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Taurine 8 pp 155-166 | Cite as

Protective Effect of Taurine on Down-Regulated Expression of Thyroid Hormone Receptor Genes in Brains of Mice Exposed to Arsenic

  • Yachen Wang
  • Fengyuan PiaoEmail author
  • Yachen Li
  • Xianghu Wang
  • Huai Guang
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 775)

Abstract

This study aimed at evaluating protective effect of taurine on the down-regulated expressions of thyroid hormone receptor (TR) genes in brains of mice exposed to arsenic (As). The SPF mice were randomly divided into As exposure group, protective group, and control group. The As exposure group was administered with 4 ppm As2O3 through drinking water for 60 days. The protective group was treated with both 4 ppm As2O3 and 150 mg/kg taurine. The control group was given with drinking water alone. The gene expressions of TR in the mouse brains of the three groups were analyzed by real-time PCR. Their protein expressions were examined by Western blot and immunohistochemistry. Our results showed that the gene expression of TRβ, a very important regulator of Camk4 transcription, was down-regulated in cerebral and cerebellar tissues of mice exposed to As. The expression of TRβ1 protein in the cerebral or cerebellar tissue significantly decreased in the group exposed to As compared to the control group. However, the expressions of TRβ gene and TRβ1 protein were significantly rescued in the group coadministered with taurine as antioxidant. These results indicated that taurine may have the protective effect on the down-regulated expressions of TR in brains of mice exposed to As.

Keywords

Thyroid Hormone Receptor Sodium Arsenite Monoclone Antibody Severe Pathological Change Mouse Cerebrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

TR

Thyroid hormone receptor

As

Arsenic

LTP

Long-term potentiation

LTD

Long-term depression

Creb

cAMP response element-binding protein

TRE

Thyroid hormone-response element

Notes

Acknowledgements

This work was supported by National Nature Science Foundation of China [No. 30571584].

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Yachen Wang
    • 1
  • Fengyuan Piao
    • 1
    Email author
  • Yachen Li
    • 1
  • Xianghu Wang
    • 1
  • Huai Guang
    • 1
  1. 1.Department of Occupational and Environmental HealthDalian Medical UniversityDalianP.R. China

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