Breeding and Biotechnology of Jatropha curcas

  • Daniela de Argollo Marques
  • Walter José Siqueira
  • Carlos Augusto Colombo
  • Roseli Aparecida Ferrari
Chapter

Abstract

The growing demand for renewable energy sources will increase the demand for oilseeds in Brazil and worldwide. This demand will only be supplied if plant varieties with exceptional agronomic and chemical traits are developed. This process can be accelerated by application of biotechnology and molecular biology techniques, as we have done in the case of Jatropha curcas, a perennial oilseed. We have used two strategies: (1) Exploitation of pre-existing variability in the germplasm of J. curcas evaluated through morphological, molecular and chemical markers (such as fatty acid and phorbol esters content). We also investigated self-fertilization with selected genotypes to analyze the effects of inbreeding, particularly with regard to the production of non-toxic, smaller, male-sterile and gynoic plants; and (2) Increase the genetic variability through interspecific hybridization and biotechnology. Despite the post-zygotic incompatibility encountered in most interspecific crosses, the use of artificial hybridization coupled with tissue culture techniques, such as in vitro embryo rescue and subsequent multiplication via somatic embryogenesis has yielded unprecedented results in the form of rare hybrids between J. curcas and other species of this genus. So far, we have obtained more than 80 F1 hybrids and 140 BC1 × F1 backcross progenies. Evaluation of these hybrids and the continuation of this line of research will greatly expand the germplasm bank of Instituto Agronômico de Capinas (IAC) and should contribute significantly to rapid production and release of new improved cultivars. In addition to their application in assessing genetic variability, molecular markers were useful to confirm F1 hybrids and BC1 generations by excluding the possibility of apomixis and to select suitable plants for genetic breeding.

Keywords

Biomass Toxicity Corn Petroleum Recombination 

Abbreviations

AFLP

Amplified fragment length polymorphism

cTBP

Combinatorial tubulin-based polymorphism

IAC

Instituto Agronômico, Campinas, São Paulo, Brasil (Agronomic Institute), São Paulo, Brazil

ISSR

Inter-simple sequence repeat

ITAL

Instituto de Tecnologia dos Alimentos (Food Technology Institute) São Paulo Brazil

PCR

Polymerase chain reaction

RAPD

Random amplified polymorphic DNA

RT-PCR

Real-time reverse transcription PCR

SPAR

Simple primer amplification reactions

SSR

Simple sequence repeat

Notes

Acknowledgements

The authors thank Petrobras for financial support and fellowships provided to students and trainees at Instituto Agronômico de Campinas (IAC) and Instituto de Tecnologia dos Alimentos (ITAL).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Daniela de Argollo Marques
    • 1
  • Walter José Siqueira
    • 1
  • Carlos Augusto Colombo
    • 1
  • Roseli Aparecida Ferrari
    • 2
  1. 1.Centro de Pesquisa e Desenvolvimento de Recursos Genéticos VegetaisInstituto Agronômico de Campinas (IAC)Campinas/São PauloBrazil
  2. 2.Centro de Ciência e Qualidade de AlimentosInstituto de Tecnologia dos Alimentos (ITAL)CampinasBrazil

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