The Influence of Experimentally Reduced Vaginal Flora in Oestrus Females on the Mating Behaviour of Male Domestic Dogs (Canis familiaris)

  • Michał Dzięcioł
  • Wojciech Niżański
  • Roland Kozdrowski
  • Małgorzata Ochota
  • Ewa Stańczyk


This study assessed the effect of reduced vaginal flora during oestrus on the mating behaviour of four male dogs. The experiment included a total of 32 bitches in oestrus that were allocated to three groups. Group A included untreated bitches (n = 15), group B, bitches treated by an intravaginal infusion of an antibiotic (n = 15) and group C, bitches treated by an intramuscular injection of antibiotic (n = 15). Bitches in oestrus (i.e. with progesterone concentrations of 10–15 ng/mL) were presented to the male dogs. Our results demonstrate that antibiotic-treated bitches with reduced vaginal bacterial flora (females in groups B and C) were less attractive to males than untreated females. There was no significant difference between the two methods of antibiotic administration. These results indicate that vaginal flora produces semiochemical substances (pheromones) during oestrus that induce sexual arousal in male dogs.


Sexual Arousal Clavulanic Acid Bacterial Flora Vaginal Discharge Sexual Attractiveness 
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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Michał Dzięcioł
    • 1
  • Wojciech Niżański
    • 1
  • Roland Kozdrowski
    • 1
  • Małgorzata Ochota
    • 1
  • Ewa Stańczyk
    • 1
  1. 1.Department of Reproduction and Clinic of Farm AnimalsWrocław University of Environmental and Life SciencesWrocławPoland

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