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The Recessus Olfactorius: A Cryptic Olfactory Organ of Anuran Amphibians

  • Christine Nowack
  • Sabrina Jordan
  • Carolin Wittmer
Chapter

Abstract

The recessus olfactorius is a presumably olfactory structure found on the rostral floor of the main nasal cavity of many anuran amphibians. Nevertheless, it has rarely been investigated or even recognized in the past. The aim of the present study was to broaden the knowledge regarding this enigmatic sensory organ. Therefore, we examined the nasal epithelia of the cane toad Bufo marinus and the fire-bellied toad Bombina orientalis, using histology, scanning electron microscopy and lectin histochemistry.

Histologically, the recessus olfactorius largely resembles a typical pseudostratified olfactory epithelium. However, we found significant differences when comparing the recessus olfactorius with the main olfactory organ and the vomeronasal organ. The surface differentiations of the recessus olfactorius can be assigned to four different cell types: sensory cells covered with either cilia located on protruding knobs or with long microvilli, and supporting cells bearing either short microvilli or cilia without any surface elevation. Additionally, the lectin binding patterns indicate the presence of a unique mucous constitution of the recessus olfactorius.

Taken together our results strongly support the idea, that the recessus olfactorius has a discrete olfactory function. Furthermore, a possible homology between the recessus olfactorius and the middle chamber epithelium of clawed frogs like Xenopus laevis is discussed.

Keywords

Supporting Cell Olfactory Epithelium Sensory Epithelium Cane Toad Olfactory Receptor Neuron 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgements

The authors would like to sincerely thank Harald Rühling, Julia Schulze, and Andreas Arendt for their very helpful technical support. Parts of this research were supported by the Zentrale Forschungsförderung (ZFF) of the University of Kassel (grant number 1415, P/249).

References

  1. Altner H (1962) Untersuchungen über Leistungen und Bau der Nase des südafrikanischen Krallenfrosches Xenopus laevis (Daudin, 1803). Z vergl Physiol 45:272–306CrossRefGoogle Scholar
  2. Brooks SA, Lymboura M, Schumacher U, Leathem AJ (1996) Histochemistry to detect Helix pomatia lectin binding in breast cancer: methodology makes a difference. J Histochem Cytochem 44:519–524PubMedCrossRefGoogle Scholar
  3. Du Toit CA (1933) Some aspects of the cranial morphology of Rana grayi Smith. Proc Zool Soc Lond 103:715–734CrossRefGoogle Scholar
  4. Etzler ME, Kabat EA (1970) Purification and characterization of a lectin (plant hemagglutinin) with blood group A specificity from Dolichos biflorus. Biochemistry 9:869–877PubMedCrossRefGoogle Scholar
  5. Hansen A, Reiss JO, Gentry CL, Burd GD (1998) Ultrastructure of the olfactory organ in the clawed frog, Xenopus laevis, during larval development and metamorphosis. J Comp Neurol 398:273–288PubMedCrossRefGoogle Scholar
  6. Helling H (1938) Das Geruchsorgan der Anuren, vergleichend-morphologisch betrachtet. Z Anat 108:587–643CrossRefGoogle Scholar
  7. Jungblut LD, Pozzi AG, Paz DA (2011) Larval development and metamorphosis of the olfactory and vomeronasal organs in the toad Rhinella (Bufo) arenarum (Hensel, 1867). Acta Zool 92:305–315CrossRefGoogle Scholar
  8. Meyer DL, Jadhao AG, Bhargava S, Kicliter E (1996) Bulbar representation of the ‘water-nose’ during Xenopus ontogeny. Neurosci L 220:109–122CrossRefGoogle Scholar
  9. Moulton D, Beidler L (1967) Structure and function in the peripheral olfactory system. Physiol Rev 47:1–52PubMedGoogle Scholar
  10. Nowack C (2011) Functional anatomy of the lateral nasal gland in anuran amphibians and its relation to the vomeronasal organ. J Herpetol 45:511–515CrossRefGoogle Scholar
  11. Nowack C, Wöhrmann-Repenning A (2010) The nasolacrimal duct of anuran amphibians: suggestions on its functional role in vomeronasal perception. J Anat 216:510–517PubMedCrossRefGoogle Scholar
  12. Paterson NF (1951) The nasal cavities of the toad Hemipipa carvalhoi Mir.-Rib. and other Pipidae. Proc Zool Soc Lond 121:381–415Google Scholar
  13. Reiss JO, Burd GD (1997) Cellular and molecular interactions in the development of the Xenopus olfactory system. Sem Cell Dev Biol 8:171–179CrossRefGoogle Scholar
  14. Reiss JO, Eisthen HL (2008) Chemical senses: comparative anatomy and physiology of chemical senses in amphibians. In: Thewissen JGM, Nummela S (eds) Sensory evolution on the threshold: adaptions in secondarily aquatic vertebrates. University of California Press, California, pp 43–63Google Scholar
  15. Rowedder W (1937) Die Entwicklung des Geruchsorgans bei Alytes obstetricans und Bufo vulgaris. Zeitschr f Anat u Entwicklungsgesch 107:91–123CrossRefGoogle Scholar
  16. Slabbert GK (1945) Contributions to the cranial morphology of the European anuran Bombina variegata (Linné). Ann Univ Stell 23:67–89Google Scholar
  17. Snyder DA, Rivers AM, Yokoe H, Menco BPM, Anholt RRH (1991) Olfactomedin: purification, characterization, and localization of a novel olfactory glycoprotein. Biochemistry 30:9143–9153PubMedCrossRefGoogle Scholar
  18. Trotier T, Døving KB, Rosin JF (1994) Functional properties of frog vomeronasal receptor cells. In: Kurihana K, Suzuki N, Ogawa H (eds) Olfaction and taste XI, Springer. Tokyo, Japan, pp 188–191Google Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Christine Nowack
    • 1
  • Sabrina Jordan
    • 1
  • Carolin Wittmer
    • 1
  1. 1.Department of Zoology/Developmental BiologyUniversity of Kassel, WG Functional Vertebrate AnatomyKasselGermany

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