Taurine 8 pp 335-345 | Cite as

Effect of Dietary Taurine and Arginine Supplementation on Bone Mineral Density in Growing Female Rats

Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 776)

Abstract

The purpose of this study was to determine the effect of arginine or ­taurine alone and taurine plus arginine on bone mineral density (BMD) and markers of bone formation and bone resorption in growing female rats. Forty female SD rats (75 ± 5 g) were randomly divided into four groups (control, taurine, arginine, taurine + arginine group) and treatment lasted for 9 weeks. All rats were fed on a diet and deionized water. BMD and bone mineral content (BMC) were measured using PIXImus (GE Lunar Co, Wisconsin, USA) in spine and femur. The serum and urine concentrations of calcium and phosphorus were determined. Bone formation was measured by serum osteocalcin and alkaline phosphatase concentrations, and the bone resorption rate was measured by deoxypyridinoline cross-links. Femur BMD was significantly increased in the group with taurine supplementation and femur BMC/weight was significantly increased in the group with arginine + taurine supplementation. Rats fed an arginine or taurine supplemental diet increased femur BMD or femur BMC, but a taurine + arginine-supplemented diet does not have a better effect than arginine or taurine alone in the spine BMD. The femur BMC, expressed per body weight, was higher in arginine + taurine group than in the taurine or arginine group. The results of this study suggest that taurine + arginine supplementation may be beneficial on femur BMC in growing female rats. Additional work is needed to clarify the interactive effects between the taurine and arginine to determine whether dietary intakes of arginine and taurine affect bone quality in growing rats.

Keywords

Phosphorus Magnesium Osteoporosis Cage Creatinine 

Abbreviations

ALP

Alkaline phosphatase

DPD

Deoxypyridinoline

Arg

Arginine

Tau

Taurine

Arg + Tau

Arginine + taurine

DPD/Cr

Creatinine excretion

AI

Adequate intake

BMD

Bone mineral density

BMC

Bone mineral content

FER

Food efficiency ratio

SBMD

Spine bone mineral density

SBMC

Spine bone mineral content

FBMD

Femur bone mineral density

FBMC

Femur bone mineral content

Notes

Acknowledgements

This research was supported by the Bisa Research Grant of Keimyung University in 2010.

References

  1. Azuma J, Sawamura A, Awata N (1992) Usefulness of taurine in chronic congestive heart failure and its prospective application. Jpn Circ J 56:95–99PubMedCrossRefGoogle Scholar
  2. Chen W, Nishimura N, Oda H, Yohogoshi H (2003) Effect of taurine on cholesterol degradation and bile acid pool in rats fed a high-cholesterol diet. Taurine 5: beginning the 21st century. Adv Exp Med Biol 526:261–267PubMedCrossRefGoogle Scholar
  3. Choi MJ (2007a) Effects of arginine supplementation on bone mineral density in growing female rats. Korean J Nutr 40:235–241Google Scholar
  4. Choi MJ (2007b) Effects of arginine supplementation on bone markers and hormones in growing female rats. Korean J Nutr 42:1–9Google Scholar
  5. Choi MJ (2009) Effects of taurine supplementation on bone mineral density in overiectomized rats fed calcium deficient diet. Nutr Res Pract 3:108–113PubMedCrossRefGoogle Scholar
  6. Choi MJ, DiMarco NM (2009) The effects of dietary taurine supplementation on bone mineral density in ovariectomized rat. Adv Exp Med Biol 643:341–349PubMedCrossRefGoogle Scholar
  7. Choi MJ, Jo HJ (2003) Effects of soy and isoflavones on bone metabolism in growing female rats. Korean J Nutr 36:549–558Google Scholar
  8. Choi MJ, Seo JN (2006) The effect of dietary taurine supplementation on plasma and liver lipid concentrations in rats. J East Asian Soc Dietary Life 16:121–127Google Scholar
  9. Chung YH (2001) The effect of dietary taurine on skeletal metabolism in ovariectomized rats. Korean J Hum Ecol 4:84–93Google Scholar
  10. Frost HM (2000) Utah paradigm of skeletal physiology: an overview of its insights for bone, cartilage and collagenous tissue organs. J Bone Miner Metab 18:305–316PubMedCrossRefGoogle Scholar
  11. Fulgoni VL III, Huth PJ, DiRienzo DB, Miller GD (2004) Determination of the optimal number of dairy servings to ensure a low prevalence of inadequate calcium intakes in Americans. J Am Coll Nutr 23:651–659PubMedGoogle Scholar
  12. Garcia RAG, Stipanuk MH (1992) The splanchnic organs, liver and kidney have unique roles in the metabolism of sulfur amino acids and their metabolites in rats. J Nutr 122:1693–1701PubMedGoogle Scholar
  13. Heaney RP (2007) Does daily calcium supplementation reduce the risk of clinical fractures in elderly women? Nat Rev Rheumatol 3:18–19Google Scholar
  14. Heaney RP, Abrams S, Dawson-Hughes B, Looker A, Marcus R, Matkovic V, Weaver C (2000) Peak bone mass. Osteoporos Int 11:985–1009PubMedCrossRefGoogle Scholar
  15. Heys SD, Gardner E (1999) Nutrients and the surgical patient: current and potential therapeutic applications to clinical practice. J R Coll Surg Edinb 44:283–293PubMedGoogle Scholar
  16. Ho-Pham LT, Nguyen ND, Nguyen TV (2009) Effect of vegetarian diets on bone mineral density: a Bayesian meta-analysis. Am J Clin Nutr 90:943–950Google Scholar
  17. Inderjeeth CA, Chan K, Kwan K, Lie M (2012) Time to onset of efficacy in fracture reduction with current anti-osteoporosis treatments. J Bone Miner Metab 30:493–503. doi:10.1007/s00774-012-0349-1PubMedCrossRefGoogle Scholar
  18. Kim KL, Kim WY (1983) The effect of soy protein and casein on serum lipid, amino acid. Korean J Nutr 17:309–310Google Scholar
  19. Luiking YC, Deutz NEP (2007) Biomarkers of arginine and lysine excess. J Nutr 137(6):1662S–1668SPubMedGoogle Scholar
  20. Micha R, Wallace SK, Mozaffarian D (2010) Red and processed meat consumption and risk of incident coronary heart disease, stroke, and diabetes mellitus: a systematic review and meta-analysis. Circulation 121:2271–2283PubMedCrossRefGoogle Scholar
  21. Mundy GR (2006) Nutritional modulators of bone remodeling during aging. Am J Clin Nutr 83:427S–430SPubMedGoogle Scholar
  22. Nakaya Y, Minami A, Harada N, Sakamoto S, Niwa Y, Ohnaka M (2000) Taurine improves insulin sensitivity in the Otsuka long-evans tokushima fatty rat, a model of spontaneous type 2 diabetes. Am J Clin Nutr 71:154–158Google Scholar
  23. Newsholme EA, Leech AR (1983) Biochemistry for the medical sciences. Wiley, New YorkGoogle Scholar
  24. Nieves JW (2005) Osteoporosis: the role of micronutrients. Am J Clin Nutr 81:1232S–1239SPubMedGoogle Scholar
  25. Park SY, Kim H, Kim SJ (2001) Stimulation of ERK2 by taurine with enhanced alkaline phosphatase activity and collagen synthesis in osteoblast-like UMR-106 cells. Biochem Pharmacol 62:1107–1111PubMedCrossRefGoogle Scholar
  26. Schuller-Levis GB, Park E (2003) Taurine: new implications for an old amino acid. FEMS Microbiol Lett 226:195–202PubMedCrossRefGoogle Scholar
  27. Sluijs I, Beulens JW, Vander A DL, Spijkerman AM, Van der Schouw YT (2010) Dietary intake of total, animal, and vegetable protein and risk of type 2 diabetes in the European prospective investigation into cancer and nutrition (EPIC)-NL study. Diabetes Care 33:43–48PubMedCrossRefGoogle Scholar
  28. Sugiyama K, Ohishi A, Ohnuma Y, Muramarsu K (1989) Comparison between the plasma cholesterol-lowering effects of glycine and taurine in rats fed on high cholesterol diets. Agric Biol Chem 53(6):1647–1652CrossRefGoogle Scholar
  29. Takahahsi K, Azuma M, Baba A, Schaffer S, Azuma J (1998) Taurine improves angiotensin II induced hypertrophy of cultured neonatal rat heart cells. Adv Exp Med Biol 442:129–135PubMedGoogle Scholar
  30. Thacher TD, Fischer PR, Strand MA, Pettifor JM (2006) Nutritional rickets around the world: causes and future directions. Ann Trop Paediatr 26:1–16PubMedCrossRefGoogle Scholar
  31. Visek WJ (1986) Arginine needs, physiological state and United diets. A reevaluation. J Nutr 116:36–46PubMedGoogle Scholar
  32. Wells BJ, Mainous AG III, Everett CJ (2005) Association between dietary arginine and C-reactive protein. Nutrition 21:125–130PubMedCrossRefGoogle Scholar
  33. Wheatley BP (2005) An evaluation of sex and body weight determination from the proximal femur using DXA technology and its potential for forensic anthropology. Forensic Sci Int 29(147):141–145CrossRefGoogle Scholar
  34. Windmueller HG, Spaeth AE (1981) Source and fate of circulating citrulline. Am J Physiol 241:E473–E480PubMedGoogle Scholar
  35. Wong WW, Lewis RD, Steinberg FM, Murray MJ, Cramer MA, Amato P, Young RL, Barnes S, Ellis KJ, Shypailo RJ, Fraley JK, Konzelmann KL, Fischer JG, Smith EO (2009) Soy isoflavone supplementation and bone mineral density in menopausal women: a 2-y multicenter clinical trial. Am J Clin Nutr 90:1433–1439PubMedCrossRefGoogle Scholar
  36. Yu CH, Lee YS, Lee JS (1998) Some factors effect in bone density of Korean college women. Korean J Nutr 31:36–45Google Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Food and NutritionKeimyung UniversityDaeguSouth Korea
  2. 2.Department of Food and NutritionInha UniversityIncheonSouth Korea

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