Nanovisualization of Proteins in Action Using High-Speed AFM

Chapter
Part of the Biophysics for the Life Sciences book series (BIOPHYS, volume 2)

Abstract

Direct and real-time visualization of single protein molecules is a powerful approach to understanding how they operate to function. Recent advances in high-speed atomic force microscopy (HS-AFM) provide a new opportunity to visualize dynamic events of label-free proteins in action under physiological conditions, at subsecond temporal and submolecular resolution. In this chapter, we first overview HS-AFM techniques used for fast and low-invasive imaging of proteins. Then, we highlight recent imaging studies on myosin V walking on an actin filament, rotary catalysis of rotorless F1-ATPase, and processive run of cellulase hydrolyzing cellulose fibers.

Keywords

Cellulose Hydrolysis Graphite Phenol Torque 

Notes

Acknowledgments

The authors thank Daisuke Yamamoto, Hayato Yamashita, Mikihiro Shibata, Ryota Iino, Hiroyuki Noji, Kiyohiko Igarashi, Hideki Kandori, and many present and past students in Ando lab for collaborations or contributions to the work described in this chapter. Long-term financial support by NEDO, JST (CREST project), JSPS (Grant-in-Aid for Basic Research (S)), Knowledge Cluster Initiative, and the Mitsubishi Foundation is gratefully acknowledged.

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Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Takayuki Uchihashi
    • 1
  • Noriyuki Kodera
    • 2
  • Toshio Ando
    • 1
  1. 1.Department of Physics and Bio-AFM Frontier Research CenterKanazawa UniversityKanawazaJapan
  2. 2.Bio-AFM Frontier Research CenterKanazawa UniversityKanawazaJapan

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