Thiotaurine Prevents Apoptosis of Human Neutrophils: A Putative Role in Inflammation
- 2.1k Downloads
Thiotaurine, a metabolic product of cystine, contains a sulfane sulfur atom that can be released as H2S, a gaseous molecule with a regulatory activity on inflammatory responses. The influence of thiotaurine on human leukocyte spontaneous apoptosis has been evaluated by measuring caspase-3 activity in human neutrophils. Addition of 100 μM thiotaurine induced a 55% inhibition of caspase-3 activity similar to that exerted by 100 μM H2S. Interestingly, in the presence of 1 mM GSH, an increase of the inhibition of apoptosis by thiotaurine has been observed. These results indicate that the bioactivity of thiotaurine can be modulated by GSH, which promotes the reductive breakdown of the thiosulfonate generating H2S and hypotaurine. As thiotaurine is able to incorporate reversibly reduced sulfur, it is suggested that the biosynthesis of this thiosulfonate could be a means to transport and store H2S.
KeywordsHuman Neutrophil Neutrophil Apoptosis Spontaneous Apoptosis Sodium Hydrosulfide Preincubation Step
- Acharya M, Lau-Cam CA (2012) Comparative evaluation of the effects of hypotaurine, taurine and thiotaurine on alterations of the cellular redox status and activities of antioxidant and glutathione-related enzymes by acetaminophen in the rat. Amino Acids 42:1512Google Scholar
- De Marco C, Coletta M, Cavallini D (1961) Spontaneous transulfuration of sulfinates by organic persulfides. Arch Biochem Biophys 93:179–180Google Scholar
- Nicholson DW, Ali A, Thornberry NA, Vaillancourt JP, Ding CK, Gallant M, Gareau Y, Griffin PR, Labelle M, Lazebnik YA, Munday NA, Raju SM, Smulson ME, Yamin T-T, Yu VL, Miller DK (1995) Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature 376:37–43PubMedCrossRefGoogle Scholar