Seung-Jae HongEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)


We investigated whether taurine chloramine (TauCl), which is ­endogenously produced by immune cells such as macrophages that infiltrate adipose tissue, affects the differentiation of preadipocytes into adipocytes or modulates the expression of adipokines in adipocytes. To study the physiological effects of TauCl on human adipocyte differentiation and adipokine expression, preadipocytes were cultured under differentiation conditions for 14 days in the presence or the absence of TauCl. Differentiated adipocytes were also treated with TauCl in the presence or the absence of IL-1β (1 ng/ml) for 7 days. The culture supernatants were analyzed for adipokines such as adiponectin, leptin, IL-6, and IL-8. At concentrations of 400–600 μM, TauCl significantly inhibited the differentiation of human preadipocytes into adipocytes in a dose-dependent manner. It did not induce the dedifferentiation of adipocytes or inhibit fat accumulation in adipocytes. Expression of major transcription factors of adipogenesis and adipocyte marker genes was decreased after treatment with TauCl, in agreement with its inhibition of ­differentiation. These results suggest that TauCl may inhibit the differentiation of ­preadipocytes into adipocytes. Thus, TauCl or more stable derivatives of TauCl could potentially be a safe drug therapy for obesity-related diseases.


Adipose Tissue Adipocyte Differentiation Major Transcription Factor Intracellular Lipid Accumulation Taurine Chloramine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



Mesenchymal stem cells


CCAAT/enhancer-binding protein


Peroxisome proliferator-activated receptor γ


Fatty acid-binding protein


Lipoprotein lipase


Glucose transporter 4


Taurine chloramine


Hypochlorous acid



This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0024089 and 2011-0009061).


  1. Apovian CM, Bigornia S, Mott M, Meyers MR, Ulloor J, Gagua M, McDonnell M, Hess D, Joseph L, Gokce N (2008) Adipose macrophage infiltration is associated with insulin resistance and vascular endothelial dysfunction in obese subjects. Arterioscler Thromb Vasc Biol 28: 1654–1659PubMedCrossRefGoogle Scholar
  2. Ariel A, Serhan CN (2007) Resolvins and protectins in the termination program of acute inflammation. Trends Immunol 28:176–183PubMedCrossRefGoogle Scholar
  3. Bjorntorp P, Berchtold P, Tibblin G (1971) Insulin secretion in relation to adipose tissue in men. Diabetes 20:65–70PubMedGoogle Scholar
  4. Cho EJ, Rahman MA, Kim SW, Baek YM, Hwang HJ, Oh JY, Hwang HS, Lee SH, Yun JW (2008) Chitosan oligosaccharides inhibit adipogenesis in 3T3-L1 adipocytes. J Microbiol Biotechnol 18:80–87PubMedGoogle Scholar
  5. Choi HM, Lee YA, Lee SH, Hong SJ, Hahm DH, Choi SY, Yang HI, Yoo MC, Kim KS (2009) Adiponectin may contribute to synovitis and joint destruction in rheumatoid arthritis by stimulating vascular endothelial growth factor, matrix metalloproteinase-1, and matrix metalloproteinase-13 expression in fibroblast-like synoviocytes more than proinflammatory mediators. Arthritis Res Ther 11:R161PubMedCrossRefGoogle Scholar
  6. Compher C, Badellino KO (2008) Obesity and inflammation: lessons from bariatric surgery. JPEN J Parenter Enteral Nutr 32:645–647PubMedCrossRefGoogle Scholar
  7. Coppack SW (2001) Pro-inflammatory cytokines and adipose tissue. Proc Nutr Soc 60:349–356PubMedCrossRefGoogle Scholar
  8. Gades MD, Stern JS (2005) Chitosan supplementation and fat absorption in men and women. J Am Diet Assoc 105:72–77PubMedCrossRefGoogle Scholar
  9. Gesta S, Tseng YH, Kahn CR (2007) Developmental origin of fat: tracking obesity to its source. Cell 131:242–256PubMedCrossRefGoogle Scholar
  10. Gregoire FM, Smas CM, Sul HS (1998) Understanding adipocyte differentiation. Physiol Rev 78:783–809PubMedGoogle Scholar
  11. Halberg N, Wernstedt-Asterholm I, Scherer PE (2008) The adipocyte as an endocrine cell. Endocrinol Metab Clin North Am 37:753–768, x-xiPubMedCrossRefGoogle Scholar
  12. Ho SC, Tai ES, Eng PH, Tan CE, Fok AC (2001) In the absence of dietary surveillance, chitosan does not reduce plasma lipids or obesity in hypercholesterolaemic obese Asian subjects. Singapore Med J 42:6–10PubMedGoogle Scholar
  13. Ito T, Schaffer SW, Azuma J (2011) The potential usefulness of taurine on diabetes mellitus and its complications. Amino Acids 42(5):1529–1539PubMedCrossRefGoogle Scholar
  14. Itoh M, Suganami T, Hachiya R, Ogawa Y (2011) Adipose tissue remodeling as homeostatic inflammation. Int J Inflam 2011:720926PubMedGoogle Scholar
  15. Jessen BA, Stevens GJ (2002) Expression profiling during adipocyte differentiation of 3T3-L1 fibroblasts. Gene 299:95–100PubMedCrossRefGoogle Scholar
  16. Kim KS, Park EK, Ju SM, Jung HS, Bang JS, Kim C, Lee YA, Hong SJ, Lee SH, Yang HI, Yoo MC (2007) Taurine chloramine differentially inhibits matrix metalloproteinase 1 and 13 synthesis in interleukin-1beta stimulated fibroblast-like synoviocytes. Arthritis Res Ther 9:R80PubMedCrossRefGoogle Scholar
  17. Kirkland JL, Hollenberg CH, Gillon WS (1990) Age, anatomic site, and the replication and differentiation of adipocyte precursors. Am J Physiol 258:C206–C210PubMedGoogle Scholar
  18. Klover PJ, Clementi AH, Mooney RA (2005) Interleukin-6 depletion selectively improves hepatic insulin action in obesity. Endocrinology 146:3417–3427PubMedCrossRefGoogle Scholar
  19. MacDougald OA, Mandrup S (2002) Adipogenesis: forces that tip the scales. Trends Endocrinol Metab 13:5–11PubMedCrossRefGoogle Scholar
  20. Ramirez-Zacarias JL, Castro-Munozledo F, Kuri-Harcuch W (1992) Quantitation of adipose conversion and triglycerides by staining intracytoplasmic lipids with Oil red O. Histochemistry 97:493–497PubMedCrossRefGoogle Scholar
  21. Ronti T, Lupattelli G, Mannarino E (2006) The endocrine function of adipose tissue: an update. Clin Endocrinol (Oxf) 64:355–365Google Scholar
  22. Rosen ED, Hsu CH, Wang X, Sakai S, Freeman MW, Gonzalez FJ, Spiegelman BM (2002) C/EBPalpha induces adipogenesis through PPARgamma: a unified pathway. Genes Dev 16:22–26PubMedCrossRefGoogle Scholar
  23. Rosen ED, MacDougald OA (2006) Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol 7:885–896PubMedCrossRefGoogle Scholar
  24. Rotter V, Nagaev I, Smith U (2003) Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overexpressed in human fat cells from insulin-resistant subjects. J Biol Chem 278:45777–45784PubMedCrossRefGoogle Scholar
  25. Roufosse CA, Direkze NC, Otto WR, Wright NA (2004) Circulating mesenchymal stem cells. Int J Biochem Cell Biol 36:585–597PubMedCrossRefGoogle Scholar
  26. Schuller-Levis GB, Park E (2004) Taurine and its chloramine: modulators of immunity. Neurochem Res 29:117–126PubMedCrossRefGoogle Scholar
  27. Shehzad A, Ha T, Subhan F, Lee YS (2011) New mechanisms and the anti-inflammatory role of curcumin in obesity and obesity-related metabolic diseases. Eur J Nutr 50:151–161PubMedCrossRefGoogle Scholar
  28. Sowers MR, Karvonen-Gutierrez CA (2010) The evolving role of obesity in knee osteoarthritis. Curr Opin Rheumatol 22:533–537PubMedCrossRefGoogle Scholar
  29. Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Bergmann O, Blomqvist L, Hoffstedt J, Naslund E, Britton T, Concha H, Hassan M, Ryden M, Frisen J, Arner P (2008) Dynamics of fat cell turnover in humans. Nature 453:783–787PubMedCrossRefGoogle Scholar
  30. Szymanski K, Winiarska K (2008) [Taurine and its potential therapeutic application]. Postepy Hig Med Dosw (Online) 62:75–86Google Scholar
  31. Thomas EL, Grisham MB, Melton DF, Jefferson MM (1985) Evidence for a role of taurine in the in vitro oxidative toxicity of neutrophils toward erythrocytes. J Biol Chem 260:3321–3329PubMedGoogle Scholar
  32. Tsuboyama-Kasaoka N, Shozawa C, Sano K, Kamei Y, Kasaoka S, Hosokawa Y, Ezaki O (2006) Taurine (2-aminoethanesulfonic acid) deficiency creates a vicious circle promoting obesity. Endocrinology 147:3276–3284PubMedCrossRefGoogle Scholar
  33. Weyer C, Foley JE, Bogardus C, Tataranni PA, Pratley RE (2000) Enlarged subcutaneous abdominal adipocyte size, but not obesity itself, predicts type II diabetes independent of insulin resistance. Diabetologia 43:1498–1506PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Kyoung Soo Kim
    • 1
    Email author
  • Hyun-Mi Choi
    • 1
  • Hye-In Ji
    • 1
  • Chaekyun Kim
    • 2
  • Jung Yeon Kim
    • 3
  • Ran Song
    • 4
  • So-Mi Kim
    • 4
  • Yeon-Ah Lee
    • 4
  • Sang-Hoon Lee
    • 4
  • Hyung-In Yang
    • 4
  • Myung Chul Yoo
    • 1
  • Seung-Jae Hong
    • 4
    Email author
  1. 1.East-West Bone & Joint Disease Research InstituteKyung Hee University Hospital at GangdongSeoulKorea
  2. 2.Laboratory for Leukocyte Signaling Research, Department of Pharmacology and BK21 ProgramInha University School of MedicineIncheonKorea
  3. 3.Department of PathologyInje University Sanggye Paik HospitalSeoulKorea
  4. 4.Division of RheumatologyKyung Hee University Medical SchoolSeoulKorea

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