In the wake of the 9/11 terrorist attacks and the recent Level 7 nuclear event at the Fukushima Daiichi plant, there has been heightened awareness of the possibility of radiological terrorism and accidents and the need for techniques to estimate radiation levels after such events. A number of approaches to monitoring radiation using biological markers have been published, including physical techniques, cytogenetic approaches, and direct, DNA-analysis approaches. Each approach has the potential to provide information that may be applied to the triage of an exposed population, but problems with development and application of devices or lengthy analyses limit their potential for widespread application. We present a post-irradiation observation with the potential for development into a rapid point-of-care device. Using simple mitochondrial proteomic analysis, we investigated irradiated and nonirradiated murine mitochondria and identified a protein mobility shift occurring at 2–3 Gy. We discuss the implications of this finding both in terms of possible mechanisms and potential applications in bio-radiation monitoring.
Mitochondrial proteomic analysis Radiation
This is a preview of subscription content, log in to check access
We thank Kate Casey-Sawicki for editing and preparing this manuscript for publication.
Zhang H, Zhang SB, Sun W et al (2009) B1 sequence-based real-time quantitative PCR: a sensitive method for direct measurement of mouse plasma DNA levels after gamma irradiation. Int J Radiat Oncol Biol Phys 74:1592–1599CrossRefPubMedPubMedCentralGoogle Scholar
Zhang H, Maguire DJ, Zhang M et al (2011) Elevated mitochondrial DNA copy number and POL-gamma expression but decreased expression of TFAM in murine intestine following therapeutic dose irradiation. Adv Exp Med Biol 701:201–206CrossRefPubMedPubMedCentralGoogle Scholar
Suzuki H, Tamukai K, Yoshida N et al (2010) Development of a compact electron spin resonance system for measuring ESR signals of irradiated fingernails. Health Phys 98:318–321CrossRefPubMedGoogle Scholar
Black PJ, Swarts SG (2010) Ex vivo analysis of irradiated fingernails: chemical yields and properties of radiation-induced and mechanically-induced radicals. Health Phys 98:301–308CrossRefPubMedPubMedCentralGoogle Scholar
Romanyukha A, Reyes RA, Trompier F et al (2010) Fingernail dosimetry: current status and perspectives. Health Phys 98:296–300CrossRefPubMedGoogle Scholar
Sharma M, Halligan BD, Wakim BT et al (2008) The urine proteome as a biomarker of radiation injury: submitted to proteomics – clinical applications special issue: “renal and urinary proteomics (Thongboonkerd)”. Proteomics Clin Appl 2:1065–1086CrossRefPubMedPubMedCentralGoogle Scholar
Chen C, Boylan MT, Evans CA et al (2005) Application of two-dimensional difference gel electrophoresis to studying bone marrow macrophages and their in vivo responses to ionizing radiation. J Proteome Res 4:1371–1380CrossRefPubMedGoogle Scholar
Szkanderova S, Vavrova J, Hernychova L et al (2005) Proteome alterations in gamma-irradiated human T-lymphocyte leukemia cells. Radiat Res 163:307–315CrossRefPubMedGoogle Scholar
Guipaud O, Holler V, Buard V et al (2007) Time-course analysis of mouse serum proteome changes following exposure of the skin to ionizing radiation. Proteomics 7:3992–4002CrossRefPubMedGoogle Scholar
Menard C, Johann D, Lowenthal M et al (2006) Discovering clinical biomarkers of ionizing radiation exposure with serum proteomic analysis. Cancer Res 66:1844–1850CrossRefPubMedGoogle Scholar
Romm H, Wilkins RC, Coleman CN et al (2011) Biological dosimetry by the triage dicentric chromosome assay: potential implications for treatment of acute radiation syndrome in radiological mass casualties. Radiat Res 175:397–404CrossRefPubMedGoogle Scholar
Schagger H, von Jagow G (1991) Blue native electrophoresis for isolation of membrane protein complexes in enzymatically active form. Anal Biochem 199:223–231CrossRefPubMedGoogle Scholar