Abstract
Inflammatory molecules (IMs) play an important role in ionizing radiation (IR)-induced soft tissue damage. The alteration of IMs as a function of time was studied with a protein array containing 62 IMs in mouse cutaneous soft tissues exposed to 30 Gy. The results showed that: (1) 2 days after irradiation, the levels of TGF-β1, MIP-1γ, IL-1α, and sTNF RI increased, while IGFBP-3, CXCL16, and IL-1β decreased in IR skin as compared to control skin; (2) 21 days after IR, TGF-β1, and MIP-1 γ, IL-1α remained high, while CXCL16 and IL-1β remained low; (3) 3 months after IR, the cytokine pattern exhibited reversals. The levels of MIP-1γ decreased, while VCAM-1, IGFBP-3, and TGF-β1 production increased. The data indicated that: (a) IMs change as a function of time after soft tissue irradiation; (b) changing IM levels may reflect the altered balance of the cytokine network, leading to imbalance or homeostasis; and (c) an antibody-based protein array can be used to assess multiple IMs simultaneously, making it useful for bulk screening for changes in tissue cytokine levels.
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Acknowledgments
This project is supported in part by U19 AI067733. We thank Kate Casey-Sawicki for editing this manuscript.
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Xiao, Z. et al. (2013). Alteration of the Inflammatory Molecule Network After Irradiation of Soft Tissue. In: Welch, W.J., Palm, F., Bruley, D.F., Harrison, D.K. (eds) Oxygen Transport to Tissue XXXIV. Advances in Experimental Medicine and Biology, vol 765. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4989-8_47
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DOI: https://doi.org/10.1007/978-1-4614-4989-8_47
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