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Chemical Properties of Propolis Collected by Stingless Bees

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Pot-Honey

Abstract

The aim of this study is to determine the chemical composition of propolis collected by stingless bees. For this purpose eight ethanol extracts of propolis collected by Melipona favosa from Venezuela, Melipona grandis, Scaptotrigona depilis, and Scaptotrigona polysticta from Bolivia, and Tetragonula biroi from Philippines were prepared and analyzed. According to gas chromatography–mass spectrometry (GC–MS) results, aliphatic acids and their esters, alcohols, aromatic acids and their esters, hydrocarbons and terpenes were identified from propolis samples. All three Venezuelan propolis include clay. Bolivian propolis include more components than propolis from the Philippines. We found kaur-16-ene (8 beta,13 beta), olean-12-ene, 3-keto-urs-12-ene in propolis collected by stingless bees, as new compounds observed only in Bolivian propolis.

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References

  • Bankova V. 2005. Chemical diversity of propolis and the proplem of standardization. Journal of Ethnopharmacology 100:114–117.

    Article  PubMed  CAS  Google Scholar 

  • Barth OM, Luz CF. 2003. Palynological analysis of Brazilian geopropolis sediments. Grana 42:121–127.

    Google Scholar 

  • Barth OM. 2004. Melissopalynology in Brazil: a review of pollen analysis of honeys, propolis and pollen loads of bees. Scientia Agricola 61:342–350.

    Article  Google Scholar 

  • Barth OM. 2006. Palynological analysis of geopropolis samples obtained from six species of Meliponinae in the Campus of the Universidade de Ribeirao Preto, USP, Brazil. Apiacta 41:71–85.

    Google Scholar 

  • Burdock GA. 1998. Review of the biological properties and toxicity of bee propolis (propolis). Food and Chemical Toxicology 36:347–363.

    Article  PubMed  CAS  Google Scholar 

  • Evans JD, Aronstein K, Chen YP, Hetru C, Imler J, Jiang H, Kanost M, Thompson GJ, Zou Z, Hultmark D. 2006. Immune pathways and defence mechanisms in honey bees Apis mellifera. Insect Molecular Biology 15:645–656.

    Article  PubMed  CAS  Google Scholar 

  • Farnesi AP, Aquino-Ferreira R, De Jong D, Bastos JK, Soares AE. 2009. Effects of stingless bee and honey bee propolis on four species of bacteria. Genetics and Molecular Research 8:635–640.

    Article  CAS  Google Scholar 

  • Finstrom MS, Spivak M. 2010. Propolis and bee health: the natural history and significance of resin use by honey bees. Apidologie 41:295–311.

    Article  Google Scholar 

  • Freitas MO, Ponte FAF, Lima MAS, Silveria ER. 2008. Flavonoids and triterpenes from the nest of the stingless bee Trigona spinipes. Journal of Brazilian Chemical Society 19:532–535.

    Article  CAS  Google Scholar 

  • Gençay Ö, Salih B. 2009. GC-MS analysis of propolis samples from 17 different regions of Turkey, four different regions of Brazil and one from Japan. Mellifera 9:19–28.

    Google Scholar 

  • Ghisalberti EL. 1979. Propolis: a review. Bee World 60:59–84.

    CAS  Google Scholar 

  • Greenaway W, Scaysbrook T, Whatley FR. 1987. The analysis of bud exudate of Populus x euramericana, and of propolis, by gas chromatography-mass spectrometry. Prooceeding of the Royal Society London B 232:249–272.

    Article  CAS  Google Scholar 

  • Greenaway W, Scaysbrook T, Whatley FR. 1990. The composition and plant origins of propolis: a report of work at Oxford. Bee World 71:107–118.

    Google Scholar 

  • Heard TA. 1999. The role of stingless bees in crop pollination. Annual Review of Entomology 44:183–206.

    Google Scholar 

  • Krol W, Scheller S, Shani J, Pietsz G, Czuba Z. 1993. Synergistic effect of ethanolic extract of propolis and antibiotics on the growth of Staphylococcus aureus. Arzneimittelforschung 43:607–609.

    PubMed  CAS  Google Scholar 

  • Langenheim JH, Lincoln DE, Foster CE, Stubblebine WH. 1978. Implications of variation in resin compoistion among organs, tissues and population in tropical legume Hymenaea. Biochemical Systematics and Ecology 4:299–313.

    Google Scholar 

  • Leonhardt SD, Blüthgen N. 2009. A sticky affair: resin collection by Bornean stingless bees. Biotropica 41:730–736.

    Article  Google Scholar 

  • Leonhardt SD, Blüthgen N, Schmitt T. 2009. Smelling like resin: terpenoids account for species-specific cuticular profiles in Southeast-Asian stingless bees. Insectes Sociaux 56:157–170.

    Article  Google Scholar 

  • Leonhardt SD, Zeilhofer S, Blüthgen N, Schmitt T. 2010. Stingless bees use terpenes as olfactory cues to find resin sources. Chemical Senses 35:603–611.

    Article  PubMed  CAS  Google Scholar 

  • Leonhardt SD, Wallace HM, Schmitt T. 2011. The cuticular profiles of Australian stingless bees are sahped by resin of eucalypt tree Corymbia torelliana. Austral Ecology 36:537–543.

    Article  Google Scholar 

  • Marcucci MC, Ferreres F, Garcia-Viguera C, Bankova VS, De Castro SL, Dantas AP, Valente PHM, Paulino N. 2001. Phenolic compounds from Brazilian propolis with pharmacological activities. Journal of Ethnopharmacology 74:105–112.

    Article  PubMed  CAS  Google Scholar 

  • Massaro FC, Brooks PR, Wallace HM, Russell FD. 2011. Cerumen of Australian stingless bees (Tetragonula carbonaria): gas chromatography-mass spectrometry fingerprints and potential anti-inflammatory properties. Naturwissenschaften 98:329–337.

    Google Scholar 

  • Patricio EFLRA, Lopez LC, Maile R, Tentschert J, Jones GR, Morgan ED. 2002. The propolis of stingless bees: terpenes from the tibia of three Frieseomelitta species. Journal of Insect Physiology 48:249–254.

    Article  PubMed  CAS  Google Scholar 

  • Pereira AS, Bicalho B, Neto FR. 2003. Comparison of propolis from Apis mellifera and Tetragonisca angustula. Apidologie 34:291–298.

    Article  Google Scholar 

  • Ricciardelli D’Albore G. 1979. L’origine geographique de la propolis. Apidologie 10:241–267.

    Article  Google Scholar 

  • Roubik D. 2006. Stingless bee nesting biology. Apidologie 37:124–143.

    Article  Google Scholar 

  • Sforcin JM, Fernandes A, Lopes CAM, Bankova V, Funari SRC. 2000. Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethnopharmacology 73:242–249.

    Article  Google Scholar 

  • Sforcin JM, Bankova V. 2011. Propolis: is there a potential for the development of new drugs? Journal of Ethnophatmacology 133:253–260.

    Article  CAS  Google Scholar 

  • Simone M, Spivak M. 2010. Propolis and bee health: the natural history and significance of resin use by honey bees. Apidologie 41:295–311.

    Article  Google Scholar 

  • Singer RB, Flach A, Koehler S, Marsaioli AJ, Amaral MC. 2004. Sexual mimicry in Mormolyca rigens (Lindl.) Schltr. (Orchidaceae: Maxillariinae). Annals of Botany 93:755–762.

    Article  PubMed  Google Scholar 

  • Tomás-Barberán FA, García-Viguera C, Vit-Olivier P, Ferreres F, Tomás-Lorente F. 1993. Phytochemical evidence for the botanical origin of tropical propolis from Venezuela. Phytochemistry 34:191–196.

    Article  Google Scholar 

  • Torres A, Garedew A, Schmolz E, Lamprecht I. 2004. Calorimetric investigation of the antimicrobial action and insight into the chemical properties of “angelita” honey—a product of the stingless bee Tetragonisca angustula from Colombia. Thermochimica Acta 415:107–113.

    Article  CAS  Google Scholar 

  • Velikova M, Bankova V, Tsvetko I, Kujumgiev A, Marcucci MC. 2000. Antibacterial ent-kaurene from Brazilian propolis of native stingless bees. Fitoterapia 71:693–696.

    Article  PubMed  CAS  Google Scholar 

  • Vit P, Medina M, Enriquez ME. Quality standards for medicinal uses of Meliponinae honey in Guatemala, Mexico and Venezuela. Bee World 85:2–5.

    Google Scholar 

  • Wallace HM, Trueman SJ. 1995. Dispersal of Eucalyptus torelliana seeds by the resin-collecting stingless bee, Trigona carbonaria. Oecologia 104:12–16.

    Article  Google Scholar 

  • Wallace HM, Lee DJ. 2010. Resin-foraging by colonies of Trigona sapiens and T. hockingsi (Hymenoptera: Apidae, Meliponini) and consequent seed dispersal of Corymbia torelliana (Myrtaceae). Apidologie 41:428–435.

    Article  Google Scholar 

  • Warakomska Z, Maciejewicz W. 1992. Microscopic analysis of propolis from Polish regions. Apidologie 23:277–283.

    Article  Google Scholar 

Download references

Acknowledgements

The propolis samples were received from the collection of Apiterapia y Bioactividad (APIBA), Universidad de Los Andes, Mérida, Venezuela, seeking for collaborative research. The M. favosa propolis were collected by Professor Patricia Vit, Universidad de Los Andes, Mérida, Venezuela, and the bee was kindly identified by Prof. João M.F. Camargo from the Biology Department, Universidade de São Paulo, Ribeirão Preto, Brazil. The propolis of T. biroi was collected by Professor Cleofás Cervancia, Universidad Los Baños, Philippine, and she also identified the bee. The M. grandis, S. depilis and S. polysticta propolis from the National Park Amboró, Bolivia were collected by P. Vit and Dr. Urbelinda Ferrufino, Asociación Ecológica de Oriente (ASEO), Santa Cruz, Bolivia. The Bolivian stingless bees were kindly identified by Dr. Silvia R.M. Pedro from the Biology Department, Universidade de São Paulo, Ribeirão Preto, Brazil. I thank the anonymous reviewers to improve my chapter, as well as valued editorial interaction with P. Vit and Dr. David W. Roubik.

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Correspondence to Ömür Gençay Çelemli .

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Çelemli, Ö.G. (2013). Chemical Properties of Propolis Collected by Stingless Bees. In: Vit, P., Pedro, S., Roubik, D. (eds) Pot-Honey. Springer, New York, NY. https://doi.org/10.1007/978-1-4614-4960-7_39

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