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Differential Regulation of TauT by Calcitriol and Retinoic Acid via VDR/RXR in LLC-PK1 and MCF-7 Cells

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Taurine 8

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 776))

Abstract

The interaction between taurine and the absorption of fat-soluble ­vitamins, such as vitamin A and D, has been an interesting topic in the field of ­nutrition science, because taurine-conjugated bile acid optimizes fat and fat-soluble vitamin absorption. However, whether the hormone calcitriol (1,25-dihydroxyvitamin D3) and retinoic acid regulate the expression of the TauT gene is unknown. In this study, we test the hypothesis that the TauT gene is regulated by vitamin D3 (VD3) and retinoic acid (RA) via activation of the vitamin D receptor (VDR) and retinoic acid receptor (RXR). Taurine uptake, Western blotting, gene reporter assay, and immunohistochemical analysis of TauT, VDR, and RXR were used in VD3- and/or RA-treated LLC-PK1 and MCF-7 cells. We demonstrated that VD3 alone had little effect on TauT expression in both LLC-PK1 and MCF-7 cells. Expression of TauT was significantly increased by RA, which was synergized by the addition of VD3 after RXR activation in LLC-PK1 cells. In contrast, expression of TauT was significantly decreased by the combination of VD3 and RA in MCF-7 cells. Regulation of TauT by VD3/RA appears to occur at the transcriptional level, as determined by a reporter gene assay of the TauT promoter. Immunohistochemical study showed that VDR and RXR were activated by VD3 and RA, respectively, in both LLC-PK1 and MCF-7 cells. The activated VDR and RXR also colocated in nuclei of both cells, suggesting that a VDR/RXR complex is involved in the transcriptional regulation of TauT. Our results show that expression of TauT is differentially regulated by VD3 and RA via formation of VDR and RXR complexes in the nuclei in a cell type-dependent manner.

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Abbreviations

TauT:

Taurine transporter gene

VDR:

Vitamin D receptor

RXR:

Retinoic acid receptor

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Acknowledgements

The authors wish to thank Andrea Patters for insightful comments and suggestions. This work was supported by the Le Bonheur Chair of Excellence in Pediatrics.

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Authors and Affiliations

  1. Division of Nephrology, Department of Pediatrics, University of Tennessee Health Science Center, Le Bonheur Children’s Hospital, Children’s Foundation Research Tower, Rm 476R, 50 North Dunlap, 38103, Memphis, TN, USA

    Russell W. Chesney M.D. & Xiaobin Han M.D., Ph.D.

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  1. Russell W. Chesney M.D.
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  2. Xiaobin Han M.D., Ph.D.
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Correspondence to Russell W. Chesney M.D. .

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Editors and Affiliations

  1. City University of New York, Center for Developmental Neuroscience, College of Staten Island, 2800 Victory Blvd, Staten Island, 10314, New York, USA

    Abdeslem El Idrissi

  2. City University of New York, Department of Biology, College of Staten Island, Victory Blvd 2800, Staten Island, 10314, New York, USA

    William J. L'Amoreaux

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Chesney, R.W., Han, X. (2013). Differential Regulation of TauT by Calcitriol and Retinoic Acid via VDR/RXR in LLC-PK1 and MCF-7 Cells. In: El Idrissi, A., L'Amoreaux, W. (eds) Taurine 8. Advances in Experimental Medicine and Biology, vol 776. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-6093-0_27

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