Skip to main content
SpringerLink
Log in
Book cover

Proteomics in Foods pp 205–222Cite as

Fish Authentication

  • Chapter
  • First Online:

Part of the book series: Food Microbiology and Food Safety ((RESDEV,volume 2))

Abstract

Fish are among the most internationally traded food commodities. However, due to the globalization of markets, illegal species substitution is becoming an important concern. Traditionally, the identification of fish relied on morphological analysis but this is infeasible in the case of processed products. Although over the last two decades, several DNA and protein molecular markers have been developed, a new strategy for fish authentication is emerging due to the recent developments in the proteomics methodologies. This chapter is devoted to the presentation of a comprehensive overview of the application of proteomics to fish species authentication. Two consecutive phases of the proteomics pipeline used in our laboratory (discovery and target-driven stages) are presented and emphasized using as an example the identification of all commercial fish species belonging to the Merlucciidae family. Moreover, the fastest method described to date (<2 h) for species authentication in food products, based on the monitoring of species-specific peptide biomarkers by LC-MS/MS in the SMIM mode, is illustrated as an example. Future outlooks and perspectives are provided as well.

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

References

  • Aebersold R, Mann M (2003) Mass spectrometry-based proteomics. Nature 422:198–207

    Article  CAS  Google Scholar 

  • AOAC (1990) Official method of analysis. In: Association of official analytical chemists, Helrich, K. (Ed.). 15th Edn., vol. 2, Arlington, Virginia, USA

    Google Scholar 

  • Appel RD, Bairoch A, Sanchez JC, Vargas JR, Golaz O, Pasquali C, Hochstrasser DF (1996) Federated 2-DE database: a simple means of publishing 2-DE data. Electrophoresis 17:540–546

    Article  CAS  Google Scholar 

  • Aranishi F, Okimoto T, Izumi S (2005) Identification of gadoid species (Pisces, Gadidae) by PCR-RFLP analysis. J Appl Genet 46:69–73

    Google Scholar 

  • Asensio L, González I, Fernández A, Rodríguez MA, Lobo E, Hernández PE, García T, Martín R (2002) Application of random amplified polymorphic DNA (RAPD) analysis for identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. J Food Prot 65:432–435

    CAS  Google Scholar 

  • Asensio L, González I, Rodríguez MA, Mayoral B, López-Calleja I, Hernández PE, García T, Martín R (2003a) Identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets by polyclonal antibody-based enzyme linked immunosorbent assay. J Agric Food Chem 51:1169–1172

    Article  CAS  Google Scholar 

  • Asensio L, González I, Rodríguez MA, Mayoral B, López-Calleja I, Hernández PE, García T, Martín R (2003b) Development of a specific monoclonal antibody for grouper (Epinephelus guaza) identification by an indirect enzyme-linked immunosorbent assay. J Food Prot 66:886–889

    CAS  Google Scholar 

  • Asensio L, González I, Rodríguez MA, Hernández PE, García T, Martín R (2004) PCR-ELISA for the semiquantitative detection of Nile perch (Lates niloticus) in sterilized fish muscle mixtures. J Agric Food Chem 52:4419–4422

    Article  CAS  Google Scholar 

  • Berrini A, Tepedino V, Borromeo V, Secchi C (2006) Identification of freshwater fish commercially labelled “perch” by isoelectric focusing and two-dimensional electrophoresis. Food Chem 96:163–168

    Article  CAS  Google Scholar 

  • Böhme K, Fernández-No IC, Barros-Velázquez J, Gallardo JM, Cañas B, Calo-Mata P (2011) Rapid species identification of seafood spoilage and pathogenic Gram-positive bacteria by MALDI-TOF mass fingerprinting. Electrophoresis 32:2951–2965

    Article  Google Scholar 

  • Calo-Mata P, Sotelo CG, Pérez-Martín RI, Rehbein H, Hold GL, Russell VJ, Pryde S, Quinteiro J, Rey-Méndez M, Rosa C, Santos AT (2003) Identification of gadoid fish species using DNA-based techniques. Eur Food Res Technol 217:259–264

    Article  CAS  Google Scholar 

  • Carrera M (2008) Proteómica bottom-up y top-down de organismos poco secuenciados. Secuenciación de novo de péptidos biomarcadores para la identificación de especies de la familia Merlucciidae. Tesis Doctoral, Vigo University

    Google Scholar 

  • Carrera M, Cañas B, Piñeiro C, Vázquez J, Gallardo JM (2006) Identification of commercial hake and grenadier species by proteomic analysis of the parvalbumin fraction. Proteomics 6:5278–5287

    Article  CAS  Google Scholar 

  • Carrera M, Cañas B, Piñeiro C, Vázquez J, Gallardo JM (2007) De novo mass spectrometry sequencing and characterization of species-specific peptides from nucleoside diphosphate kinase B for the classification of commercial fish species belonging to the family Merlucciidae. J Proteome Res 6:3070–3080

    Article  CAS  Google Scholar 

  • Carrera M, Barros L, Cañas B, Gallardo JM (2009) Discrimination of South African commercial fish species (Merluccius capensis and Merluccius paradoxus) by LC-MS/MS analysis of the protein aldolase. J Aquat Food Prod Tech 18:67–78

    Article  CAS  Google Scholar 

  • Carrera M, Cañas B, Vázquez J, Gallardo JM (2010) Extensive de novo sequencing of new parvalbumin isoforms using a novel combination of bottom-up proteomics, accurate molecular mass measurement by FTICR-MS and selected MS/MS ion monitoring. J Proteome Res 9:4393–4406

    Article  CAS  Google Scholar 

  • Carrera M, Cañas B, López-Ferrer D, Piñeiro C, Vázquez J, Gallardo JM (2011) Fast monitoring of species-specific peptide biomarkers using high-intensity-focused-ultrasound-assisted tryptic digestion and selected MS/MS ion monitoring. Anal Chem 83:5688–5695

    Article  CAS  Google Scholar 

  • Castillo AG, Martínez JL, García-Vázquez E (2003) Identification of Atlantic hake species by a simple PCR-based methodology employing microsatellite loci. J Food Prot 66:2130–2134

    CAS  Google Scholar 

  • Céspedes A, García T, Carrera E, González I, Fernández A, Asensio L, Hernández PE, Martín R (1999a) Indirect enzyme-linked immunosorbent assay for the identification of sole (Solea solea), European plaice (Pleuronectes platessa), flounder (Platichthys flesus), and Greenland halibut (Reinhardtius hippoglossoides). J Food Prot 62:1178–1182

    Google Scholar 

  • Céspedes A, García T, Carrera E, González I, Fernández A, Hernández PE, Martín R (1999b) Identification of sole (Solea solea) and Greenland halibut (Reinhardtius hippoglossoides) by PCR amplification of the 5S rDNA gene. J Agric Food Chem 47:1046–1050

    Article  Google Scholar 

  • Chapela MJ, Sánchez A, Suárez MI, Pérez-Martín RI, Sotelo CG (2007) A rapid methodology for screening hake species (Merluccius spp.) by single-stranded conformation polymorphism analysis. J Agric Food Chem 55:6903–6909

    Article  CAS  Google Scholar 

  • Chen TY, Shiau CY, Wei CI, Hwang DF (2004) Preliminary study on puffer fish proteome species identification of puffer fish by two-dimensional electrophoresis. J Agric Food Chem 52:2236–2241

    Article  CAS  Google Scholar 

  • Chen S, Zhang J, Chen W, Xu D, Zhou Y (2011) Species identification of grouper and snapper in Taiwan Strait using polymerase chain reaction-restriction fragment length polymorphism analysis and lab-on-a-chip system. Se Pu 29:677–680

    CAS  Google Scholar 

  • Colombo F, Mangiagalli G, Renon P (2005) Identification of tuna species by computer-assisted and cluster analysis of PCR-SSCP electrophoretic patterns. Food Control 16:51–53

    Article  CAS  Google Scholar 

  • Comesaña AS, Abella P, Sanjuan A (2003) Molecular identification of five commercial flatfish species by PCR-RFLP analysis of a 12S rRNA gene fragment. J Sci Food Agric 83:752–759

    Article  Google Scholar 

  • Council Regulation (EC) No 104/2000 of 17 December 1999 on the common organization of the markets in fishery and aquaculture products. Off J Eur Com L 17:22

    Google Scholar 

  • Council Regulation (EC) No 2065/2001 of 22 October 2001 laying down detailed rules for the application of Council Regulation (EC) No 104/2000 as regards informing consumers about fishery and aquaculture products. Off J Eur Com, L:278, 6

    Google Scholar 

  • Domínguez E, Pérez MD, Puyol P, Calvo M (1997) Use of immunological techniques for detecting species substitution in raw and smoked fish. Z Lebensm Unters Forsch A 204:279–281

    Article  Google Scholar 

  • Elsayed S, Bennich H (1975) The primary structure of allergen M from cod. Scand J Immunol 4:203–208

    Article  CAS  Google Scholar 

  • Eng JK, McCormack AL, Yates JR III (1994) An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. J Am Soc Mass Spectrom 5:976–989

    Article  CAS  Google Scholar 

  • FAO, SOFIA (2010) The state of world fisheries and aquaculture 2010. ISSN 1020–5489

    Google Scholar 

  • FAO yearbook (2000) Fishery statistics. Catches. Vol 86/1

    Google Scholar 

  • Gajewski KG, Chen YT, Hsieh YH (2009) Production and characterization of monoclonal antibodies specific to pangasius catfish, basa and tra. J Food Sci 74:C241–C247

    Article  CAS  Google Scholar 

  • Guidance for Industry (2012) The seafood list – FDA’s guide to acceptable market names for seafood sold in interstate commerce

    Google Scholar 

  • Hooper L, Thompson RL, Harrison RA, Summerbell CD, Ness AR, Moore HJ, Worthington HV, Durrington PN, Higgins JP, Capps NE, Riemersma RA, Ebrahim SB, Davey SG (2006) Risk and benefits of omega 3 fats for mortality, cardiovascular disease, and cancer: systematic review. Br Med J 332:752–760

    Article  CAS  Google Scholar 

  • Huang TS, Marshall MR, Kuo KJ, Otwell WS, Wei CI (1995) Development of monoclonal antibodies for red snapper (Lutjanus campechanus) identification using enzyme linked immunosorbent assay. J Agric Food Chem 43:2301–2307

    Article  CAS  Google Scholar 

  • Jérôme M, Lemaire C, Verrez-Bagnis V, Etienne M (2003) Direct sequencing method for species identification of canned sardine and sardine-type products. J Agric Food Chem 51:7326–7332

    Article  Google Scholar 

  • Kawai Y, Uematsu S, Shinano H (1992) Effect of heat-treatment on some physicochemical properties and emulsifying activity of carp sarcoplasmic protein. Nippon Suisan Gakkai 58:1327–1331

    Article  Google Scholar 

  • Keenan CP, Shaklee JB (1985) Electrophoretic identification of raw and cooked fish fillets and other marine products. Food Tech Australia 37:117–128

    CAS  Google Scholar 

  • Klose J (1975) Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals. Humangenetik 26:231–243

    CAS  Google Scholar 

  • Kochzius M, Nölte M, Weber H, Silkenbeumer N, Hjörleifsdottir S, Hreggvidsson GO, Marteinsson V, Kappel K, Planes S, Tinti F, Magoulas A, Garcia-Vázquez E, Turan C, Hervet C, Campo Falgueras D, Antoniou A, Landi M, Blohm D (2008) DNA microarrays for identifying fishes. Mar Biotechnol 10:207–217

    Article  CAS  Google Scholar 

  • López JL, Marina A, Álvarez G, Vázquez J (2002) Application of proteomics for fast identification of species-specific peptides from marine species. Proteomics 2:1658–1665

    Article  Google Scholar 

  • Ma B, Zhang K, Hendrie C, Liang C, Li M, Doherty-Kirby A, Lajoie G (2003) PEAKS: powerful software for peptide de novo sequencing by tandem mass spectrometry. Rapid Commun Mass Spectrom 17:2337–2342

    Article  CAS  Google Scholar 

  • Mackie I, Craig A, Etienne M, Jérôme M, Fleurence J, Jessen F, Smelt A, Kruijt A, Malmheden Yman I, Ferm M, Martinez I, Pérez-Martín R, Piñeiro C, Rehbein H, Kündiger R (2000) Species identification of smoked and gravad fish products by sodium dodecylsulphate polyacrylamide gel electrophoresis, urea isoelectric focusing and native isoelectric focusing: a collaborative study. Food Chem 71:1–7

    Article  CAS  Google Scholar 

  • Mairs DF, Sindermann CJ (1962) A serological comparison of five species of Atlantic clupeoid fishes. Biol Bull 123:330–343

    Article  Google Scholar 

  • Maldini M, Marzano FN, Fortes GG, Papa R, Gandolfi G (2006) Fish and seafood traceability based on AFLP markers: elaboration of a species database. Aquaculture 261:487–494

    Article  CAS  Google Scholar 

  • Martinez I, Jakobsen Friis TJ (2004) Application of proteome analysis to seafood authentication. Proteomics 4:347–354

    Article  CAS  Google Scholar 

  • Martinez I, Jakobsen Friis T, Seppola M (2001) Requirements for the application of protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis and randomly amplified polymorphic DNA analyses to product speciation. Electrophoresis 22:1526–1533

    Article  CAS  Google Scholar 

  • Mazzeo MF, de Giulio B, Guerriero G, Ciarcia G, Malorni A, Russo GL, Siciliano RA (2008) Fish authentication by MALDI-TOF mass spectrometry. J Agric Food Chem 56:11071–11076

    Article  CAS  Google Scholar 

  • McNulty ST, Klesius PH (2005) Development of an indirect enzyme-linked immunoabsorbent assay using a monoclonal antibody to identify Ictalurus sp. fillets. Aquacult Res 36:1279–1284

    Article  Google Scholar 

  • O’Farrell PH (1975) High resolution two-dimensional electrophoresis of proteins. J Biol Chem 250:4007–4021

    Google Scholar 

  • Ortea I, Cañas B, Gallardo JM (2009) Mass spectrometry characterization of species-specific peptides from arginine kinase for the identification of commercially relevant shrimp species. J Proteome Res 8:5356–5362

    Article  CAS  Google Scholar 

  • Pandey A, Mann M (2000) Proteomics to study genes and genomes. Nature 405:837–846

    Article  CAS  Google Scholar 

  • Partis L, Wells RJ (1996) Identification of fish species using random amplified polymorphic DNA (RAPD). Mol Cell Probes 10:435–441

    Article  CAS  Google Scholar 

  • Pérez J, García-Vázquez E (2004) Genetic identification of nine hake species for detection of commercial fraud. J Food Prot 67:2792–2796

    Google Scholar 

  • Pérez M, Álarez C, Balado M, Cabado AG, Vieites JM, Presa P (2004) Identification of South Atlantic hakes (Merluccius australis and Merluccius hubbsi) in processed foods by PCR-RFLPs of cytochrome b gene. J Aquat Food Product Technol 13:59–67

    Article  Google Scholar 

  • Piggot GM (1976) New approaches to marketing fish. In: Altschul AM (ed) New protein foods. Academic, New York, pp 1–37

    Google Scholar 

  • Piñeiro C, Barros-Velázquez J, Sotelo CG, Pérez-Martín RI, Gallardo JM (1998) Two dimensional electrophoretic study of the water-soluble protein fraction in white muscle of gadoid fish species. J Agric Food Chem 46:3991–3997

    Article  Google Scholar 

  • Piñeiro C, Barros-Velázquez J, Pérez-Martín RI, Martínez I, Jacobsen T, Rehbein H, Kündiger R, Mendes R, Etienne M, Jerome M, Craig A, Mackie IM, Jessen F (1999a) Development of a sodium dodecyl sulfate-polyacrylamide gel electrophoresis reference method for the analysis and identification of fish species in raw and heat-processed samples: a collaborative study. Electrophoresis 20:1425–1432

    Article  Google Scholar 

  • Piñeiro C, Barros-Velázquez J, Sotelo CG, Gallardo JM (1999b) The use of two dimensional electrophoresis in the characterization of the water-soluble protein fraction of commercial flat fish species. Z Lebensm Unters Forsch A 208:342–348

    Article  Google Scholar 

  • Piñeiro C, Barros-Velázquez J, Pérez-Martín RI, Gallardo JM (2000) Specific enzyme detection following isoelectric focusing as a complimentary tool for the differentiation of related Gadoid fish species. Food Chem 70:241–245

    Article  Google Scholar 

  • Piñeiro C, Vázquez J, Marina AI, Barros-Velázquez J, Gallardo JM (2001) Characterization and partial sequencing of species-specific sarcoplasmic polypeptides from commercial hake species by mass spectrometry following two-dimensional electrophoresis. Electrophoresis 22:1545–1552

    Article  Google Scholar 

  • Quinteiro J, Vidal R, Izquierdo M, Sotelo CG, Chapela MJ, Pérez-Martín RI, Rehbein H, Hold GL, Russel VJ, Pryde SE, Rosa C, Santos AT, Rey-Méndez M (2001) Identification of hake species (Merluccius genus) using sequencing and PCR-RFLP analysis of mitochondrial DNA control region sequences. J Agric Food Chem 49:5108–5114

    Article  CAS  Google Scholar 

  • Rasmussen RS, Morrissey MT (2009) Application of DNA-based methods to identify fish and seafood substitution on the commercial market. Compr Rev Food Sci Food Safety 8:118–154

    Article  CAS  Google Scholar 

  • Regulatory Fish Encyclopedia, FDA (2011) (http://www.fda.gov/Food/FoodSafety/Product-SpecificInformation/Seafood/RegulatoryFishEncyclopediaRFE/ucm219129.htm)

  • Rehbein H, Kress G, Schmidt T (1997) Application of PCR-SSCP to species identification of fishery products. J Sci Food Agric 74:35–41

    Article  CAS  Google Scholar 

  • Rehbein H, Kündiger R, Pineiro C, Perez-Martin RI (2000) Fish muscle parvalbumins as marker proteins for native and urea isoelectric focusing. Electrophoresis 21:1458–1463

    Article  CAS  Google Scholar 

  • Royal Decree 1380/2002 of Dec 20 (BOE No. 3, 3 Jan 2003) from Ministry of Agriculture, Fisheries and Food, Spain

    Google Scholar 

  • Royal Decree 1702/2004 of Jul 16 (BOE No. 172, 17 Jul 2004) from Ministry of Agriculture, Fisheries and Food, Spain

    Google Scholar 

  • Royal Decree 121/2004 of Jan 23 (BOE No. 31, 5 Feb 2004) from Ministry of Agriculture, Fisheries and Food, Spain

    Google Scholar 

  • Russell VJ, Hold GL, Pryde SE, Rehbein H, Quinteiro J, Rey-Méndez M, Sotelo CG, Pérez-Martín RI, Santos AT, Rosa C (2000) Use of restriction fragment length polymorphism to distinguish between salmon species. J Agric Food Chem 48:2184–2188

    Article  CAS  Google Scholar 

  • Sánchez A, Quinteiro J, Rey-Méndez M, Pérez-Martín RI, Sotelo CG (2009) Identification of European hake species (Merluccius merluccius) using real-time PCR. J Agric Food Chem 57:3397–3403

    Article  Google Scholar 

  • Santaclara FJ, Cabado AG, Vieites JM (2006) Development of a method for genetic identification of four species of anchovies: E. encrasicolus, E. anchoita, E. ringens and E. japonicus. Eur Food Res Technol 223:609–614

    Article  CAS  Google Scholar 

  • Scigelova M, Maroto F, Dufresne C, Vázquez J (2007) High-throughput de novo sequencing. http://www.thermo.com/

  • Scobbie AE, Mackie IM (1988) The use of sodium dodecylsulphate polyacrylamide gel electrophoresis is fish species identification -a procedure suitable for cooked and raw fish. J Sci Food Agricul 44:343–351

    Article  CAS  Google Scholar 

  • Sicherer SH, Sampson HA (2010) Food allergy. J Allergy Clin Immunol 125(2 Suppl 2):S116–S125

    Article  Google Scholar 

  • Sotelo CG, Pérez-Martín RI (2007) Fish and seafood authentication. In: Authentication of food and wine (SE Ebeler, GR Takeoka, P Winterhalter, eds.), pp. 126–137. ACS Symposium Series 952, Washington (USA)

    Google Scholar 

  • Sotelo CG, Calo-Mata P, Chapela MJ, Pérez-Martín RI, Rehbein H, Hold GL, Russell VJ, Pryde S, Quinteiro J, Izquierdo M, Rey-Méndez M, Rosa C, Santos AT (2001) Identification of flatfish (Pleuronectiforme) species using DNA-based techniques. J Agric Food Chem 49:4562–4569

    Article  CAS  Google Scholar 

  • Tepedino V, Berrini A, Borromeo V, Gaggioli D, Cantoni C, Manzoni P, Secchi C (2001) Identification of commercial fish species belonging to the orders pleuronectiformes and gadiformes: library of isoelectric focusing patterns. J AOAC Int 84:1600–1607

    CAS  Google Scholar 

  • Trotta M, Schönhuth S, Pepe T, Cortesi ML, Puyet A, Bautista JM (2005) Multiplex PCR method for use in real-time PCR for identification of fish fillets from grouper (Epinephelus and Mycteroperca species) and common substitute species. J Agric Food Chem 53:2039–2045

    Article  CAS  Google Scholar 

  • Zhang Q, Rasco BA (1996) Quantitative Western blotting method for determining the species of origin in surimi. IFT annual meeting: book of abstracts. p. 146. ISSN 1082–1236

    Google Scholar 

  • Zhang J, Huang H, Cai Z, Huang L (2006) Species identification in salted products of red snappers by semi-nested PCR-RFLP based on the mitochondrial 12S rRNA gene sequence. Food Control 17:557–563

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular Systems Biology (IMSB), ETH Zürich, Wolfgang-Pauli-Str, Zürich, 16 8093, Switzerland

    Mónica Carrera

  2. Complutense University of Madrid, Madrid, Spain

    Benito Cañas

  3. Marine Research Institute, CSIC, Vigo, Pontevedra, Spain

    José M. Gallardo

Authors
  1. Mónica Carrera
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Benito Cañas
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. José M. Gallardo
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Mónica Carrera .

Editor information

Editors and Affiliations

  1. , Department of Food Science, Instituto de Agroquimica y Tecnologia de, Avenue Agustin Escardino 7, Paterna, 46980, Valencia, Spain

    Fidel Toldrá

  2. , Department of Applied Engineering Scienc, University College Ghent, Schoonmeersstraat 52, Gent, B9000, Belgium

    Leo M. L. Nollet

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this chapter

Cite this chapter

Carrera, M., Cañas, B., Gallardo, J.M. (2013). Fish Authentication. In: Toldrá, F., Nollet, L. (eds) Proteomics in Foods. Food Microbiology and Food Safety, vol 2. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-5626-1_12

Download citation

Publish with us

Policies and ethics

Search

Navigation