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Pot-Honey pp 495-505 | Cite as

Bioactivity of Honey and Propolis of Tetragonula laeviceps in Thailand

  • Chanpen ChanchaoEmail author
Chapter
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Abstract

In Thailand, bee products have long been used in traditional medicine. Honey and propolis extracts of Tetragonula laeviceps inhibit the growth of many microorganisms, including methicillin-resistant Staphylococcus aureus (by honey) and Herpes simplex virus. The chemical composition of water extracts of propolis (WEP) and dose-dependent antimicrobial activities are reported. Ethanol extract of propolis (EEP) has antimalarial activity, as do peptides. Both honey and propolis may possess anticancer qualities. The percentage of cell viability, DNA fragmentation, and change in morphology of treated cancer cells are described. Safety and efficacy of chemical raw materials, including contamination with plant pollen and pathogenic spores, must be addressed before advocating widespread consumption of raw bee products.

Keywords

Antimicrobial Peptide Royal Jelly Micrococcus Luteus Propolis Extract Honey Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Notes

Acknowledgments

I wish to thank the following: the Thailand Research Fund (grant # RMU5180042); the National Research Council of Thailand; the Asahi Glass Foundation; the Thai Government Stimulus Package 2 (TKK2555), under the Project for the Establishment of a Comprehensive Center for Innovative Food, Health Products and Agriculture; the Ratchadapisek Somphot Endowment Fund (AG001B); and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, for financial support. I also thank Dr. Orawan Duangphakdee, King Mongkut’s University of Technology Thonburi, Ratchaburi Campus, Bangkok, Thailand for honey collection. Finally, I express my gratitude to Professor Patricia Vit for the invitation to write this chapter, and all the editorial support received from her and Dr. David W Roubik.

References

  1. Aburahma SK, Khader YS, Alzoubi K, Sawalha N. 2010. Complementary and alternative medicine use in a pediatric neurology clinic. Complementary Therapies in Clinical Practice 16:117–120.PubMedCrossRefGoogle Scholar
  2. Akiyasu T, Paudyal B, Paudyal P, Kumiko M, Kazue U, Takuji N, Takashi K, Yoshihisa N, Minoru K. 2010. A case report of acute renal failure associated with bee pollen contained in nutritional supplements. Therapeutic Apheresis and Dialysis 14:93–97.PubMedCrossRefGoogle Scholar
  3. Antúnez K, D’Alessandro B, Piccini C, Corbella E, Zunino P. 2004. Paenibacillus larvae larvae spores in honey samples from Uruguay: a nationwide survey. Journal of Invertebrate Pathology 86:56–58.PubMedCrossRefGoogle Scholar
  4. Boonsai P. 2009. Sugar, flavonoid contents and paper chromatography of propolis extracts from Apis mellifera and Trigona laeviceps. Senior research report, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.Google Scholar
  5. Boukraa L, Sulaiman SA. 2009. Rediscovering the antibiotics of the hive. Recent Patents on Anti-Infective Drug Discovery 4:206–213.PubMedCrossRefGoogle Scholar
  6. Butnariu MV, Giuchici CV. 2011. The use of some nanoemulsions based on aqueous propolis and lycopene extract in the skin’s protective mechanisms against UVA radiation. Journal of Nanobiotechnology 9:3.PubMedCrossRefGoogle Scholar
  7. Cagli KE, Tufekcioglu O, Sen N, Aras D, Topaloglu S, Basar N, Pehlivan S. 2009. Atrioventricular block induced by mad-honey intoxication. Texas Heart Institute Journal 36:342–344.PubMedGoogle Scholar
  8. Casteels-Josson K, Capaci T, Casteels P, Tempst P (1993) Apidaecin multipeptide precursor structure: a putative mechanism for amplification of the insect antibacterial response. EMBO J 12:1569–1578.Google Scholar
  9. Chanchao C, Teethakaew T, Wongsiri S. 2006. Alpha-glucosidase activity in honey of Apis spp. in Thailand. Laos Journal on Applied Science 1:276–281.Google Scholar
  10. Chanchao C. 2009. Antimicrobial activity by Trigona laeviceps (stingless bee) honey from Thailand. Pakistan Journal of Medical Sciences 25:364–369.Google Scholar
  11. Chartthai C. 2010. Antimicrobial activity on pathogenic bacteria by Trigona laeviceps honey extracts. Senior research report, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.Google Scholar
  12. Dubey L, Maskey A, Regmi S. 2009. Bradycardia and severe hypotension caused by wild honey poisoning. Hellenic Journal of Cardiology 50:426–428.Google Scholar
  13. Epand RM, Vogel HJ (1999) Diversity of antimicrobial peptides and their mechanisms of action. BBA –Biomembranes 1462:11–28.Google Scholar
  14. Gulcin T, Bursal E, Şehitoĝlu MH, Bilsel M, Goren AC (2010) Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food and Chemical Toxicology 48:2227–2238.Google Scholar
  15. Guney A, Karaman I, Oner M, Yerer MB. 2011. Effects of propolis on fracture healing: anexperimental study. Phytotherapy Research as doi:  10.1002/ptr.3470.
  16. Jirakanwisal K. 2010. Antimicrobial activity on methicillin-resistant Staphylococcus aureus by Trigona laeviceps honey extracts. Senior research report, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.Google Scholar
  17. Kaewwongwattana P. 2009. Total protein content and major protein subunits in propolis extract from Apis mellifera and Trigona laeviceps. Senior research report, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.Google Scholar
  18. Kaewmuangmoon J, Nonthapa P, Rattanawannee A, Winayanuwattikun P, Chanchao C (2012) Preliminary screening for various bioactivities in honey and propolis extracts from Thai bees. EJMP 2:74–92.Google Scholar
  19. Koca I, Koca AF. 2007. Poisoning by mad honey: a brief review. Food and Chemical Toxicology 45:1315–1318.PubMedCrossRefGoogle Scholar
  20. Kwakman PH, Zaat SA. 2011. Antibacterial components of honey. The International Union of Biochemistry and Molecular Biology Life as doi:  10.1002/iub.578.
  21. Kwakman PH, de Boer L, Ruyter-Spira CP, Creemers-Molenaar T, Helsper JP, Vandenbroucke-Grauls CM, Zaat SA, te Velde AA. 2011a. Medical-grade honey enriched with antimicrobial peptides has enhanced activity against antibiotic-resistant pathogens. European Journal of Clinical Microbiology and Infectious Diseases 30:251–257.CrossRefGoogle Scholar
  22. Kwakman PH, te Velde AA, de Boer L, Vandenbroucke-Grauls CM, Zaat SA. 2011b. Two major medicinal honeys have different mechanisms of bactericidal activity. PLoS ONE as doi:  10.1371/journal.pone.0017709.
  23. Langenheim JH. 2003. Plant resins. Chemistry, evolution, ecology, ethnobotany. Timber Press; Portland, Oregon, USA. 586 pp.Google Scholar
  24. Li F, Awale S, Tezuka Y, Esumi H, Kadota S. 2010. Study on the constituents of Mexican propolis and their cytotoxic activity against PANC-1 human pancreatic cancer cells. Journal of Natural Products 73:623–627.PubMedCrossRefGoogle Scholar
  25. Litwin A, Flanagan M, Entis G, Gottschlich G, Esch R, Gartside P, Michael JG. 1997. Oral immunotherapy with short ragweed extract in a novel encapsulated preparation: a double-blind study. Journal of Allergy and Clinical Immunology 100:30–38.PubMedCrossRefGoogle Scholar
  26. Miguel MG, Nunes S, Dandlen SSA, Cavaco AM, Antunes MD. 2010. Antioxidant activity of propolis from algarve. Advances in Environmental Biology 5:345–350.Google Scholar
  27. Okuyan E, Uslu A, Ozan Levent M. 2010. Cardiac effects of “mad honey”: a case series.Clinical Toxicology 48:528–532.PubMedCrossRefGoogle Scholar
  28. Olofsson TC, Vásquez A. 2008. Detection and identification of a novel lactic acid bacterial flora within the honey stomach of the honeybee Apis mellifera. Current Microbiology 57:356–363.PubMedCrossRefGoogle Scholar
  29. Paul IM, Beiler J, McMonagle A, Shaffer ML, Duda L, Berlin CM Jr. 2007. Effect of honey, dextromethorphan, and no treatment on nocturnal cough and sleep quality for coughing children and their parents. Archives of Pediatrics and Adolescent Medicine161:1140–1146.PubMedCrossRefGoogle Scholar
  30. Phuapradit W, Saropala N. 1992. Topical application of honey in treatment of abdominal wound disruption. Australian and New Zealand Journal of Obstetrics and Gynaecology 32:381–384.PubMedCrossRefGoogle Scholar
  31. Phuapradit W. 2002. Honey and sugar as a dressing for wounds and ulcers. Tropical Doctor 32:55.PubMedGoogle Scholar
  32. Piccini C, D’Alessandro B, Antúnez K, Zunino P. 2002. Detection of Paenibacillus larvae subspecies larvae spores in naturally infected bee larvae and artificially contaminated honey by PCR. World Journal of Microbiology and Biotechnology 18:761–765.CrossRefGoogle Scholar
  33. Postmes T, van den Bogaard AE, Hazen M. 1995. The sterilization of honey with cobalt 60 gamma radiation: a study of honey spiked with spores of Clostridium botulinum and Bacillus subtilis. Experientia 51:986–989.PubMedCrossRefGoogle Scholar
  34. Riches H. 2000. Medical aspects of beekeeping. HR Books; Northwood. 86 pp.Google Scholar
  35. Salomão K, Pereira PR, Campos LC, Borba CM, Cabello PH, Marcucci MC, de Castro SL. 2008. Brazilian propolis: correlation between chemical composition and antimicrobial activity. Evidence-Based Complementary and Alternative Medicine 5:317–324.PubMedCrossRefGoogle Scholar
  36. Saxena P, Pant VA, Wadhwani KK, Kashyap MP, Gupta SK, Pant AB. 2011. Potential of the propolis as storage medium to preserve the viability of cultured human periodontal ligament cells: an in vitro study. Dental Traumatology 27:102–108.PubMedCrossRefGoogle Scholar
  37. Shadkam MN, Mozaffari-Khosravi H, Mozayan MR. 2010. A comparison of the effect of honey, dextromethorphan and diphenhydramine on nightly cough and sleep quality in children and their parents. Journal of Alternative and Complementary Medicine 16:787–793.CrossRefGoogle Scholar
  38. Tasaniyananda N. 2010. Effect of Trigona laeviceps honey extracts on antiproliferation of cancer cell lines. Senior research report, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.Google Scholar
  39. Teixeira EW, Message D, Negri G, Salatino A, Stringheta PC. 2010. Seasonal variation, chemical composition and antioxidant activity of Brazilian propolis samples. Evidence-Based Complementary and Alternative Medicine 7:307–315.PubMedCrossRefGoogle Scholar
  40. Thai Ministry of Public Health during 2005–2009. Available at: http://bps.ops.moph.go.th/2.3.4-52.pdf
  41. Ting PT, Silver S. 2004. Allergic contact dermatitis to propolis. Journal of Drugs in Dermatology 3:685–686.PubMedGoogle Scholar
  42. Tomás-Barberán FA, Ferreres F, Amparo Blazquez M, García-Viquera C, Tomas-Lorente F. 1993. High-performance liquid chromatography of honey flavonoids. Journal of Chromatography 634:41–46.CrossRefGoogle Scholar
  43. Umthong S, Puthong S, Chanchao C. 2009. Trigona laeviceps propolis from Thailand: antimicrobial, antiproliferative and cytotoxic activities. The American Journal of Chinese Medicine 37:855–865.PubMedCrossRefGoogle Scholar
  44. Umthong S, Phuwapraisirisan P, Puthong S, Chanchao C. 2011. In vitro antiproliferative activity of partially purified Trigona laeviceps propolis from Thailand on human cancer cell lines. BMC Complementary and Alternative Medicine. doi:  10.1186/1472-6882-11-37.
  45. Viljakainen L, Evans JD, Hasselmann M, Rueppell O, Tingek S, Pamilo P (2009) Rapid evolution of immune proteins in social insects. Molecular Biology and Evolution 26:1791–1801.Google Scholar
  46. Wannakul P (2007) Growth inhibition of microorganisms by Apis dorsata honey. Senior research report, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.Google Scholar
  47. Wongchum N. 2007. Growth inhibition of microorganisms by Trigona laeviceps (stingless bee) honey. Senior research report, Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok, Thailand.Google Scholar
  48. Yoshiyama M, Kimura K. 2010. Characterization of antimicrobial peptide genes from Japanese honeybee Apis cerana japonica (Hymenoptera: Apidae). Japanese Journal of Applied Entomology and Zoology 45:609–614.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of BiologyChulalongkorn UniversityBangkokThailand

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