Abstract
At the onset of this discussion it is important to define what I mean by endoplasmic reticulum and outer mitochondrial membrane. As most of biochemists I use the term endoplasmic reticulum as re-ferred to the microsomal fraction obtained by conventional differential centrifugation at 105,000 x g for one hour, after sedimentation of mitochondria. This fraction is heterogeneous and can be subfractionated by different means (1,2). The distribution of certain enzymes, however, can be regarded as sufficiently homogeneous throughout the different endoplasmic reticulum vescicles to be considered safe the use of such enzymes as markers of microsomes. These enzymes include glucose-6-phosphatase, NADPH cytochrome c reductase and a number of NADPH-linked functions of microsomes as well as cytochrome P450 (3). By outer mitochondrial membrane, on the other hand, I mean a fraction derived from isolated mitochondria, subjected to treatments capable to detach the outer from the inner membrane and subsequently fractionated either by continuous or discontinuous density-gradient as well as by differential centrifugation (3–12).
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Sottocasa, G.L. (1971). Similarities and Dissimilarities between Outer Mitochondrial Membrane and Endoplasmic Reticulum. In: Porcellati, G., di Jeso, F. (eds) Membrane-Bound Enzymes. Advances in Experimental Medicine and Biology, vol 14. Springer, Boston, MA. https://doi.org/10.1007/978-1-4614-4616-3_14
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