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Taurine 8 pp 237-246 | Cite as

Protection by Taurine Against INOS-Dependent DNA Damage in Heavily Exercised Skeletal Muscle by Inhibition of the NF-κB Signaling Pathway

  • Hiromichi Sugiura
  • Shinya Okita
  • Toshihiro Kato
  • Toru Naka
  • Shosuke Kawanishi
  • Shiho Ohnishi
  • Yoshiharu Oshida
  • Ning MaEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)

Abstract

Taurine protects against tissue damage in a variety of models involving inflammation, especially the muscle. We set up a heavy exercise bout protocol for rats consisting of climbing ran on a treadmill to examine the effect of an intraabdominal dose of taurine (300 mg/kg/day) administered 1 h before heavy exercise for ten consecutive days. Each group ran on the treadmill at 20 m/min, 25% grade, for 20 min or until exhaustion within 20 min once each 10 days. Exhaustion was the point when an animal was unable to right itself when placed on its side. The muscle damage was associated with an increased accumulation of 8-nitroguanine and 8-OHdG in the nuclei of skeletal muscle cells. The immunoreactivities for NF-κB and iNOS were also increased in the exercise group. Taurine ameliorated heavy exercise-induced muscle DNA damage to a significant extent since it reduced the accumulation of 8-nitroguanine and 8-OHdG, possibly by down-regulating the expression of iNOS through a modulatory action on NF-κB signaling pathway. This study demonstrates for the first time that taurine can protect against intense exercise-induced nitrosative inflammation and ensuing DNA damage in the skeletal muscle of rats by preventing iNOS expression and the nitrosative stress generated by heavy exercise.

Keywords

Soleus Muscle Skeletal Muscle Cell Reactive Nitrogen Species Nitrosative Stress Heavy Exercise 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

ROS

Reactive oxygen species

O2

Superoxide anion

NF-κB

Nuclear factor kappa-B

iNOS

Inducible NO synthase

8-OHdG

8-Hydroxydeoxyguanosine

Notes

Acknowledgments

This work was supported by Grants-in-Aid for Scientific Research from the Ministry of Education, Science, Sports, and Culture of Japan.

References

  1. Aruoma OI, Halliwell B, Hoey BM, Butler J (1988) The antioxidant action of taurine, hypotaurine and their metabolic precursors. Biochem J 256:251–255PubMedGoogle Scholar
  2. Barua M, Liu Y, Quinn MR (2001) Taurine chloramine inhibits inducible nitric oxide synthase and TNF-alpha gene expression in activated alveolar macrophages: decreased NF-κB activation and IκB kinase activity. J Immunol 167:2275–2281PubMedGoogle Scholar
  3. Brooks GA, White TP (1978) Determination of metabolic and heart rate responses of rats to treadmill exercise. J Appl Physiol 45:1009–1015PubMedGoogle Scholar
  4. Gurujeyalashmi G, Wang Y, Giri SN (2000) Taurine and niacin block lung and fibrosis by ­down-regulating bleomycin-induced activation of transcription nuclear factor-κB in mice. J Pharmacol Exp Ther 293:82–90Google Scholar
  5. Hansen SH, Andersen ML, Birkedal H, Cornett C, Wibrand F (2006) The important role of taurine in oxidative metabolism. Adv Exp Med Biol 583:129–135PubMedCrossRefGoogle Scholar
  6. Ji LL, Gomez-Cabrera MC, Steinhafel N, Vina J (2004) Acute exercise activates nuclear factor (NF)-κB signaling pathway in rat skeletal muscle. FASEB J 18:1499–1506PubMedCrossRefGoogle Scholar
  7. Loeb LA, Preston BD (1986) Mutagenesis by a apurinic/apyrimidinic sites. Annu Rev Genet 20:201–230PubMedCrossRefGoogle Scholar
  8. Ma N, Adachi Y, Hiraku Y, Horiki N, Horike S, Imoto I, Pinlaor S, Murata M, Semba R, Kawanishi S (2004) Accumulation of 8-nitroguanine in human gastric epithelium induced byHelicobacter pyloriinfection. Biochem Biophys Res Commun 319:506–510PubMedCrossRefGoogle Scholar
  9. Moyes CD (2003) Controlling muscle mitochondrial content. J Exp Biol 206:4385–4391PubMedCrossRefGoogle Scholar
  10. Nikolaidis MG, Mougios V (2004) Effects of exercise on the fatty-acid composition of blood and tissue lipids. Sports Med 34:1051–1076PubMedCrossRefGoogle Scholar
  11. Nikolaidis MG, Petridou A, Mougios V (2006) Comparison of the phospholipid and triacylglycerol fatty acid profile of rat serum, skeletal muscle and heart. Physiol Res 55:259–265PubMedGoogle Scholar
  12. Pinlaor S, Hiraku Y, Ma N, Yongvanit P, Semba R, Oikawa S, Murata M, Sripa B, Sithithaworn P, Kawanishi S (2004) Mechanism of NO-mediated oxidative and nitrative DNA damage in hamsters infected with Opisthorchis viverrini: a model of inflammation-mediated carcinogenesis. Nitric Oxide 11:175–183PubMedCrossRefGoogle Scholar
  13. Prutz WA (1996) Hypochlorous acid interactions with thiols, nucleotides, DNA, and other biological substrates. Arch Biochem Biophys 332:110–120PubMedCrossRefGoogle Scholar
  14. Sen CK, Khanna S, Resznick AZ, Roy S, Packer L (1997) Glutathione regulation of tumor necrosis factor-α-induced NF-κB activation in skeletal muscle-derived L6 cells. Biochem Biophys Res Commun 237:645–649PubMedCrossRefGoogle Scholar
  15. Shi X, Flynn DC, Porter DW, Leonard SS, Vallyathan V, Castranova V (1997) Efficacy of taurine based compounds as hydroxyl radical scavengers in silica induced peroxidation. Ann Clin Lab Sci 27:365–374PubMedGoogle Scholar
  16. Silva LA, Silveira PC, Ronsani MM, Souza PS, Scheffer D, Vieira LC, Benetti M, De Souza CT, Pinho RA (2011) Taurine supplementation decreases oxidative stress in skeletal muscle after eccentric exercise. Cell Biochem Funct 29:43–49PubMedCrossRefGoogle Scholar
  17. Yermilov V, Rubio J, Becchi M, Friesen MD, Pignatelli B, Ohshima H (1995) Formation of 8-nitroguanine by the reaction of guanine with peroxynitritein vitro. Carcinogenesis 16: 2045–2050PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Hiromichi Sugiura
    • 1
    • 2
  • Shinya Okita
    • 1
  • Toshihiro Kato
    • 1
  • Toru Naka
    • 1
  • Shosuke Kawanishi
    • 1
  • Shiho Ohnishi
    • 1
  • Yoshiharu Oshida
    • 2
  • Ning Ma
    • 1
    Email author
  1. 1.Faculty of Health Science and Pharmaceutical SciencesSuzuka University of Medical ScienceMieJapan
  2. 2.Department of Sports Medicine, Graduate School of MedicineNagoya UniversityNagoyaJapan

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