The Nonhuman Primates as Models for Studying Human Atherosclerosis: Studies on the Chimpanzee, the Baboon and the Rhesus Macacus

  • V. Blaton
  • H. Peeters
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 67)


This work was supported by grants from the National Institutes of Health, Bethesda, Md (USA), Public Health Service Grant no HE9969, the World Health Organization, the NFGWO (Belgium) Grants no 1153, 1206, the Franqui Foundation and the RUCA-ZOO (Antwerpen).


Nonhuman Primate Plasma Lipoprotein World Monkey Spider Monkey High Cholesterol Diet 
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  1. 1.
    Clarkson, T.B. (1972). Animal models of atherosclerosis. In: Advances in Veterinary Science and Comparative Science, ed. C.A. Brandly and C.E. Cornelius, Academic Press, New York, pp. 151–173.Google Scholar
  2. 2.
    Portman, O., and S.B. Andrus (1965). Comparative evaluation of three species of New World Monkeys for studies of dietary factors, tissue lipids and atherogenesis. J. Nutr., 87, 429–438.PubMedGoogle Scholar
  3. 3.
    Middleton, C.C., T.B. Clarkson, H.B. Lofland and R.W. Prichard (1967). Diet and atherosclerosis of squirrel monkeys. Arch. Pathol., 83, 145–153.PubMedGoogle Scholar
  4. 4.
    Mann, G.V., S.B. Andrus, A. McNally and F.J. Stare (1953). Experimental atherosclerosis in cebus monkeys. J. Exp. Med., 98, 195–218.PubMedCrossRefGoogle Scholar
  5. 5.
    Wissler, R.W., L.E. Frazier, and R.A. Rasmussen (1962). Atherogenesis in the cebus monkey: I. A comparison of three food fats under controlled dietary conditions. Arch. Pathol., 74, 312–322.PubMedGoogle Scholar
  6. 6.
    Bullock, B.C., T.B. Clarkson, N.D. Lehner, H.B. Lofland and R.W. St. Clair (1969). Atherosclerosis in cebus albifrons monkeys. III. Clinical and pathological studies. Exp. Mol. Pathol., 10, 39–62.PubMedCrossRefGoogle Scholar
  7. 7.
    Blaton, V., R. Vercaemst, N. Vandecasteele, H. Caster and H. Peeters (1974). Isolation and partial characterization of chimpanzee plasma high density lipoproteins and their apolipoproteins. Biochemistry, 13, 1127–1135.PubMedCrossRefGoogle Scholar
  8. 8.
    Blaton, V., D. Vandamme, M. Vastesaeger, J. Mortelmans and H. Peeters (1974). Dietary induced hyperbetalipoproteinemia in chimpanzees: comparison to the human hyperlipoproteinemia. Exp. Mol. Pathol., 20, 132–146.PubMedCrossRefGoogle Scholar
  9. 9.
    Vastesaeger, M., V. Blaton, B. Declercq, J. Vercruysse, D. Vandamme, H. Peeters and J. Mortelmans (1973). Comparative treatment of hyperlipoproteinemias in chimpanzees. Acta Zool. Antverpiensia, nr 53, 81.Google Scholar
  10. 10.
    Strong, J.P., and H.C. McGill (1967). Diet and experimental atherosclerosis in baboons. Am. J. Pathol., 50, 669–690.PubMedGoogle Scholar
  11. 11.
    Blaton, V., A.N. Howard, G.A. Gresham, D. Vandamme, and H. Peeters (1970). Lipid changes in the plasma lipoproteins of baboons given an atherogenic diet. I. Changes in the lipids of total plasma and of alpha and beta lipoproteins. Atherosclerosis, 11, 497–507.PubMedCrossRefGoogle Scholar
  12. 12.
    Peeters, H., V. Blaton, B. Declercq, A.N. Howard, and G.A. Gresham (1970). Lipid changes in the plasma lipoproteins of baboons given an atherogenic diet. II. Changes in the phospholipid classes of total plasma and of alpha and beta lipoproteins. Atherosclerosis, 12, 283–290.PubMedCrossRefGoogle Scholar
  13. 13.
    Howard, A.N., V. Blaton, D. Vandamme, N. Van Landschoot, and H. Peeters (1972). Lipid changes in the plasma lipoproteins of baboons given an atherogenic diet. III. A comparison between the plasma of the baboon and chimpanzee given atherogenic diets and lipid changes in human plasma lipoproteins of type II hyperlipoproteinemia. Atherosclerosis, 16, 257–272.PubMedCrossRefGoogle Scholar
  14. 14.
    Manning, P.J. and T.B. Clarkson (1972). Distribution and lipid content of diet-induced atherosclerotic lesions of rhesus monkeys. Exp. Mol. Pathol., 17, 38–54.PubMedCrossRefGoogle Scholar
  15. 15.
    Newman, W.P., C.C. Middleton, T.B. Calrkson, J. Rosal and J.P. Strong (1974). Naturally occurring arterial lesions in New World Primates. Am. Med. Assoc, 98, 173–176.Google Scholar
  16. 16.
    Middleton, C.C, J. Rosal, T.B. Clarkson, W.P. Newman and H.C. Mc.Gill (1967). Arterial lesions in squirrel monkeys Arch. Pathol., 83, 352–358.Google Scholar
  17. 17.
    Clarkson, T.B., H.B. Lofland, B.C. Bullock, N.D. Lehner, R.W. St. Clair and R.W. Prichard (1969). Atherosclerosis in some species of New World Monkeys Ann. N.Y. Acad. Sci., 162, 103–109.CrossRefGoogle Scholar
  18. 18.
    Lofland, H.B., R.W. St. Clair, J.C. McNintch and R.W. Prichard (1967). Atherosclerosis in New World Primates Arch. Pathol., 83, 211–214.Google Scholar
  19. 19.
    Pucak, G.J., N.D. Lehner, T.B. Clarkson, B.C. Bullock and H.B. Lofland (1973). Spider Monkeys (Ateles sp.) as animal models for atherosclerosis research. Exp. Mol. Pathol., 18, 32–49.PubMedCrossRefGoogle Scholar
  20. 20.
    Goodall, J. (1963). My life among wild chimpanzees. Nat. Geographic, 124.Google Scholar
  21. 21.
    Vastesaeger, M.M., and R. Delcourt (1961). Spontaneous atherosclerosis and diet in captive animals. Nutr. Diet, 3, 174–177.Google Scholar
  22. 22.
    Stare, F.J., S.B. Andrus, and R. Ostwald (1963). Primates in medical research with special reference to new world monkeys. In: Proc. Conf. on Research with Primates, Tektronix Foundation, Beaverton, Wash., 59.Google Scholar
  23. 23.
    Manning, G.W. (1942). Coronary disease in the ape. Am. Heart J., 23, 719–724.CrossRefGoogle Scholar
  24. 24.
    Vastesaeger, M.M., and R. Delcourt (1966). Spontaneous atherosclerosis in chimpanzees Acta Cardiol., 11, 283.Google Scholar
  25. 25.
    Peeters, H., and V. Blaton (1972). Comparative lipid values of human and non human primates. In: Medical Primatology III. Ed. M. Jankowski and G. Goldsmith, Karger, Basel, 336–342.Google Scholar
  26. 26.
    Delcourt, R., G. Nissane, P. Osterrieth, and M. Vastesaeger (1964). Acta Cardiol., 19, 531.PubMedGoogle Scholar
  27. 27.
    Keys, A., J.J. Anderson, F. Fidanza, M.H. Keys, and B. Swahn (1955). Effects of diet on blood lipids in man. Clin. Chem., 1, 34.PubMedGoogle Scholar
  28. 28.
    Peeters, H. (1959). Paper electrophoresis: Principles and techniques Adv. Clin. Chem., 2, 2–134.Google Scholar
  29. 29.
    Mann, G.V., S.B. Andrus, A. McNally, and F.J. Stare (1963). Diet and cholesterolemia in chimpanzees. Fed. Proc., 22, 642.Google Scholar
  30. 30.
    Morris, M.D., and W.E. Geer (1970). Familial hyperbetalipoproteinemia in the rhesus monkey. In: Atherosclerosis, ed. R. Jones, Springer Verlag, N.Y., 192.Google Scholar
  31. 31.
    Vastesaeger, M.M., J. Vercruysse and J.J. Martin (1972). Pitfalls of experimental atherosclerosis in the chimpanzee In: Medical Primatology III Ed. M. Jankowski and G. Goldsmith.Google Scholar
  32. 32.
    Lindsay, S., and I.L. Haikoff (1966). Naturally occurring arteriosclerosis in non human primates. J. Atheroscler. Res., 6, 36–61.CrossRefGoogle Scholar
  33. 33.
    Scott, R.F., E.S. Morrison, J. Larnolych, S.C. Nam and F. Carlstron (1967). Experimental atherosclerosis in rhesus monkeys. I. Gross and microscopic features and lipid values in serum and aorta. Exp. Mol. Pathol., 7, 11–33.PubMedCrossRefGoogle Scholar
  34. 34.
    Wissler, R.W., R.H. Hughes, L.E. Frazier, G.S. Getz, and D. Turner (1965). Aortic lesions and blood lipids in rhesus monkeys fed “table prepared: human diets” Circulation, 32, 220–222.Google Scholar
  35. 35.
    Lindsay, S., and I.L. Chaikoff (1957). Atherosclerosis in the baboon. Arch. Pathol., 63, 460–471.Google Scholar
  36. 36.
    McGill, H.C., J.P. Strong, R.L. Holman, and N.T. Werthessen (1960). Arterial lesions in the kenya baboon. Circ. Res. 8, 670–679.CrossRefGoogle Scholar
  37. 37.
    Peeters, H., and V. Blaton (1969). Comparison of lipid and lipoprotein patterns in primates. Acta Zool. et Pathol. Antverpiensia, 48, 221–231.Google Scholar
  38. 38.
    Blaton, V., R. Vercaemst, N. Vinaimont, and H. Peeters (1975). Isolation and characterization of baboon plasma high density lipoproteins and their apolipoproteins: Comparison to human and non human primates. J. Biol. Chem., in press.Google Scholar
  39. 39.
    Gresham, G.A., and G.A. Howard (1965). Vascular lesions in primates. Ann. N.Y. Acad. Sci., 127, 694–701.PubMedCrossRefGoogle Scholar
  40. 40.
    Strong, J.P., and H.C. McGill (1967). Diet and experimental atheroslcerosis in baboons Am. J. Pathol., 50, 669–690.Google Scholar
  41. 41.
    Blaton, V., and H. Peeters (1969). Lipid and fatty acid modifications in plasma lipoproteins of baboons under atherogenic diet. Acta Zool. et Pathol. Antverpiensia, 48, 223–242.Google Scholar
  42. 42.
    Blaton, V., D. Vandamme, and H. Peeters (1972). Chimpanzee and baboon as biochemical models for human atherosclerosis. Medical Primatology III. Ed. M. Jankowski and G. Goldsmith, Karger, Basel, 306–312.Google Scholar
  43. 43.
    Blaton, V., and H. Peeters (1971). Integrated approach to plasma lipid and lipoprotein analysis In: Blood Lipids and Lipoproteins, Ed. G. Nelson, J. Wiley and Sons, New York, 369–431.Google Scholar
  44. 44.
    Howard, A.N., V. Blaton, G.A. Gresham, D. Vandamme, and H. Peeters (1971). The lipoproteins in hyperlipidaemic primates as a model for human atherosclerosis. Protides of the Biological Fluids, 19, 341–344, Pergamn Press.Google Scholar
  45. 45.
    Gresham, G.A., A.N. Howard, J. McQueen, and D.E. Bowyer (1965). Atherosclerosis in primates. Brit. J. Exp. Path., 66, 94–103.Google Scholar
  46. 46.
    Mortelmans, J. (1969). Tranquilization and anaesthesia. In: Primates in Medicine, Ed. W. Beveridge, Karger, 2, 113-122.Google Scholar
  47. 47.
    Blaton, V., H. Peeters, W. De Keersgieter, B. Declercq, D. Depickere, and D. Vandamme (1965). Differential fatty acid composition of alpha 1 and beta lipoproteins. Protides of the Biological Fluids, 13, 315–319, Elsevier.Google Scholar
  48. 48.
    Walton, K.W., and P.J. Scott (1964). Estimation of the low density lipoproteins of serum in health and disease using large molecular weight dextran sulphate. J. Clin. Pathol., 17, 627–643.PubMedCrossRefGoogle Scholar
  49. 49.
    Peeters, H., and E. Laga (1962). Influence des mucopolysaccharides sur la résorption des graisses par le tube digestif. 3e Rencontre sur les Lipides Alimentaires, Rimini, 825-839. ed. La Nutrizione, Roma.Google Scholar
  50. 50.
    De La Huerga, J., C. Yesininck, and H. Popper (1953). Estimation of total serum lipids by a turbidimetric method. Am. J. Clin. Pathol., 23, 1163–1167.Google Scholar
  51. 51.
    Rozenthal, H.L., M.L. Pfluke, and G. Buscaglia (1957). A stable iron reagent for determination of cholesterol. J. Lab. Clin. Med., 50, 318–322.Google Scholar
  52. 52.
    Van Handel, E., and D.B. Zilversmit (1957). Micromethod for the direct determination of serum triglycerides. J. Lab. Clin. Med., 50, 152–157.Google Scholar
  53. 53.
    Sodhi, H.S., Gordon R. Gould (1967). Combination of delipidized high density lipoproteins with lipids. J. Biol. Chem., 242, 1205–1210.PubMedGoogle Scholar
  54. 54.
    Koga, S., L. Bolis, and A.M. Scanu (1971). Isolation and characterisation of subunit polypeptides from apoproteins of rat serum lipoproteins. Biochim. Biophys. Acta, 236, 416–430.PubMedCrossRefGoogle Scholar
  55. 55.
    Lindgren, F.T., and L.C. Jensen (1972). The isolation and quantitative analysis of serum lipoproteins In: Blood Lipids and Lipoproteins, Ed. G. Nelson, J. Wiley and Sons, New York, 181–274.Google Scholar
  56. 56.
    Van Melsen, A., Y. De Greve, F. Vanderveiken, M. Vastesaeger, V. Blaton, and H. Peeters (1974). A modified method for phenotyping of hyperlipoproteinemia on agarose electrophoresis. Clin. Chim. Acta, 55, 225–234.PubMedCrossRefGoogle Scholar
  57. 57.
    Hatch, F.T., F.T. Lindgren, L.C. Jensen, G.L. Adamson, A.W. Wong, and R.I. Levy (1973). Quantitative agarose gelelectrophoresis of plasma lipoproteins: A simple technique and two methods for standardization. J. Lab. Clin. Med., 81, 946–960.PubMedGoogle Scholar
  58. 58.
    Davies, B.J. (1964). Method and application to human serum proteins. Ann. N.Y. Acad. Sci., 121, 404–427.CrossRefGoogle Scholar
  59. 59.
    Weber, K., and M. Osborn (1969). The reliability of molecular weight determinations by dodecyl sulphate-polyacrylamide gelelectrophoresis. J. Biol. Chem., 244, 4406–4412.PubMedGoogle Scholar
  60. 60.
    Vesterberg, O. (1972). Isoelectric focusing of proteins in polyacrylamide gels. Biochim. Biophys. Acta, 257, 11–19.PubMedCrossRefGoogle Scholar
  61. 61.
    Awdeh, Z.L., A.R. Williamson, and B.A. Askonas (1968). Isoelectric focusing in polyacrylamide gel and its application to immunoglobulins. Nature, 219, 66–67.PubMedCrossRefGoogle Scholar
  62. 62.
    Scanu, A., J. Toth, C. Edelstein, S. Koga and E. Stiller (1969). Fractionation of human serum high density lipoproteins in urea solutions. Evidence for polypeptide heterogeneity Biochemistry, 8, 3309–3316.CrossRefGoogle Scholar
  63. 63.
    Shore, V., and B. Shore (1967). Some physical and chemical studies on the protein moiety of a high density (1.126–1.195 g/ml) lipoprotein fraction of human serum. Biochemistry, 6, 1962–1969.PubMedCrossRefGoogle Scholar
  64. 64.
    Gros, C. and B. Labouesse (1969). Study of the dansylation reaction of amino acids, peptides and proteins. Eur. J. Biochem., 7, 463–470.PubMedCrossRefGoogle Scholar
  65. 65.
    Zanetta, J.P., G. Vincendon, P. Mandel, and G. Gombos (1970). The utilisation of 1-dimethylaminonaphthalene-5-sulphonyl chloride for quantitative determination of free amino acids and partial analysis of primary structure of proteins. J. Chromatog., 51, 441.CrossRefGoogle Scholar
  66. 66.
    Ambler, G. (1964). Enzymic hydrolysis with carboxy peptidases. Methods Enzymol., 11, 155–166.CrossRefGoogle Scholar
  67. 67.
    Spackman, D.H. (1967) Accelerated amino acid analysis. Methods Enzymol., 11, 3–15.CrossRefGoogle Scholar
  68. 68.
    Hirs, C.H.W. (1967). Determination of cystine as cysteic acid. Methods Enzymol., 11, 59–62.CrossRefGoogle Scholar
  69. 69.
    Liu, T.Y., Y.H. Chang (1971). Hydrolysis of protein with p-toluenesulfonic acid. Determination of tryptophan. J. Biol. Chem., 246, 2842–2848.PubMedGoogle Scholar
  70. 70.
    Rosseneu, M.Y., F. Soetewey, V. Blaton, J. Lievens, and H. Peeters (1974). Microcalorimetric study of phospholipid binding to human apoHDL. Chem. Phys. Lipids, 13, 203–214.CrossRefGoogle Scholar
  71. 71.
    Delahunty, T., H. Nordean Baker, A.M. Gotto, and R.L. Jackson (1975). The primary structure of human plasma high density apolipoprotein Glutamine I (apo-A-I). J. Biol. Chem., 2718-2724.Google Scholar
  72. 72.
    Ewing, A.M., N.K. Freeman, and F.T. Lindgren (1965). The analysis of human serum lipoprotein distributions. In: Advances in Lipid Research, ed. R. Paoletti and D. Kritchevsky, 3, 25–61, Acad. Press, New York.Google Scholar
  73. 73.
    Walton, K., V. Blaton, and H. Peeters (1975). Immunological properties of the plasma lipoproteins in the non human primates. Biochim. Biophys. Acta, in press.Google Scholar
  74. 74.
    Scanu, A.M., C. Edelstein, L. Vitello, R. Jones, and R. Wissler (1973). The serum high density lipoproteins of macacus rhesus. I. Isolation, composition and properties. J. Biol. Chem., 248, 7648–7652.PubMedGoogle Scholar
  75. 75.
    Peeters, H., V. Blaton, and F.T. Lindgren (1973). Dietary induced hyperlipoproteinaemia in chimpanzees. Circulation, 48, Suppl. 4, p. IV–203.Google Scholar
  76. 76.
    Scanu, A. (1975). Polypeptide A-II of serum high density lipoproteins: A lipophilic protein of phylogenetic interest. PAABS Revista, 4, 1–11.Google Scholar

Copyright information

© Springer Science+Business Media New York 1976

Authors and Affiliations

  • V. Blaton
    • 1
  • H. Peeters
    • 1
  1. 1.Simon Stevin Instituut voor Wetenschappelijk OnderzoekBruggeBelgium

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