Abstract
Both SHED and DPSC have characteristic indistinguishable fibroblastic characteristics (Fig 6.1). They preserve plasticity during at least 25 passages, while maintaining the normal karyotype and the rate of expansion characteristic of stem cells (Kerkis et al. 2006).
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Abe S et al (2010) Oral bacterial extracts facilitate early osteogenic/dentinogenic differentiation in human dental pulp-derived cells. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109(1):149–154
Arthur A et al (2008) Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues. Stem Cells 26(7):1787–1795
Arthur A et al (2009) Eph/ephrinB mediate dental pulp stem cell mobilization and function. J Dent Res 88(9):829–834
Bakopoulou A et al (2011) Assessment of the impact of two different isolation methods on the osteo/odontogenic differentiation potential of human dental stem cells derived from deciduous teeth. Calcif Tissue Int 88(2):130–141
Batouli S et al (2003) Comparison of stem-cell-mediated osteogenesis and dentinogenesis. J Dent Res 82(12):976–981
Bayati V et al (2011) The evaluation of cyclic uniaxial strain on myogenic differentiation of adipose-derived stem cells. Tissue Cell 43(6):359–366
Bialek P et al (2004) A twist code determines the onset of osteoblast differentiation. Dev Cell 6(3):423–435
Bonewald LF et al (2003) von Kossa staining alone is not sufficient to confirm that mineralization in vitro represents bone formation. Calcif Tissue Int 72(5):537–547
Cai X et al (2011) Uniaxial cyclic tensile stretch inhibits osteogenic and odontogenic differentiation of human dental pulp stem cells. J Tissue Eng Regen Med 5(5):347–353
Casagrande L et al (2010) Dentin-derived BMP-2 and odontoblast differentiation. J Dent Res 89(6):603–608
Chaussain C et al (2009) MMP2-cleavage of DMP1 generates a bioactive peptide promoting differentiation of dental pulp stem/progenitor cell. Eur Cell Mater 18:84–95
Chen HC et al (2012) MicroRNA and messenger RNA analyses of mesenchymal stem cells derived from teeth and the Wharton jelly of umbilical cord. Stem Cells Dev 21(6):911–922
Chen S et al (2009) Runx2, osx, and dspp in tooth development. J Dent Res 88(10):904–909
Cordeiro MM et al (2008) Dental pulp tissue engineering with stem cells from exfoliated deciduous teeth. J Endod 34(8):962–969
Couble ML et al (2000) Odontoblast differentiation of human dental pulp cells in explant cultures. Calcif Tissue Int 66(2):129–138
d’Aquino R et al (2007) Human postnatal dental pulp cells co-differentiate into osteoblasts and endotheliocytes: a pivotal synergy leading to adult bone tissue formation. Cell Death Differ 14(6):1162–1171
d’Aquino R et al (2011) Human neural crest-derived postnatal cells exhibit remarkable embryonic attributes either in vitro or in vivo. Eur Cell Mater 21:304–316
Dominici M et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8(4):315–317
Duailibi MT et al (2004) Bioengineered teeth from cultured rat tooth bud cells. J Dent Res 83(7):523–528.
Gandia C et al (2008) Human dental pulp stem cells improve left ventricular function, induce angiogenesis, and reduce infarct size in rats with acute myocardial infarction. Stem Cells 26(3):638–645
Govindasamy V et al (2010) Inherent differential propensity of dental pulp stem cells derived from human deciduous and permanent teeth. J Endod 36(9):1504–1515
Govindasamy V et al (2011) Differentiation of dental pulp stem cells into islet-like aggregates. J Dent Res 90(5):646–652
Gronthos S et al (2000) Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci U S A 97(25):13625–13630
Gronthos S et al (2002) Stem cell properties of human dental pulp stem cells. J Dent Res 81(8):531–535
He H et al (2008) Effects of FGF2 and TGFbeta1 on the differentiation of human dental pulp stem cells in vitro. Cell Biol Int 32(7):827–834
Honda MJ et al (2007). Side population cells expressing ABCG2 in human adult dental pulp tissue. Int Endod J 40(12):949–958
Huang GT et al (2009) Mesenchymal stem cells derived from dental tissues vs. those from other sources: their biology and role in regenerative medicine. J Dent Res 88(9):792–806
Huang GT et al (2006) In vitro characterization of human dental pulp cells: various isolation methods and culturing environments. Cell Tissue Res 324(2):225–236
Huang S et al (2007) Bifurcation dynamics in lineage-commitment in bipotent progenitor cells. Dev Biol 305(2):695–713
Iida K et al (2010) Hypoxia enhances colony formation and proliferation but inhibits differentiation of human dental pulp cells. Arch Oral Biol 55(9):648–654
Iohara K et al (2004) Dentin regeneration by dental pulp stem cell therapy with recombinant human bone morphogenetic protein 2. J Dent Res 83(8):590–595
Iohara K et al (2006) Side population cells isolated from porcine dental pulp tissue with self-renewal and multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells 24(11):2493–2503
Ishkitiev N et al (2010) Deciduous and permanent dental pulp mesenchymal cells acquire hepatic morphologic and functional features in vitro. J Endod 36(3):469–474
Kadar K et al (2009) Differentiation potential of stem cells from human dental origin - promise for tissue engineering. J Physiol Pharmacol 60 Suppl 7:167–175
Karahuseyinoglu S et al (2007a) Biology of stem cells in human umbilical cord stroma: in situ and in vitro surveys. Stem Cells 25(2):319–331
Karahuseyinoglu S et al (2008) Functional structure of adipocytes differentiated from human umbilical cord stroma-derived stem cells. Stem Cells 26(3):682–691
Kerkis I, Caplan AI (2012) Stem cells in dental pulp of deciduous teeth. Tissue Eng Part B Rev 18(2):129–138
Kerkis I et al (2006) Isolation and characterization of a population of immature dental pulp stem cells expressing OCT-4 and other embryonic stem cell markers. Cells Tissues Organs 184(3–4):105–116
Kim JK et al (2011) mTor plays an important role in odontoblast differentiation. J Endod 37(8):1081–1085
Kiraly M et al (2011) Integration of neuronally predifferentiated human dental pulp stem cells into rat brain in vivo. Neurochem Int 59(3):371–381
Komori T et al (1997) Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell 89(5):755–764
Komori T (2010) Regulation of bone development and extracellular matrix protein genes by RUNX2. Cell Tissue Res 339(1):189–195
Koyama N et al (2009) Evaluation of pluripotency in human dental pulp cells. J Oral Maxillofac Surg 67(3):501–506
Kronenberg HM (2004) Twist genes regulate Runx2 and bone formation. Dev Cell 6(3):317–318
Laino G et al (2005) A new population of human adult dental pulp stem cells: a useful source of living autologous fibrous bone tissue (LAB). J Bone Miner Res 20(8):1394–1402
Laino G et al (2006) An approachable human adult stem cell source for hard-tissue engineering. J Cell Physiol 206(3):693–701
Lee JY et al (2011) The effects of platelet-rich plasma derived from human umbilical cord blood on the osteogenic differentiation of human dental stem cells. In Vitro Cell Dev Biol Anim 47(2):157–164
Lengner CJ et al (2007) Oct4 expression is not required for mouse somatic stem cell self-renewal. Cell Stem Cell 1(4):403–415
Li Y et al (2011) TWIST1 promotes the odontoblast-like differentiation of dental stem cells. Adv Dent Res 23(3):280–284
Liu H et al (2006) Dental pulp stem cells. Methods Enzymol 419:99–113
Liu L et al (2011) Expression pattern of Oct-4, Sox2, and c-Myc in the primary culture of human dental pulp derived cells. J Endod 37(4):466–472
Lizier NF et al (2012) Scaling-up of dental pulp stem cells isolated from multiple niches. PLoS One 7(6):e39885
Mehrazarin S et al (2011) Impaired odontogenic differentiation of senescent dental mesenchymal stem cells is associated with loss of Bmi-1 expression. J Endod 37(5):662–666
Min JH et al (2011) Dentinogenic potential of human adult dental pulp cells during the extended primary culture. Hum Cell 24(1):43–50
Miyazaki T et al (2008) Inhibition of the terminal differentiation of odontoblasts and their transdifferentiation into osteoblasts in Runx2 transgenic mice. Arch Histol Cytol 71(2):131–146
Nakamura S et al (2009) Stem cell proliferation pathways comparison between human exfoliated deciduous teeth and dental pulp stem cells by gene expression profile from promising dental pulp. J Endod 35(11):1536–1542
Nakashima M et al (2004) Stimulation of reparative dentin formation by ex vivo gene therapy using dental pulp stem cells electrotransfected with growth/differentiation factor 11 (Gdf11). Hum Gene Ther 15(11):1045–1053
Nam S et al (2011) Odontogenic differentiation of human dental pulp stem cells stimulated by the calcium phosphate porous granules. J Tissue Eng 2011:812547
Nourbakhsh N et al (2011) Induced in vitro differentiation of neural-like cells from human exfoliated deciduous teeth-derived stem cells. Int J Dev Biol 55(2):189–195
Ohazama A et al (2004) Stem-cell-based tissue engineering of murine teeth. J Dent Res 83(7):518–522
Okamoto Y et al (2009) Simvastatin induces the odontogenic differentiation of human dental pulp stem cells in vitro and in vivo. J Endod 35(3):367–372
Oktar PA et al (2011) Continual expression throughout the cell cycle and downregulation upon adipogenic differentiation makes nucleostemin a vital human MSC proliferation marker. Stem Cell Rev 7(2):413–424
Osathanon T et al (2011) Basic fibroblast growth factor inhibits mineralization but induces neuronal differentiation by human dental pulp stem cells through a FGFR and PLCgamma signaling pathway. J Cell Biochem 112(7):1807–1816
Paino F et al (2010) Ecto-mesenchymal stem cells from dental pulp are committed to differentiate into active melanocytes. Eur Cell Mater 20:295–305
Papaccio G et al (2006) Long-term cryopreservation of dental pulp stem cells (SBP-DPSCs) and their differentiated osteoblasts: a cell source for tissue repair. J Cell Physiol 208(2):319–325
Pivoriuunas A et al (2010) Proteomic analysis of stromal cells derived from the dental pulp of human exfoliated deciduous teeth. Stem Cells Dev 19(7):1081–1093
Sarraf CE et al (2011) In vitro mesenchymal stem cell differentiation after mechanical stimulation. Cell Prolif 44(1):99–108
Shi S et al (2001) Comparison of human dental pulp and bone marrow stromal stem cells by cDNA microarray analysis. Bone 29(6):532–539
Stevens A et al (2008) Human dental pulp stem cells differentiate into neural crest-derived melanocytes and have label-retaining and sphere-forming abilities. Stem Cells Dev 17(6):1175–1184
Struys T et al (2011) Ultrastructural and immunocytochemical analysis of multilineage differentiated human dental pulp- and umbilical cord-derived mesenchymal stem cells. Cells Tissues Organs 193(6):366–378
Thesleff I et al (1995) Regulation of organogenesis. Common molecular mechanisms regulating the development of teeth and other organs. Int J Dev Biol 39(1):35–50
Wang J et al (2010) The presence of a side population and its marker ABCG2 in human deciduous dental pulp cells. Biochem Biophys Res Commun 400(3):334–339
Wang X et al (2011) Inhibition of Delta1 promotes differentiation of odontoblasts and inhibits proliferation of human dental pulp stem cell in vitro. Arch Oral Biol 56(9):837–845
Wang YH et al (2006) Examination of mineralized nodule formation in living osteoblastic cultures using fluorescent dyes. Biotechnol Prog 22(6):1697–1701
Yamada Y et al (2006) A feasibility of useful cell-based therapy by bone regeneration with deciduous tooth stem cells, dental pulp stem cells, or bone-marrow-derived mesenchymal stem cells for clinical study using tissue engineering technology. Tissue Eng Part A 16(6):1891–1900
Yang B et al (2012) Tooth root regeneration using dental follicle cell sheets in combination with a dentin matrix - based scaffold. Biomaterials 33(8):2449–2461
Yang X et al (2007) The odontogenic potential of STRO-1 sorted rat dental pulp stem cells in vitro. J Tissue Eng Regen Med 1(1):66–73
Yang X et al (2009) Mineralized tissue formation by BMP2-transfected pulp stem cells. J Dent Res 88(11):1020–1025
Yildirim S et al (2012) Diff erentiation potentials of two stroma-resident tissue-specifi c stem cells. Niche Niche, Journal of Cellular Therapy and Regenerative Medicine (in Press)
Yildirim S et al (2008) The role of dental pulp cells in resorption of deciduous teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105(1):113–120
Yu F et al (2006a) Drosophila neuroblast asymmetric cell division: recent advances and implications for stem cell biology. Neuron 51(1):13–20
Yu J et al (2010) Differentiation potential of STRO-1+ dental pulp stem cells changes during cell passaging. BMC Cell Biol 11:32
Yu J et al (2006b) Probing gene expression in live cells, one protein molecule at a time. Science 311(5767):1600–1603
Yu V et al (2009) Dynamic hydrostatic pressure promotes differentiation of human dental pulp stem cells. Biochem Biophys Res Commun 386(4):661–665
Zhang H et al (2012) Natural mineralized scaffolds promote the dentinogenic potential of dental pulp stem cells via the mitogen-activated protein kinase signaling pathway. Tissue Eng Part A 18(7–8):677–691
Zhou JX et al (2011) Predicting pancreas cell fate decisions and reprogramming with a hierarchical multi-attractor model. PLoS One 6(3):e14752
Author information
Authors and Affiliations
Rights and permissions
Copyright information
© 2013 Author
About this chapter
Cite this chapter
Yildirim, S. (2013). Characterization of DPSC. In: Dental Pulp Stem Cells. SpringerBriefs in Stem Cells. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-5687-2_6
Download citation
DOI: https://doi.org/10.1007/978-1-4614-5687-2_6
Published:
Publisher Name: Springer, New York, NY
Print ISBN: 978-1-4614-5686-5
Online ISBN: 978-1-4614-5687-2
eBook Packages: Biomedical and Life SciencesBiomedical and Life Sciences (R0)