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Taurine 8 pp 291-305 | Cite as

Differential Regulation of TauT by Calcitriol and Retinoic Acid via VDR/RXR in LLC-PK1 and MCF-7 Cells

  • Russell W. ChesneyEmail author
  • Xiaobin Han
Conference paper
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 776)

Abstract

The interaction between taurine and the absorption of fat-soluble ­vitamins, such as vitamin A and D, has been an interesting topic in the field of ­nutrition science, because taurine-conjugated bile acid optimizes fat and fat-soluble vitamin absorption. However, whether the hormone calcitriol (1,25-dihydroxyvitamin D3) and retinoic acid regulate the expression of the TauT gene is unknown. In this study, we test the hypothesis that the TauT gene is regulated by vitamin D3 (VD3) and retinoic acid (RA) via activation of the vitamin D receptor (VDR) and retinoic acid receptor (RXR). Taurine uptake, Western blotting, gene reporter assay, and immunohistochemical analysis of TauT, VDR, and RXR were used in VD3- and/or RA-treated LLC-PK1 and MCF-7 cells. We demonstrated that VD3 alone had little effect on TauT expression in both LLC-PK1 and MCF-7 cells. Expression of TauT was significantly increased by RA, which was synergized by the addition of VD3 after RXR activation in LLC-PK1 cells. In contrast, expression of TauT was significantly decreased by the combination of VD3 and RA in MCF-7 cells. Regulation of TauT by VD3/RA appears to occur at the transcriptional level, as determined by a reporter gene assay of the TauT promoter. Immunohistochemical study showed that VDR and RXR were activated by VD3 and RA, respectively, in both LLC-PK1 and MCF-7 cells. The activated VDR and RXR also colocated in nuclei of both cells, suggesting that a VDR/RXR complex is involved in the transcriptional regulation of TauT. Our results show that expression of TauT is differentially regulated by VD3 and RA via formation of VDR and RXR complexes in the nuclei in a cell type-dependent manner.

Keywords

Retinoic Acid Polar Bear Taurine Transport Taurine Content Taurine Uptake 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

TauT

Taurine transporter gene

VDR

Vitamin D receptor

RXR

Retinoic acid receptor

Notes

Acknowledgements

The authors wish to thank Andrea Patters for insightful comments and suggestions. This work was supported by the Le Bonheur Chair of Excellence in Pediatrics.

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Division of Nephrology, Department of PediatricsUniversity of Tennessee Health Science Center, Le Bonheur Children’s Hospital, Children’s Foundation Research TowerMemphisUSA

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