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Taurine 8 pp 59-66 | Cite as

The Effects of Bisphosphonates on Taurine Transport in Retinal Capillary Endothelial Cells Under High Glucose Conditions

  • Na-Young Lee
  • Young-Sook KangEmail author
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 776)

Abstract

Diabetic retinopathy (DR) is a major cause of blindness in diabetic patients. Elevated glucose and vascular endothelial growth factor (VEGF) in retina can trigger many of the retinal vascular changes caused by diabetes and DR. Recently, bisphosphonates, antiosteoporosis drugs, have been reported to have anti-angiogenic effect by decreasing VEGF. Taurine has several biological processes such as osmoregulation and antioxidation in retina. Therefore, the purpose of this study is to clarify the regulation of taurine transport activity by high glucose concentration and the effect of inhibitors for VEGF function, bisphosphonates, on taurine transport under high glucose condition using TR-iBRB cell lines as an in vitro model of inner blood-retinal barrier (iBRB). As a result, by exposing TR-iBRB cells to high glucose for 48 h, [3H]taurine uptake was decreased continuously. [3H]Taurine uptake was increased significantly by pretreatment of alendronate and pamidronate compared with the values for high glucose. Increased [3H]taurine uptake by pretreatment of alendronate and pamidronate was significantly reduced by mevalonate pathway intermediates, geranylgeraniol (GGOH). In conclusion, taurine transport through the iBRB under high glucose condition can be regulated by bisphosphonates via mevalonate pathway. Therefore, we suggest that bisphosphonates could have the beneficial effects on DR by regulation of taurine contents in retina.

Keywords

Vascular Endothelial Growth Factor Diabetic Retinopathy Osteogenesis Imperfecta Retinal Pigment Epithelial Cell Mevalonate Pathway 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

iBRB

Inner blood-retinal barrier

DR

Diabetic retinopathy

VEGF

Vascular endothelial growth factor

GGOH

Geranylgeraniol

TAUT

Taurine transporter

Notes

Acknowledgements

This work was supported by the Research Grant of Sookmyung Women’s University.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.College of Pharmacy and Research Center for Cell Fate ControlSookmyung Women’s UniversitySeoulSouth Korea

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