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Sodium-Calcium Exchanger Modulates the L-Glutamate Cai2+ Signalling in Type-1 Cerebellar Astrocytes

  • Héctor Rojas
  • Claudia Colina
  • Magaly Ramos
  • Gustavo Benaim
  • Erica Jaffe
  • Carlo Caputo
  • Reinaldo Di PoloEmail author
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 961)

Abstract

We have previously demonstrated that rat type-1 cerebellar astrocytes express a very active Na+/Ca2+ exchanger which accounts for most of the total plasma membrane Ca2+ fluxes and for the clearance of Ca i 2+ induced by physiological agonist. In this chapter, we have explored the mechanism by which the reverse Na+/Ca2+ exchange is involved in agonist-induced Ca2+ signalling in rat cerebellar astrocytes. Laser-scanning confocal microscopy experiments using immunofluorescence labelling of Na+/Ca2+ exchanger and RyRs demonstrated that they are highly co-localized. The most important finding presented in this chapter is that L-glutamate activates the reverse mode of the Na+/Ca2+ exchange by inducing a Na+ entry through the electrogenic Na+-glutamate co-transporter and not through the ionophoric L-glutamate receptors as confirmed by pharmacological experiments with specific blockers of ionophoric L-glutamate receptors, electrogenic glutamate transporters and the Na/Ca exchange.

Keywords

Na+/Ca2+ exchange CICR Glutamate Glutamate transporter 

Notes

Acknowledgements

This work was supported by FONACIT-Venezuela (G-0010000637).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Héctor Rojas
    • 1
  • Claudia Colina
    • 1
  • Magaly Ramos
    • 1
  • Gustavo Benaim
    • 2
  • Erica Jaffe
    • 1
  • Carlo Caputo
    • 1
  • Reinaldo Di Polo
    • 1
    Email author
  1. 1.Laboratorio de Fisiología CelularCentro de Biofísica, Instituto Venezolano de Investigaciones Científicas (IVIC)CaracasVenezuela
  2. 2.Laboratorio de Señalización CelularInstituto de Estudios Avanzados (IDEA)CaracasVenezuela

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