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Taurine 8 pp 167-175 | Cite as

Taurine Exerts Robust Protection Against Hypoxia and Oxygen/Glucose Deprivation in Human Neuroblastoma Cell Culture

  • Po-Chih Chen
  • Chunliu Pan
  • Payam M. Gharibani
  • Howard PrenticeEmail author
  • Jang-Yen WuEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)

Abstract

Stroke is one of the leading causes of mortality and disability worldwide. There is no effective treatment for stroke despite extensive research. Taurine is a free amino acid which is present at high concentrations in a range of organs including the brain, heart, and retina in mammalian systems. It had been shown that taurine can significantly increase cell survival under stroke conditions using both in vivo and in vitro models. Recently, we have found that several agents including granulocyte colony-stimulating factor (G-CSF), a stem cell enhancer and facilitator;S-methyl-N-diethylthiolcarbamate sulfoxide (DETC-MeSO), an NMDA receptor partial antagonist; sulindac, a potent antioxidant; and taurine, a neuroprotectant and calcium regulator, are effective in protecting against stroke-induced neuronal injury when used alone or in combination in both animal and tissue/cell culture models. In this chapter, we demonstrate that taurine can protect human neuroblastoma cells measured by ATP assay under conditions of hypoxia or oxygen/glucose deprivation (OGD). In addition, we found that taurine exerts its protective function by suppressing the OGD-induced upregulation of endoplasmic reticulum (ER) stress markers and proapoptotic proteins. A model depicting the mode of action of taurine in protecting neuroblastoma cells under OGD conditions is presented.

Keywords

Endoplasmic Reticulum Stress Oxygen Glucose Deprivation Hypoxia Group Taurine Treatment Oxygen Glucose Deprivation Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Biomedical ScienceCharles E. Schmidt College of Medicine, Florida Atlantic UniversityBoca RatonUSA
  2. 2.Department of Neurology and Sleep CenterShung Ho Hospital, Taipei Medical UniversityTaipeiTaiwan

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